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Parasitology Research

, Volume 117, Issue 4, pp 1257–1263 | Cite as

Human cystic echinococcosis in Turkey: a preliminary study on DNA polymorphisms of hydatid cysts removed from confirmed patients

  • Serra Orsten
  • Belgees Boufana
  • Turkmen Ciftci
  • Devrim Akinci
  • Ergun Karaagaoglu
  • Cumhur Ozkuyumcu
  • Adriano Casulli
  • Okan Akhan
Original Paper

Abstract

Cystic echinococcosis caused by the larval stages of Echinococcus granulosus sensu lato s.l is endemic in Turkey with a high public health impact particularly in rural areas. The aim of this study was to investigate the genetic variation and population structure of E. granulosus s.s using metacestode isolates removed from surgically confirmed patients originating from several regions in Turkey and to investigate the occurrence of autochthonous transmission. Using DNA extracted from a total of 46 human-derived CE isolates, we successfully analysed an 827-bp fragment within the cox1 mitochondrial gene and confirmed the causative agent of human cystic echinococcosis in patients included in this study to be Echinococcus granulosus s.s (G1 and G3 genotypes). The haplotype parsimony network consisted of 28 haplotypes arranged within three main clusters and the neutrality indices were both negative and significant indicating negative selection or population expansion. The assessment carried out in this study using GenBank nucleotide sequence data from Turkey for sheep and cattle hosts demonstrated the importance of autochthonous transmission with sheep, cattle and humans harbouring the same haplotypes. Further studies are required to investigate the biological significance, if any, of E. granulosus s.s haplotypes and the genetic variability of CE from human patients using longer nucleotide sequences and a larger sample set.

Keywords

Echinococcus granulosus sensu stricto Human cystic echinococcosis Population structure Genetic variation Autochthonous transmission Turkey 

Notes

Funding information

This study was supported by the European Community’s Seventh Framework Programme under the grant agreement 602051 (HERACLES Project; http://www.Heracles-fp7.eu/) and in part by the Overseas Research Fellowship Program (2214) of the Scientific and Technological Research Council of Turkey.

Compliance with ethical standards

The current study was approved by the Ethical Committee of the Faculty of Medicine, Hacettepe University, Ankara, Turkey (GO 14/293-37).

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Health ServicesHacettepe UniversityAnkaraTurkey
  2. 2.Department of Infectious Diseases, WHO Collaborating Centre for the Epidemiology, Detection and Control of CE and AE, European Union Reference Laboratory for ParasitesIstituto Superiore di SanitáRomeItaly
  3. 3.Faculty of Medicine, Department of RadiologyHacettepe UniversityAnkaraTurkey
  4. 4.Faculty of Medicine, Department of BiostatisticsHacettepe UniversityAnkaraTurkey
  5. 5.Faculty of Medicine, Department of Medical MicrobiologyHacettepe UniversityAnkaraTurkey

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