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Parasitology Research

, Volume 117, Issue 4, pp 1147–1158 | Cite as

Mast cell-nerve interaction in the colon of Trypanosoma cruzi-infected individuals with chagasic megacolon

  • Patrícia Rocha Martins
  • Rodolfo Duarte Nascimento
  • Aline Tomaz dos Santos
  • Enio Chaves de Oliveira
  • Patricia Massara Martinelli
  • Débora d’Avila Reis
Original Paper

Abstract

Chagas disease is an infection caused by the parasite Trypanosoma cruzi that affects millions of people worldwide and is endemic in Latin America. Megacolon is the most frequent complication of the digestive chronic form and happens due to lesions of the enteric nervous system. The neuronal lesions seem to initiate in the acute phase and persist during the chronic phase, albeit the mechanisms involved in this process are still debated. Among the cells of the immune system possibly involved in this pathological process is the mast cell (MC) due to its well-known role in the bi-directional communication between the immune and nervous systems. Using ultrastructural analysis, we found an increased number of degranulated MCs in close proximity to nerve fibers in infected patients when compared with uninfected controls. We also immunostained MCs for the two pro-inflammatory molecules tryptase and chymase, the first being also important in neuronal death. The number of MCs immunostained for tryptase or chymase was increased in patients with megacolon, whereas increased tryptase staining was additionally observed in patients without megacolon. Moreover, we detected the expression of the tryptase receptor PAR2 in neurons of the enteric nervous system, which correlated to the tryptase staining results. Altogether, the data presented herein point to the participation of MCs on the denervation process that occurs in the development of T. cruzi-induced megacolon.

Keywords

Neuroimmune interaction Tryptase and chymase mast cells PAR2 Enteric nervous system Chagasic megacolon 

Notes

Funding information

This work was supported by the Conselho Nacional de desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Patrícia Rocha Martins
    • 1
    • 2
  • Rodolfo Duarte Nascimento
    • 3
  • Aline Tomaz dos Santos
    • 1
  • Enio Chaves de Oliveira
    • 4
  • Patricia Massara Martinelli
    • 1
  • Débora d’Avila Reis
    • 1
  1. 1.Morphology DepartmentFederal University of Minas GeraisBelo HorizonteBrazil
  2. 2.Centre for Education and Research at Institute Mario PennaBelo HorizonteBrazil
  3. 3.Campus Governador ValadaresFederal University of Juiz de ForaJuiz de ForaBrazil
  4. 4.Federal University of GoiasGoiâniaBrazil

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