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Parasitology Research

, Volume 117, Issue 5, pp 1521–1534 | Cite as

Comparison of recombinant cathepsins L1, L2, and L5 as ELISA targets for serodiagnosis of bovine and ovine fascioliasis

  • Victoria Martínez-Sernández
  • María J. Perteguer
  • Ana Hernández-González
  • Mercedes Mezo
  • Marta González-Warleta
  • Ricardo A. Orbegozo-Medina
  • Fernanda Romarís
  • Esperanza Paniagua
  • Teresa Gárate
  • Florencio M. Ubeira
Original Paper

Abstract

Infections caused by Fasciola hepatica are of great importance in the veterinary field, as they cause important economic losses to livestock producers. Serodiagnostic methods, typically ELISA (with either native or recombinant antigens), are often used for early diagnosis. The use of native antigens, as in the MM3-SERO ELISA (commercialized as BIO K 211, BIO-X Diagnostics), continues to be beneficial in terms of sensitivity and specificity; however, there is interest in developing ELISA tests based on recombinant antigens to avoid the need to culture parasites. Of the antigens secreted by adult flukes, recombinant procathepsin L1 (rFhpCL1) is the most commonly tested in ELISA to date. However, although adult flukes produce three different clades of CLs (FhCL1, FhCL2, and FhCL5), to our knowledge, the diagnostic value of recombinant FhCL2 and FhCL5 has not yet been investigated. In the present study, we developed and tested three indirect ELISAs using rFhpCL1, rFhpCL2, and rFhpCL5 and evaluated their recognition by sera from sheep and cattle naturally infected with F. hepatica. Although the overall antibody response to these three rFhpCLs was similar, some animals displayed preferential recognition for particular rFhpCLs. Moreover, for cattle sera, the highest sensitivity was obtained using rFhpCL2 (97%), being equal for both rFhpCL1 and rFhpCL5 (87.9%), after adjusting cut-offs for maximum specificity. By contrast, for sheep sera, the sensitivity was 100% for the three rFhpCLs. Finally, the presence of truncated and/or partially unfolded molecules in antigen preparations is postulated as a possible source of cross-reactivity.

Keywords

Cathepsin Fasciola hepatica Fascioliasis ELISA Cross-reactivity Serodiagnosis 

Notes

Funding

This work was supported by the Ministerio de Economía y Competitividad (Spain) [grant number AGL2011-30563-C03], Ministerio de Economía, Industria y Competitividad (INIA, Spain) [grants numbers RTA2017-00010-C02-01 and RTA2017-00010-C02-02], ISCIII-AESI (Spain) [project MPY 1279/15], Instituto de Salud Carlos III (Spain) [agreement PI14CIII/00076], and the Consellería de Cultura, Educación e Ordenación Universitaria (Xunta de Galicia, Spain) [grant number ED431B 2017/18]. VMS holds a predoctoral fellowship from the Spanish Ministerio de Educación, Cultura y Deporte (Programa de Formación del Profesorado Universitario). RAOM holds a predoctoral fellowship from the Spanish Ministerio de Economía y Competitividad (Programa de Formación de Personal Investigador). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Compliance with ethical standards

Blood and fecal samples were collected from naturally infected sheep and cattle by veterinarians from the “Centro de Investigaciones Agrarias de Mabegondo” (INGACAL, A Coruña, Spain). The samples were collected either during routine control and treatment of herds (sheep) or in the slaughterhouse (cattle). All procedures were carried out in strict accordance with Spanish and EU legislation (Law 32/2007, R.D. 53/2013, and Council Directive 2010/63/EU).

Conflict of interests

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Victoria Martínez-Sernández
    • 1
  • María J. Perteguer
    • 2
  • Ana Hernández-González
    • 2
  • Mercedes Mezo
    • 3
  • Marta González-Warleta
    • 3
  • Ricardo A. Orbegozo-Medina
    • 1
  • Fernanda Romarís
    • 1
  • Esperanza Paniagua
    • 1
  • Teresa Gárate
    • 2
  • Florencio M. Ubeira
    • 1
  1. 1.Laboratorio de Parasitología, Facultad de FarmaciaUniversidad de Santiago de CompostelaSantiago de CompostelaSpain
  2. 2.Centro Nacional de MicrobiologíaInstituto de Salud Carlos IIIMajadahondaSpain
  3. 3.Laboratorio de Parasitología, Centro de Investigaciones Agrarias de MabegondoINGACALAbegondoSpain

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