Skip to main content

Advertisement

Log in

Molecular characterization of Blastocystis sp. in captive wild animals in Qinling Mountains

  • Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

A Correction to this article was published on 04 December 2017

This article has been updated

Abstract

Blastocystis is one common protist inhabiting in gastrointestinal tracts of animals and humans. Examining the subtypes has important implications for assessing the zoonotic potential of Blastocystis and intestinal health of hosts. In the present study, a total of 497 fecal samples collected from 37 wild animal species in Qinling Mountains were investigated for the presence and subtypes of Blastocystis. Of them, 200 (40.2%) were positive for Blastocystis and 13 subtypes were found, including eight known subtypes (STs1–3, 5, 10, 12–14) and five possible novel subtypes (temporarily named as STs18–22), with ST10 as the predominate subtype and the subtype ST5 was detected in an ostrich for the first time. These findings indicated the wide distribution and specific subtype characteristics of Blastocystis in wild animals of Qinling Mountains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Change history

  • 04 December 2017

    There were errors in Fig. 1 of the originally published article. Correct fig. 1 is presented here.

References

  • Abe N, Nagoshi M, Takami K, Sawano Y, Yoshikawa H (2002) A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan. Vet Parasitol 106:203–212

    Article  PubMed  Google Scholar 

  • Ajjampur SS, Tan KS (2016) Pathogenic mechanisms in Blastocystis spp.—interpreting results from in vitro and in vivo studies. Parasitol Int 65:772–779

    Article  CAS  PubMed  Google Scholar 

  • Alfellani MA, Jacob AS, Perea NO, Krecek RC, Taner-Mulla D, Verweij JJ, Levecke B, Tannich E, Clark CG, Stensvold CR (2013a) Diversity and distribution of Blastocystis sp. subtypes in non-human primates. Parasitology 140:966–971

    Article  CAS  PubMed  Google Scholar 

  • Alfellani MA, Stensvold CR, Vidal-Lapiedra A, Onuoha ES, Fagbenro-Beyioku AF, Clark CG (2013b) Variable geographic distribution of Blastocystis subtypes and its potential implications. Acta Trop 126:11–18

    Article  PubMed  Google Scholar 

  • Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG (2013c) Genetic diversity of Blastocystis in livestock and zoo animals. Protist 164:497–509

    Article  CAS  PubMed  Google Scholar 

  • Andersen LO, Stensvold CR (2016) Blastocystis in health and disease: are we moving from a clinical to a public health perspective? J Clin Microbiol 54:524–528

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Badparva E, Sadraee J, Kheirandish F (2015) Genetic diversity of Blastocystis isolated from cattle in Khorramabad, Iran. Jundishapur J Microbiol 8:e14810

    Article  PubMed  PubMed Central  Google Scholar 

  • Cirioni O, Giacometti A, Drenaggi D, Ancarani F, Scalise G (1999) Prevalence and clinical relevance of Blastocystis hominis in diverse patient cohorts. Eur J Epidemiol 15:389–393

    Article  CAS  PubMed  Google Scholar 

  • Clark CG, van der Giezen M, Alfellani MA, Stensvold CR (2013) Recent developments in Blastocystis research. Adv Parasitol 82:1–32

    Article  PubMed  Google Scholar 

  • Dogruman-Al F, Kustimurm S, Yoshikawa H, Tuncer C, Simsek Z, Tanyuksel M, Araz E, Boorom K (2009) Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara. Turkey. Mem Inst Oswaldo Cruz 104:724–727

    Article  CAS  PubMed  Google Scholar 

  • El Safadi D, Cian A, Nourrisson C, Pereira B, Morelle C, Bastien P, Bellanger AP, Botterel F, Candolfi E, Desoubeaux G, Lachaud L, Morio F, Pomares C, Rabodonirina M, Wawrzyniak I, Delbac F, Gantois N, Certad G, Delhaes L, Poirier P, Viscogliosi E (2016) Prevalence, risk factors for infection and subtype distribution of the intestinal parasite Blastocystis sp. from a large-scale multi-center study in France. BMC Infect Dis 16:451

    Article  PubMed  PubMed Central  Google Scholar 

  • Krogsgaard LR, Engsbro AL, Stensvold CR, Nielsen HV, Bytzer P (2015) The prevalence of intestinal parasites is not greater among individuals with irritable bowel syndrome: a population-based case-control study. Clin Gastroenterol Hepatol 13:507–513

    Article  PubMed  Google Scholar 

  • Lim YA, Ngui R, Shukri J, Rohela M, Mat Naim HR (2008) Intestinal parasites in various animals at a zoo in Malaysia. Vet Parasitol 157:154–159

    Article  CAS  PubMed  Google Scholar 

  • Nagel R, Bielefeldt-Ohmann H, Traub R (2014) Clinical pilot study: efficacy of triple antibiotic therapy in Blastocystis positive irritable bowel syndrome patients. Gut Pathog 6:34

    PubMed  PubMed Central  Google Scholar 

  • Navarro C, Domínguez-Márquez MV, Garijo-Toledo MM, Vega-García S, Fernández-Barredo S, Pérez-Gracia MT, García A, Borrás R, Gómez-Muñoz MT (2008) High prevalence of Blastocystis sp. in pigs reared under intensive growing systems: frequency of ribotypes and associated risk factors. Vet Parasitol 153:347–358

    Article  CAS  PubMed  Google Scholar 

  • Parkar U, Traub RJ, Vitali S, Elliot A, Levecke B, Robertson I, Geurden T, Steele J, Drake B, Thompson RC (2010) Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet Parasitol 169:8–17

    Article  CAS  PubMed  Google Scholar 

  • Petersen AM, Stensvold CR, Mirsepasi H, Engberg J, Friis-Møller A, Porsbo LJ, Hammerum AM, Nordgaard-Lassen I, Nielsen HV, Krogfelt KA (2013) Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection. Scand J Gastroenterol 48:638–639

    Article  PubMed  Google Scholar 

  • Poirier P, Wawrzyniak I, Albert A, El Alaoui H, Delbac F, Livrelli V (2011) Development and evaluation of a real-time PCR assay for detection and quantification of Blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. J Clin Microbiol 49:975–983

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ramírez JD, Sánchez LV, Bautista DC, Corredor AF, Flórez AC, Stensvold CR (2014) Blastocystis subtypes detected in humans and animals from Colombia. Infect Genet Evol 22:223–228

    Article  PubMed  Google Scholar 

  • Ramírez JD, Sánchez A, Hernández C, Flórez C, Bernal MC, Giraldo JC, Reyes P, López MC, García L, Cooper PJ, Vicuña Y, Mongi F, Casero RD (2016) Geographic distribution of human Blastocystis subtypes in South America. Infect Genet Evol 41:32–35

    Article  PubMed  Google Scholar 

  • Scanlan PD (2012) Blastocystis: past pitfalls and future perspectives. Trends Parasitol 28:327–334

    Article  PubMed  Google Scholar 

  • Scanlan PD, Stensvold CR, Rajilić-Stojanović M, Heilig HG, De Vos WM, O’Toole PW, Cotter PD (2014) The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. FEMS Microbiol Ecol 90:326–330

    Article  CAS  PubMed  Google Scholar 

  • Silberman JD, Sogin ML, Leipe DD, Clark CG (1996) Human parasite finds taxonomic home. Nature 380:398

    Article  CAS  PubMed  Google Scholar 

  • Stensvold CR, Clark CG (2016) Current status of Blastocystis: a personal view. Parasitol Int 65:763–771

    Article  PubMed  Google Scholar 

  • Stensvold CR, Alfellani MA, Nørskov-Lauritsen S, Prip K, Victory EL, Maddox C, Nielsen HV, Clark CG (2009a) Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. Int J Parasitol 39:473–479

    Article  CAS  PubMed  Google Scholar 

  • Stensvold CR, Nielsen HV, Molbak K, Smith HV (2009b) Pursuing the clinical significance of Blastocystis- diagnostic limitations. Trends Parasitol 25:23–29

    Article  PubMed  Google Scholar 

  • Stensvold CR, Smith HV, Nagel R, Olsen KE, Traub RJ (2010) Eradication of Blastocystis carriage with antimicrobials: reality or delusion? J Clin Gastroenterol 44:85–90

    Article  PubMed  Google Scholar 

  • Stensvold CR, Alfellani MA, Clark CG (2012) Levels of genetic diversity vary dramatically between Blastocystis subtypes. Infect Genet Evol 12:263–273

    Article  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tan KS (2004) Blastocystis in humans and animals: new insights using modern methodologies. Vet Parasitol 126:121–144

    Article  PubMed  Google Scholar 

  • Tan KS (2008) New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 21:639–665

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tsaousis AD, Ollagnier de Choudens S, Gentekaki E, Long S, Gaston D, Stechmann A, Vinella D, Py B, Fontecave M, Barras F, Lukeš J, Roger AJ (2012) Evolution of Fe/S cluster biogenesis in the anaerobic parasite Blastocystis. Proc Natl Acad Sci U S A 109:10426–10431

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Walderich B, Bernauer S, Renner M, Knobloch J, Burchard GD (1998) Cytopathic effects of Blastocystis hominis on Chinese hamster ovary (CHO) and adeno carcinoma HT29 cell cultures. Tropical Med Int Health 3:385–390

    Article  CAS  Google Scholar 

  • Wang W, Owen H, Traub RJ, Cuttell L, Inpankaew T, Bielefeldt-Ohmann H (2014) Molecular epidemiology of Blastocystis in pigs and their in-contact humans in Southeast Queensland, Australia, and Cambodia. Vet Parasitol 203:264–269

    Article  PubMed  Google Scholar 

  • Yan Y, Su S, Ye J, Lai X, Lai R, Liao H, Chen G, Zhang R, Hou Z, Luo X (2007) Blastocystis sp. subtype 5: a possibly zoonotic genotype. Parasitol Res 101:1527–1532

    Article  PubMed  Google Scholar 

  • Yoshikawa H, Abe N, Wu Z (2004) PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds. Microbiology 150:1147–1151

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to S. K. Yu or J. K. Song.

Additional information

A correction to this article is available online at https://doi.org/10.1007/s00436-017-5692-7.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, G.H., Hu, X.F., Liu, T.L. et al. Molecular characterization of Blastocystis sp. in captive wild animals in Qinling Mountains. Parasitol Res 116, 2327–2333 (2017). https://doi.org/10.1007/s00436-017-5506-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-017-5506-y

Keywords

Navigation