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Parasitology Research

, Volume 117, Issue 4, pp 1245–1256 | Cite as

Further aspects of Toxoplasma gondii elimination in the presence of metals

  • Laís Pessanha de Carvalho
  • Edésio José Tenório de Melo
Original Paper
  • 140 Downloads

Abstract

Toxoplasma gondii, the etiological agent of toxoplasmosis, infects nucleated cells and then resides and multiplies within a parasitophorous vacuole. For this purpose, the parasite secretes many virulence factors for the purpose of invading and subverting the host microbicidal defenses in order to facilitate its survival in the intracellular milieu. Essential metals are structural components of proteins and enzymes or cofactors of enzymatic reactions responsible for these parasitic survival mechanisms. However, an excess of non-essential or essential metals can lead to parasite death. Thus, infected host cells were incubated with 20 μM ZnCl2 in conjunction with 3 μM CdCl2 or HgCl2 for 12 h in order to investigate cellular events and organelle damage related to intracellular parasite death and elimination. In the presence of these metals, the tachyzoites undergo lipid uptake and transport impairment, functional and structural mitochondrial disorders, DNA condensation, and acidification of the parasitophorous vacuole, thus leading to parasite death. Additional research has suggested that lysosome-vacuole fusion was involved in parasite elimination since acid phosphatases were found inside the parasitophorous vacuole, and vacuoles containing parasites were also positive for autophagy. In conclusion, low concentrations of CdCl2, HgCl2, and ZnCl2 can cause damage to Toxoplasma gondii organelles, leading to loss of viability, organelle death, and elimination without causing toxic effects to host cells.

Keywords

Cadmium Essential metal Mercury Nonessential metals Toxoplasma gondii Zinc 

Notes

Acknowledgements

The authors would like to thank FAPERJ (Fundação de Amparo à Pesquisa do Rio de Janeiro) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Laís Pessanha de Carvalho
    • 1
  • Edésio José Tenório de Melo
    • 1
  1. 1.Laboratory of Tissue and Cell BiologyState University of North Fluminense—Darcy RibeiroRio de JaneiroBrazil

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