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Three new species of Clinostomum Leidy, 1856 (Trematoda) from Middle American fish-eating birds

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Abstract

We recently engaged in a two-part study of Clinostomum Leidy, 1856 across a geographic range comprising central Mexico southwards to Costa Rica, in Central America. In the first study, we investigated the species boundaries by using DNA sequences of mitochondrial and nuclear molecular markers, implementing several analytical tools and species delimitation methods. The result of that approach revealed five highly divergent genetic lineages that were interpreted as independent evolutionary units, or species. Here, we present the second part of the study, where we describe three of the five species for which we have sexually mature adult specimens obtained from the mouth cavity of fish-eating birds. Additionally, we characterise morphologically the metacercariae of the other two species, collected from freshwater fishes; these species cannot be formally described since no adults were found in their definitive hosts. We further discuss the characters that are more reliable for species identification within Clinostomum, such as the cirrus sac shape and relative position with respect to testes and ovary, the shape of the reproductive organs, and the diverticulated condition of the caeca.

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Acknowledgments

We thank Robert Poulin for the review of an earlier version of the manuscript. We also thank Leopoldo Andrade, Eduardo Hernández, and Jesus Hernández for their help during field work in Mexico and Costa Rica; Luis García for providing specimens for comparison deposited at the Colección Nacional de Helmintos; and Berenit Mendoza for her help processing the samples for the SEM study. Hosts in Mexico were collected under the Cartilla Nacional de Colector Científico (FAUT 0202 and 0057) issued by the Secretaría del Medio Ambiente y Recursos Naturales (SEMARNAT), to MGV and GPPL, respectively. We thank Arturo Angulo for his help to collect fish specimens in Costa Rica under the collecting permit issued by the Costa Rican government.

Funding

This research was supported by grants from the Programa de Apoyo a Proyectos de Investigación e Inovación Tecnológica (PAPIIT-UNAM) I202617 and IN206716 to MGV and GPPL, respectively.

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Authors and Affiliations

  1. Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Ap. Postal 70-153, C.P., 04510, México, Mexico

    Ana L. Sereno-Uribe, Martín García-Varela & Gerardo Pérez-Ponce de León

  2. Investigador Cátedra CONACyT, Instituto de Ecología, A.C., Red de Estudios Moleculares Avanzados, Km 2.5 Ant. Carretera a Coatepec, Xalapa, 91070, Veracruz, Mexico

    Carlos D. Pinacho-Pinacho

Authors
  1. Ana L. Sereno-Uribe
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  2. Martín García-Varela
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  3. Carlos D. Pinacho-Pinacho
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  4. Gerardo Pérez-Ponce de León
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Corresponding author

Correspondence to Gerardo Pérez-Ponce de León.

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Sereno-Uribe, A.L., García-Varela, M., Pinacho-Pinacho, C.D. et al. Three new species of Clinostomum Leidy, 1856 (Trematoda) from Middle American fish-eating birds. Parasitol Res 117, 2171–2185 (2018). https://doi.org/10.1007/s00436-018-5905-8

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  • DOI: https://doi.org/10.1007/s00436-018-5905-8

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