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Myxobolus sp. and Henneguya sp. (Cnidaria: Myxobolidae) natural co-infection in the kidney of Piaractus mesopotamicus (Characiformes: Serrasalmidae)

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Abstract

This study evaluated the myxozoan infection and histopathology of the kidney of freshwater fish Piaractus mesopotamicus from intensive fish farming in Brazil. A total of 55 fish were examined for myxozoan infection. Infected organs were processed by usual histology and stained with hematoxylin-eosin (H&E) and Ziehl-Neelsen (ZN). From the total of 55 fish analyzed, 47 (85.45%) presented myxospores, being 9.09% (5/55) only with Myxobolus sp., 5.45% (3/55) only with Henneguya sp., and 70.91% (39/55) presenting both parasites. The presence of myxospores was associated with histological alterations in both stromal and renal parenchyma. Myxospores were found mostly in the peritubular interstitial tissue and in low intensity in the glomerulus which caused nuclear hypertrophy and loss of Bowman space. An increase in the glomerular tuft and a reduction in the lumen of the collector tubules were also observed, besides the high number of melanomacrophage cells in the glomerulus. This study reports for the first time detection of myxozoan mixed infection in one organ of pacu and discuss the possible transportation of myxospores in the circulating blood.

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Acknowledgements

The authors thank the National Council of Scientific and Technological Development (CNPq) for the grant to M.L. Martins (CNPq 305869-2014-8) and the Hungarian partner supported by the grant OTKA K1000132.

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Authors and Affiliations

  1. Department of Veterinary Pathology, Brazil Universit, Descalvado Campus, Av. Hilário da Silva Passos, 950, Parque Universitário Descalvado, São Paulo, 13690-970, Brazil

    Wilson Gómez Manrique & Marco Antonio de Andrade Belo

  2. Department of Clinical Analysis, Toxicology and Food Science, Laboratory of Virology, University of São Paulo, Bloco S, 1° Andar, Av. do Café, s/n, Ribeirão Preto, 14040-903, Brazil

    Mayra Araguaia Pereira Figueiredo

  3. AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, Florianópolis, Florianópolis, SC, 88040-900, Brazil

    Maurício Laterça Martins

  4. Hungarian Academy of Sciences, Veterinary Medical Research Institute, PO Box 18, Budapest, 1581, Hungary

    Kálmán Molnár

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  1. Wilson Gómez Manrique
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  2. Mayra Araguaia Pereira Figueiredo
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Correspondence to Wilson Gómez Manrique.

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The euthanasia method was approved by the ethics committee of the Sao Paulo State University—Unesp (CEUA no. 020092/09).

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Manrique, W.G., Figueiredo, M.A.P., de Andrade Belo, M.A. et al. Myxobolus sp. and Henneguya sp. (Cnidaria: Myxobolidae) natural co-infection in the kidney of Piaractus mesopotamicus (Characiformes: Serrasalmidae). Parasitol Res 116, 2853–2860 (2017). https://doi.org/10.1007/s00436-017-5571-2

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