Abstract
Cuticular proteins (CPs) are implicated in insecticide resistance in mosquito populations. Here, we investigated the role of cuticular genes in regulation of insecticide resistance in Culex pipiens pallens. We identified two CpCPRs (CpCPR63 and CpCPR47) that exhibited higher transcript levels in pyrethroid-resistant strains than in susceptible strains. Mosquito mortality was increased after knockdown of CpCPR genes by dsRNA injection. The RNA interference experiment suggested an interaction between CpCPR63 and CpCPR47, as silencing of one gene resulted in decreased expression of the other. These findings revealed that CpCPRs may regulate pyrethroid resistance and could be used as a potential genetic marker to monitor pyrethroid resistance in mosquitoes.
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Acknowledgments
This work was supported by the National Institutes of Health of USA (NIH) [grant number 2R01AI075746], the National Natural Science Foundation of China [grant numbers 81672056, 81471984, 81171610, and 81301458], and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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Figure S1
a Expression levels of CPR63 at all stages of mosquito development. b Expression levels of CPR47 at all stages of mosquito development. The gene expression level at one-instar larva in Lab-DS strain was considered to be 1. The results are shown as the mean ± S.E. * p < 0.05, **p < 0.01 (JPEG 48.1 kb)
Figure S2
The mRNA levels of CpCPR48, CpCPR45 and CpCPR44 genes after injection with single and double targeting dsCPR. The mRNA levels of CpCPR48 decreased significantly (p < 0.05) at 72-h after dsCPR47 injection. The mRNA levels of the three CpCPRs was not statistically significant at 72-h after dsCPR63, dsCPR47 and dsCPRs injection. The results are shown as the mean ± S.E. * p < 0.05, **p < 0 .01 (JPEG 47.3 kb)
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Sun, X., Guo, J., Ye, W. et al. Cuticle genes CpCPR63 and CpCPR47 may confer resistance to deltamethrin in Culex pipiens pallens . Parasitol Res 116, 2175–2179 (2017). https://doi.org/10.1007/s00436-017-5521-z
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DOI: https://doi.org/10.1007/s00436-017-5521-z