Parasitology Research

, Volume 116, Issue 12, pp 3303–3313 | Cite as

Morphological and molecular description of Myxobolus batalhensis n. sp. (Myxozoa, Myxosporea), a liver and ovary parasite of Salminus hilarii in Brazil

  • Diego Henrique Mirandola Dias VieiraEmail author
  • Gema Alama-Bermejo
  • Jerri Lee Bartholomew
  • Vanessa Doro Abdallah
  • Rodney Kozlowiski de Azevedo
Original Paper


Plasmodia containing myxospores belonging to the genus Myxobolus Bütschli, 1882 were found in the ovaries and liver of Salminus hilarii. Despite its economic value, this fish host has no previous reports of myxozoan infections. Herein, we describe Myxobolus batalhensis n. sp. using morphological and ultrastructural data, as well as histological and SSU rDNA molecular data. The mature myxospores were elongated, measuring in average 15.2 ± 0.8 μm in length, 8.4 ± 0.4 μm in width, and 5.1 ± 0.2 μm in thickness. Polar capsules were elongated and measured 5.3 ± 0.3 μm in length and 2.8 ± 0.3 μm in width. Polar filaments had 6–9 coils. Histopathological analysis showed coagulation necrosis associated with cell lysis as a response of the host cell to the parasite in the ovaries. No inflammatory reaction was observed in the liver, although the presence of the plasmodia caused changes in tissue structure. The phylogenetic analysis of South American myxobolid species showed M. batalhensis n. sp. as sister species of Myxobolus aureus. This is the first report of a myxozoan species parasitizing S. hilarii and the first myxozoan species described in the Batalha river.


Bryconidae Histopathology Myxobolidae Phylogeny SSU rDNA Ultrastructure 



We are grateful to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Process: 2013/25623-0, 2014/12862-0, 2015/01361-2, and 2015/24901-2), Czech Science Foundation (14–28784P) and Consellería de Educación, Cultura y Deporte, Valencia, Spain (APOSTD/2013/087). We would like to thank Reinaldo José da Silva, Marianna Vaz Rodrigues, and Maria Isabel Müller for their support during this research.

Compliance with ethical standards

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Diego Henrique Mirandola Dias Vieira
    • 1
    Email author
  • Gema Alama-Bermejo
    • 2
    • 3
  • Jerri Lee Bartholomew
    • 2
  • Vanessa Doro Abdallah
    • 4
  • Rodney Kozlowiski de Azevedo
    • 4
  1. 1.Department of ParasitologyUniversidade Estadual Paulista “Júlio de Mesquita Filho” – UNESPSão PauloBrazil
  2. 2.Department of MicrobiologyOregon State UniversityCorvallisUSA
  3. 3.Center for Applied Research and Technology Transference in Marine Resources Almirante Storni (CIMAS-CCT CONICET) & ESCiMarNational University of ComahueSan Antonio OesteArgentina
  4. 4.Department of Environmental SciencesUniversidade do Sagrado Coração – USCSão PauloBrazil

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