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A new microsporidium Fibrillaspora daphniae g. n. sp. n. infecting Daphnia magna (Crustacea: Cladocera) in Siberia and its taxonomic placing within a new family Fibrillasporidae and new superfamily Tubulinosematoidea (Opisthosporidia: Microsporidia)

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A Correction to this article was published on 23 February 2018

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Abstract

Infection with a new microsporidium, Fibrillaspora daphniae g. n. sp. n., was found in a local Daphnia magna population in Tomsk region (Western Siberia, Russia) at the prevalence rate of 52%. Histological sections showed parasite cells entirely encompassing the host haemocoel. Methanol-fixed spores were elongate, oval, 4.8 ± 0.3 μm × 2.3 ± 0.2 μm in size. All developmental stages were in direct contact with the host cell cytoplasm, with single nuclei, and division by binary fission. The sporont surface was covered with an additional outer layer composed of fine tubules. The spores possessed a thick endospore, large posterior vacuole filled with electron-dense granules, and a bipartite polaroplast composed of anterior lamellar and posterior globular elements. The polar tube was slightly anisofilar, with 13–19 coils arranged in one row; the two posterior coils were of lesser diameter. The small subunit ribosomal RNA gene sequence was deposited in Genbank under accession # MF278272. Considering the sister relationship between Fibrillanosema crangonycis and our new isolate described here as Fibrillaspora daphniae, we propose a new family Fibrillasporidae fam. n. to contain these two genera and the descendants of their common ancestor. A new superfamily Tubulinosematoidea superfam. n. is proposed as a monophyletic assemblage of Fibrillasporidae fam. n. and Tubulinosematidae.

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Change history

  • 23 February 2018

    Ultrastructure of Fibrillaspora is highly similar to that of Berwaldia but the former is phylogenetically distant from the taxon annotated as Berwaldia schaefernai (Genbank accession # AY090042). It was shown recently, however, that the latter sequence was not derived from Berwaldia, and correct nucleotide sequence data were provided for Berwaldia. This allowed recondideration of Fibrillaspora as a junior synonym of Berwaldia and redefinition of Fibrillaspora daphniae as a new combination, Berwaldia daphniae comb. nov.

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Acknowledgements

Authors are indebted to A.A. Miller (Institute of Strength Physics and Materials Science of Siberian Branch Russian Academy of Sciences, Tomsk, Russia) for assistance with transmission electron microscopy and to Theodore G. Andreadis (The Connecticut Agricultural Experiment Station, CT, USA) for language correction of the manuscript. The research is performed within the frames of Governmental Contract from Ministry of Education and Science of Russian Federation # 6.7525.2017/8.9 and a grant from Russian Foundation of Basic Research # 17-04-00871.

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Authors and Affiliations

  1. Tomsk State University, pr. Lenina 36, Tomsk, 634050, Russia

    Anastasia V. Simakova

  2. All-Russian Institute for Plant Protection, sh. Podbelskogo 3, St. Petersburg, Pushkin, 196608, Russia

    Anastasia V. Simakova, Yuri S. Tokarev & Irma V. Issi

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  1. Anastasia V. Simakova
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  2. Yuri S. Tokarev
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Correspondence to Yuri S. Tokarev.

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Simakova, A.V., Tokarev, Y.S. & Issi, I.V. A new microsporidium Fibrillaspora daphniae g. n. sp. n. infecting Daphnia magna (Crustacea: Cladocera) in Siberia and its taxonomic placing within a new family Fibrillasporidae and new superfamily Tubulinosematoidea (Opisthosporidia: Microsporidia). Parasitol Res 117, 759–766 (2018). https://doi.org/10.1007/s00436-018-5749-2

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