Abstract
Lungworms of the genus Dictyocaulus Railliet and Henry, 1907 (Nematoda: Trichostrongyloidea) are the causative agents of parasitic bronchitis (dictyocaulosis, husk) of various ungulate hosts, including domestic and wild ruminants. Correct diagnosis of lungworm species and a better understanding of the transmission patterns of Dictyocaulus spp. are crucial in minimising the risk of its cross transmission between wildlife and livestock, and for the control of dictyocaulosis. The study was conducted on large lungworms collected from European bison, roe deer and red deer. The study resulted in 14 sequences of the partial cox1 region of Dictyocaulus spp. and 10 novel DNA sequences of partial cox3 region, including the first available mt cox3 sequence, of the roe deer lungworm (D. capreolus). The European bison was infected with bison genotype of D. viviparus, whereas red deer and roe deer were infected with D. cervi and D. capreolus respectively. The current study revealed that the cox3 nucleotide sequences of D. capreolus and D. viviparus were 100% homologous to each other. Our findings indicate that the mt cox3 gene does not serve as an efficient mt marker for systematic, population genetic or molecular epidemiological studies of Dictyocaulus lungworms.
References
Ács Z, Hayward A, Sugár L (2016) Genetic diversity and population genetics of large lungworms (Dictyocaulus, Nematoda) in wild deer in Hungary. Parasitol Res 115:3295–3312
Blouin MS (1998) Mitochondrial DNA diversity in nematodes. J Helminthol 72:285–289
Carreno RA, Diez-Baños N, del Rosario Hidalgo-Argüello M, Nadler SA (2009) Characterisation of Dictyocaulus species (Nematoda: Trichostrongyloidea) from three species of wild ruminants in northwestern Spain. J Parasitol 95:966–970
Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest2: more models, new heuristics and parallel computing. Nat Methods 9:772
David GP (1997) Survey on lungworms in adult cattle. Vet Rec 141:343–344
Divina BP, Höglund J (2002) Heterologous transmission with Dictyocaulus capreolus from roe deer (Capreolus capreolus) to cattle (Bos taurus). J Helminthol 76:125–138
Epe C, Samson-Himmelstherna GV, Schnider T (1997) Differences in a ribosomal DNA sequence of lungworm species (Nematoda: Dictyocaulidae) from fallow deer, cattle, sheep and donkeys. Res Vet Sci 62:17–21
Eysker M, van Miltenburg L (1988) Epidemiological patterns of gastrointestinal and lung helminth infections in grazing calves in the Netherlands. Vet Parasitol 29:29–39
Eysker M (1994) Dictyocaulosis in cattle. Comp Cont Educ Proct 16:669–675
Gasser RB, Jabbar A, Mohandas N, Höglund J, Hall RS, Littlewood DT, Jex AR (2012) Assessment of the genetic relationship between Dictyocaulus species from Bos taurus and Cervus elaphus using complete mitochondrial genomic datasets. Parasit Vectors 5:241
Guindon S, Gascuel O (2003) A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Syst Biol 52:696–704
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I for diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–299
Gibbons LM, Höglund J (2002) Dictyocaulus capreolus n. sp. (Nematoda: Trichostrongyloidae) from roe deer, Capreolus capreolus and moose, Alces alces in Sweden. J Helminthol 76:119–124
Hu M, Höglund J, Chilton NB, Zhu X, Gasser R (2002) Mutation scanning analysis of mitochondrial cytochrome c oxidase subunit 1 reveals limited gene flow among bovine lungworm subpopulations in Sweden. Electrophoreses 23:3357–3363
Höglund J, Wilhelmsson E, Christensson D, Mörner T, Waller P, Mattsson JG (1999) ITS2 sequences of Dictyocaulus species from cattle, roe deer and moose in Sweden: molecular evidence for a new species. Int J Parasitol 29:607–611
Höglund J, Morrison DA, Divina BP, Wilhelmsson E, Mattson JG (2003) Phylogeny of Dictyocaulus (lungworms) from eight species of ruminants based on analyses of ribosomal RNA data. Parasitology 127:179–187
Höglund J, Morrison DA, Mattsson JG, Engström A (2006) Population genetics of the bovine/cattle lungworm (Dictyocaulus viviparous) based on mtDNA and AFLP marker techniques. Parasitology 133:89–99
Mahmood F, Khan A, Hussain R, Anjum MS (2014) Prevalence and pathology of Dictyocaulus viviparus infection in cattle and buffaloes. J Anim Plant Sci 24:743–748
Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755
Panuska C (2006) Lungworms of ruminants. Vet Clin N Am-Food A 22:583–593
Pyziel AM (2014) Molecular analysis of lungworms from European bison (Bison bonasus) on the basis of small subunit ribosomal RNA gene (SSU). Acta Parasitol 59:122–125
Pyziel AM, Laskowski Z, Höglund J (2015) Development of a multiplex PCR for identification of Dictyocaulus lungworms in domestic and wild ruminants. Parasitol Res 114:3923–3926
Pyziel AM, Laskowski Z, Demiaszkiewicz AW, Höglund J (2017) Interrelationships of Dictyocaulus spp. in wild ruminants with morphological description of Dictyocaulus cervi n. sp. (Nematoda: Trichostrongyloidea) from red deer, Cervus elaphus. J Parasitol 103:506–518
Rahko T, Saari S, Nikander S (1992) Histopathological lesions in spontaneous dictyocaulotic pneumonia of reindeer (Rangifer tarandus tarandus L.). Rangifer 12:115–122
Romano C, Persiani A (1982) Reporting a new host, Dama dama, for Dictyocaulus (Micrurocaulus) eckerti Skrjabin, 1931. Parassitologia 24:205–209
Skrjabin KI, Shikhobalova NP, Schultz RS (1954) Dictyocaulidae, Heligosomatidae and Ollulanidae in animals. In: Essentials of nematology. Academy of Sciences, Moscow, Russia, pp 36–41
Funding
The presented findings were funded by the National Science Centre of Poland, in the framework of Miniatura, grant no. 2017/01/X/NZ6/00262.
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Pyziel, A.M., Laskowski, Z. & Höglund, J. An assessment of the use of cox1 and cox3 mitochondrial genetic markers for the identification of Dictyocaulus spp. (Nematoda: Trichostrongyloidea) in wild ruminants. Parasitol Res 117, 2341–2345 (2018). https://doi.org/10.1007/s00436-018-5904-9
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DOI: https://doi.org/10.1007/s00436-018-5904-9