Anticoccidial effects of Artemisia annua ethanolic extract: prevention, simultaneous challenge-medication, and treatment

Abstract

The effect of Artemisia annua ethanolic extract (AE) as a potential source of herbal anticoccidial activity was investigated on experimental coccidiosis in chicken. One hundred ninety-two one-day-old chicks were divided in to 8 groups (n = 24) including AE prevention group, AE-treated group, simultaneously challenged AE-medicated group, challenged-untreated group (positive control), unchallenged-untreated group (negative control), salinomycine prevention group, salinomycine-treated group, and simultaneously challenged salinomycine-medicated group, in a completely randomized design. Oral challenge carried out by a suspension containing a mixture of 200,000 oocysts Eimeria acervulina, 30,000 oocysts Eimeria necatrix, and 20,000 oocysts Eimeria tenella on day 21 of age. Weight gain in AE prevention group significantly increased compared to positive control group (p < 0.05). Unlike salinomycine prevention group, the food conversion ratio (FCR) of AE prevention group was not significantly higher than negative control. Oocyst per gram (OPG) in simultaneously challenged AE-medicated group had no significant difference, while for 38% of the days, in simultaneously challenged salinomycine-medicated group significantly decreased (p < 0.05). The food intake of AE-treated group had no significant difference with salinomycine-treated group (p > 0.05). In half of the days of OPGs sampling, AE-treated group was reduced significantly compared to positive control group (p < 0.05). Collectively, the in vivo study of anticoccidial effects of AE in the prevention section was more effective than the treatment section, while the treatment section was more effective than the simultaneous section. We concluded that AE has a potential value to use as an herbal medicine for preventive measure in chicken coccidiosis.

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References

  1. Abbas TE (2012) Phytogenic feed additives as a Coccidiostat in poultry. Bull Environ, Pharmacol Life Sci 7:22–24

    Google Scholar 

  2. Allen PC, Lydon J, Danforth HD (1997) Effects of components of Artemisia annua on coccidia infections in chickens. Poult Sci 76:1156–1163

    CAS  Article  PubMed  Google Scholar 

  3. Almeida GF, Horsteda K, Thamsborgb SM, Kyvsgaardc NC, Ferreirad JFC, Hermansena JE (2011) Use of Artemisia annua as a natural coccidiostate in free-range broilers and its effects on infection dynamics and performance. Vet Parasitol 186:178–187

    Article  PubMed  Google Scholar 

  4. Arab HA, Rahbari S, Rassouli A, Moslemi MH, Khosravirad F (2006) Determination of artemisinin in Artemisia sieberi and anticoccidial effects of the plant extract in broiler chickens. Trop Anim Health Prod 38:497–503

    CAS  Article  PubMed  Google Scholar 

  5. Arab HA, Mardjanmehr SH, Shahbazfar A, Rassouli A, Abdollahi M, Nekouie O (2009) Toxicopathologic effects of artemisinin in broiler chickens following a single oral dose: an LD50 study. Int J Poult Sci 8:808–812

    CAS  Article  Google Scholar 

  6. Bensegueni AM, Abdeldjelil MC, Agabou A, Redouane-Salah S (2014) Effects of white wormwood (Artemisia herba-alba Asso), during an experimental coccidiosis in broilers. Ann Biol Res 5(3):61–66

    Google Scholar 

  7. Bhakuni RS, Jain DC, Sharma RP, Kumar S (2001) Secondary metabolites of Artemisia annua and their biological activity. Curr Sci 80:35–48

    CAS  Google Scholar 

  8. Brisibe EA, Umoren UE, Owai PU, Brisibe F (2008) Dietary inclusion of dried Artemisia annua leaves for management of coccidiosis and growth enhancement in chickens. Afr J Biotechnol 22:4083–4092

    Google Scholar 

  9. Conway DP, McKenzie ME (2007) Poultry coccidiosis: diagnostic and testing procedures, 3rd edn. Blackwell, USA

    Google Scholar 

  10. Dragan L, Titilincu A, Dan I, Dunca I, Drăgan M, Mircean V (2010) Effects of Artemisia annua and Pimpinella anisum on Eimeria tenella (Phylum Apicomplexa) low infection in chickens. Sci Parasitol 11:77–82

    Google Scholar 

  11. Drăgan L, Györke A, Ferreira JFS, Pop IA, Dunca I, Drăgan M, Mircean V, Dan I, Cozma V (2014) Effects of Artemisia annua and Foeniculum vulgare on chickens highly infected with Eimeria tenella (Phylum Apicomplexa). Acta Vet Scand 56:1–7

    Article  Google Scholar 

  12. Engberg RM, Grevsen K, Ivarsen E, Fretté X, Christensen LR, Højberg O, Jensen BB, Canibe N (2012) The effect of Artemisia annua on broiler performance, on intestinal microbiota and on the course of a Clostridium perfringens infection applying a necrotic enteritis disease model. Avian Pathol 4:369–376

    Article  Google Scholar 

  13. Fatemi AR, Razavi SM, Asasi K, Torabi M (2015) Effects of Artemisia annua extracts on sporulation of Eimeria oocysts. Parasitol Res 114:1207–1211

    Article  PubMed  Google Scholar 

  14. Gholamrezaie Sani L, Mohammadi M, JalaliSendi J, Abolghasemi SA, Roostaie Ali Mehr M (2013) Extract and leaf powder effect of Artemisia annua on performance, cellular and humoral immunity in broilers. IJVR 14:15–20

    Google Scholar 

  15. Goudarzi M, Rahbari S, Hadadzadeh H, Yeganehparsat M, Shafiyi A, Pourmaydani A (2005) Study the effect of leaf and extract of Artemisia annua on coccidiosis in broiler chickens. Vet Res 61:339–344

    Google Scholar 

  16. Jang SI, Jun MH, Lillehoj HS, Dallour RA, Kong IK, Kim S, Min W (2007) Anticoccidial effect of green tea-based diets against Eimeria maxima. Vet Parasitol 144:172–175

    CAS  Article  PubMed  Google Scholar 

  17. Kim Y, Wyslouzil BE, Weathers PJ (2001) A comparative study of mistand bubble column reactors in the in vitro production of artemisinin. Plant Cell Rep 20:451–455

    CAS  Article  Google Scholar 

  18. Kostadinovic L, Levic J, Galonja-Coghill T, Ruzicic L (2012) Anticoccidian effects of the Artemisia absinthium L. extracts in broiler chickens. Arch Zootec 15:69–77

    Google Scholar 

  19. Lapkin AA, Walker A, Sullivan N, Khambay B, Mlambo B, Chemat S (2009) Development of HPLC analytical protocols for quantification of artemisinin in biomass and extracts. Pharm Biomed Anal 49:908–915

    CAS  Article  Google Scholar 

  20. Mannan A, Ahmed I, Arshad W, Asim MF, Qureshi RA, Hussain I, Mirza B (2010) Survey of artemisinin production by diverse Artemisia species in northern Pakistan. Malar J 9:310–319

    Article  PubMed  PubMed Central  Google Scholar 

  21. McDougald LR (2013) Protozoal infections. In: Swayne DE (ed) Disease of poultry. Willey-Blackwell, USA, pp 1147–1201

    Google Scholar 

  22. Molan A, Liu Z, De S (2009) Effect of pine bark (Pinus radiata) extracts on sporulation of coccidian oocysts. Folia Parasitol 56:1–5

    Article  PubMed  Google Scholar 

  23. Muthamilselvan T, Kuo TF, Wu YC, Yang WC (2016) Herbal remedies for coccidiosis control: a review of plants. Comp Anticoccidial Actions 2016:1–19

    Google Scholar 

  24. National Research Council (1994) Nutrient requirements of poultry, 9th edn. National Academy Press, Washington D. C., pp 1–180

    Google Scholar 

  25. Oh HG, Youn HJ, Noh HW, Jang DH, Kang YB (1995) Anticoccidial effects of an extract of A. annua on Eimeria tenella. Vet Res 35:115–121

    Google Scholar 

  26. Shahbazfar A, Mardjanmehr SH, Arab HA, Rassouli A, Abdollahi M (2011) Effects of artemisinin in broiler chickens following chronic oral intake. Trop Anim Health Prod 43:843–849

    Article  PubMed  Google Scholar 

  27. Shahbazfar AA, Zare P, Mohammadpour H, Tayefi-Nasrabadi H (2012) Effects of different concentrations of artemisinin and artemisinin-iron combination treatment on Madin Darby canine kidney (MDCK) cells. Interdiscip Toxicol 1:30–37

    Google Scholar 

  28. Sprenger LK, Campestrini LH, Yamassaki FT, Buzatti A, Maurer JBB, Baggio SFZ, Melillo de Magalhães P, Molento MB (2015) Efeito anticoccidiano de extrato hidroalcoólico de Artemisia annua em camas de aves contaminadas com Eimeria sp. Pesq Vet Bras 35(7):649–651

    Article  Google Scholar 

  29. Towler MJ, Weathers PJ (2007) Evidence of artemisinin production from IPP stemming from both the mevalonate and the nonmevalonate pathways. Plant Cell Rep 26:2129–2136

    CAS  Article  PubMed  Google Scholar 

  30. World Health Organization (2006) WHO monograph on good agricultural and collection practices (GACP) for Artemisia annua L. WHO document :www.who.int/medicines/publications/traditional/Artemisia Monograph: 1–49 World Health Organization, Geneva

  31. World Health Organization (2011) Quality control methods for herbal materials. Updated edition of quality control methods for medicinal plant materials 1998. WHO document: www.who.int/medicinedocs/document: 1–187 World Health Organization, Geneva

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Acknowledgements

The authors wish to express their gratitude to Ghodratollah Rezaei and Hosein Darvishian for their technical assistance, Sobhan Firouzi for providing oocysts, Medicinal Plant Research Center of Barij, Kashan, Iran, especially Dr. Alireza Hatami, for helping in the collection and extraction of A. annua., and Agriculture and Natural Resources Research Center of Qom, Qom, Iran, especially Dr. Majid Torabi Goudarzi, for his consultation in herbal medicine.

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Correspondence to Ahmadreza Fatemi.

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Fatemi, A., Asasi, K. & Razavi, S.M. Anticoccidial effects of Artemisia annua ethanolic extract: prevention, simultaneous challenge-medication, and treatment. Parasitol Res 116, 2581–2589 (2017). https://doi.org/10.1007/s00436-017-5567-y

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Keywords

  • Artemisia annua
  • Ethanolic extract
  • Anticoccidial
  • Prevention