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Brain proteomic differences between wild-type and CD44- mice induced by chronic Toxoplasma gondii infection

Original Paper
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Abstract

Chronic clinical Toxoplasma gondii (T. gondii) infection is the primary disease state that causes severe encephalitis. CD44 is a member of the cell adhesion molecule family and plays an important role in T. gondii infection. However, proteomic changes in CD44 during chronic T. gondii infection have rarely been reported. Thus, an iTRAQ-based proteomic study coupled with 2D-LC-MS/MS analysis was performed to screen CD44-related proteins during chronic T. gondii infection. As a result, a total of 2612 proteins were reliably identified and quantified. Subsequently, 259, 106, and 249 differentially expressed proteins (DEPs) were compared between CD44- mice (A) vs wild-type mice (B), B vs wild-type mice infected with T. gondii (C), and C vs CD44- mice infected with T. gondii (D). Gene ontology, KEGG pathway, and protein-protein interaction analyses were performed on the DEPs. According to the results, immune-related proteins were altered significantly among the A vs B, B vs C, and C vs D comparisons, which might indicate that chronic T.  gondii infection caused changes in the host immune response. Additionally, Ca2+- and metabolism-related proteins were upregulated in C vs D, which supported the hypothesis that CD44 mediated the production of host Ca2+ and IFN-γ and that the parasite preferentially invaded cells expressing high levels of CD44. The present findings validate and enable a more comprehensive knowledge of the role of CD44 in hosts chronically infected with T. gondii, thus providing new ideas for future studies on the specific functions of CD44 in latent toxoplasmosis.

Keywords

Toxoplasma gondii CD44- iTRAQ Proteomic Differentially expressed protein (DEP) 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (R.F., No. 31572510), the Natural Science Foundation of Hubei Province (Grant No. 2017CFA020), and Da Bei Nong Group Promoted Project for Young Scholar of HZAU (Grant No. 2017DBN001).

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Supplementary material

436_2018_5954_MOESM1_ESM.pdf (33 kb)
Table S1 Mouse proteins identified at critical false discovery rates (Critical FDR < 0.01). (PDF 33 kb)
436_2018_5954_MOESM2_ESM.xls (2.5 mb)
Table S2 List of 2612 trusted proteins identified and quantified in this study. (XLS 2572 kb)
436_2018_5954_MOESM3_ESM.xls (137 kb)
Table S3 List of differentially expressed proteins (DEPs) between CD44- and wild-type mice (fold change > 2 or fold change < 0.5). (XLS 137 kb)
436_2018_5954_MOESM4_ESM.xls (66 kb)
Table S4 List of differentially expressed proteins (DEPs) between wild-type mice and wild-type mice infected with T. gondii (fold change > 2 or fold change < 0.5). (XLS 66 kb)
436_2018_5954_MOESM5_ESM.xls (130 kb)
Table S5 List of differentially expressed proteins (DEPs) between wild-type mice infected with T. gondii and CD44- mice infected with T. gondii(fold change > 2 or fold change < 0.5). (XLS 129 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Agricultural Microbiology, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Hubei Centre for Animal Diseases Control and PreventionWuhanPeople’s Republic of China
  3. 3.Hubei Provincial Centre for Diseases Control and PreventionWuhanPeople’s Republic of China

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