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Parasitology Research

, Volume 116, Issue 8, pp 2327–2333 | Cite as

Molecular characterization of Blastocystis sp. in captive wild animals in Qinling Mountains

  • G. H. Zhao
  • X. F. Hu
  • T. L. Liu
  • R. S. Hu
  • Z. Q. Yu
  • W. B. Yang
  • Y. L. Wu
  • S. K. Yu
  • J. K. Song
Short Communication

Abstract

Blastocystis is one common protist inhabiting in gastrointestinal tracts of animals and humans. Examining the subtypes has important implications for assessing the zoonotic potential of Blastocystis and intestinal health of hosts. In the present study, a total of 497 fecal samples collected from 37 wild animal species in Qinling Mountains were investigated for the presence and subtypes of Blastocystis. Of them, 200 (40.2%) were positive for Blastocystis and 13 subtypes were found, including eight known subtypes (STs1–3, 5, 10, 12–14) and five possible novel subtypes (temporarily named as STs18–22), with ST10 as the predominate subtype and the subtype ST5 was detected in an ostrich for the first time. These findings indicated the wide distribution and specific subtype characteristics of Blastocystis in wild animals of Qinling Mountains.

Keywords

Blastocystis Subtypes Wild animals Qinling Mountains 

References

  1. Abe N, Nagoshi M, Takami K, Sawano Y, Yoshikawa H (2002) A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan. Vet Parasitol 106:203–212CrossRefPubMedGoogle Scholar
  2. Ajjampur SS, Tan KS (2016) Pathogenic mechanisms in Blastocystis spp.—interpreting results from in vitro and in vivo studies. Parasitol Int 65:772–779CrossRefPubMedGoogle Scholar
  3. Alfellani MA, Jacob AS, Perea NO, Krecek RC, Taner-Mulla D, Verweij JJ, Levecke B, Tannich E, Clark CG, Stensvold CR (2013a) Diversity and distribution of Blastocystis sp. subtypes in non-human primates. Parasitology 140:966–971CrossRefPubMedGoogle Scholar
  4. Alfellani MA, Stensvold CR, Vidal-Lapiedra A, Onuoha ES, Fagbenro-Beyioku AF, Clark CG (2013b) Variable geographic distribution of Blastocystis subtypes and its potential implications. Acta Trop 126:11–18CrossRefPubMedGoogle Scholar
  5. Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG (2013c) Genetic diversity of Blastocystis in livestock and zoo animals. Protist 164:497–509CrossRefPubMedGoogle Scholar
  6. Andersen LO, Stensvold CR (2016) Blastocystis in health and disease: are we moving from a clinical to a public health perspective? J Clin Microbiol 54:524–528CrossRefPubMedPubMedCentralGoogle Scholar
  7. Badparva E, Sadraee J, Kheirandish F (2015) Genetic diversity of Blastocystis isolated from cattle in Khorramabad, Iran. Jundishapur J Microbiol 8:e14810CrossRefPubMedPubMedCentralGoogle Scholar
  8. Cirioni O, Giacometti A, Drenaggi D, Ancarani F, Scalise G (1999) Prevalence and clinical relevance of Blastocystis hominis in diverse patient cohorts. Eur J Epidemiol 15:389–393CrossRefPubMedGoogle Scholar
  9. Clark CG, van der Giezen M, Alfellani MA, Stensvold CR (2013) Recent developments in Blastocystis research. Adv Parasitol 82:1–32CrossRefPubMedGoogle Scholar
  10. Dogruman-Al F, Kustimurm S, Yoshikawa H, Tuncer C, Simsek Z, Tanyuksel M, Araz E, Boorom K (2009) Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara. Turkey. Mem Inst Oswaldo Cruz 104:724–727CrossRefPubMedGoogle Scholar
  11. El Safadi D, Cian A, Nourrisson C, Pereira B, Morelle C, Bastien P, Bellanger AP, Botterel F, Candolfi E, Desoubeaux G, Lachaud L, Morio F, Pomares C, Rabodonirina M, Wawrzyniak I, Delbac F, Gantois N, Certad G, Delhaes L, Poirier P, Viscogliosi E (2016) Prevalence, risk factors for infection and subtype distribution of the intestinal parasite Blastocystis sp. from a large-scale multi-center study in France. BMC Infect Dis 16:451CrossRefPubMedPubMedCentralGoogle Scholar
  12. Krogsgaard LR, Engsbro AL, Stensvold CR, Nielsen HV, Bytzer P (2015) The prevalence of intestinal parasites is not greater among individuals with irritable bowel syndrome: a population-based case-control study. Clin Gastroenterol Hepatol 13:507–513CrossRefPubMedGoogle Scholar
  13. Lim YA, Ngui R, Shukri J, Rohela M, Mat Naim HR (2008) Intestinal parasites in various animals at a zoo in Malaysia. Vet Parasitol 157:154–159CrossRefPubMedGoogle Scholar
  14. Nagel R, Bielefeldt-Ohmann H, Traub R (2014) Clinical pilot study: efficacy of triple antibiotic therapy in Blastocystis positive irritable bowel syndrome patients. Gut Pathog 6:34PubMedPubMedCentralGoogle Scholar
  15. Navarro C, Domínguez-Márquez MV, Garijo-Toledo MM, Vega-García S, Fernández-Barredo S, Pérez-Gracia MT, García A, Borrás R, Gómez-Muñoz MT (2008) High prevalence of Blastocystis sp. in pigs reared under intensive growing systems: frequency of ribotypes and associated risk factors. Vet Parasitol 153:347–358CrossRefPubMedGoogle Scholar
  16. Parkar U, Traub RJ, Vitali S, Elliot A, Levecke B, Robertson I, Geurden T, Steele J, Drake B, Thompson RC (2010) Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet Parasitol 169:8–17CrossRefPubMedGoogle Scholar
  17. Petersen AM, Stensvold CR, Mirsepasi H, Engberg J, Friis-Møller A, Porsbo LJ, Hammerum AM, Nordgaard-Lassen I, Nielsen HV, Krogfelt KA (2013) Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection. Scand J Gastroenterol 48:638–639CrossRefPubMedGoogle Scholar
  18. Poirier P, Wawrzyniak I, Albert A, El Alaoui H, Delbac F, Livrelli V (2011) Development and evaluation of a real-time PCR assay for detection and quantification of Blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. J Clin Microbiol 49:975–983CrossRefPubMedPubMedCentralGoogle Scholar
  19. Ramírez JD, Sánchez LV, Bautista DC, Corredor AF, Flórez AC, Stensvold CR (2014) Blastocystis subtypes detected in humans and animals from Colombia. Infect Genet Evol 22:223–228CrossRefPubMedGoogle Scholar
  20. Ramírez JD, Sánchez A, Hernández C, Flórez C, Bernal MC, Giraldo JC, Reyes P, López MC, García L, Cooper PJ, Vicuña Y, Mongi F, Casero RD (2016) Geographic distribution of human Blastocystis subtypes in South America. Infect Genet Evol 41:32–35CrossRefPubMedGoogle Scholar
  21. Scanlan PD (2012) Blastocystis: past pitfalls and future perspectives. Trends Parasitol 28:327–334CrossRefPubMedGoogle Scholar
  22. Scanlan PD, Stensvold CR, Rajilić-Stojanović M, Heilig HG, De Vos WM, O’Toole PW, Cotter PD (2014) The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. FEMS Microbiol Ecol 90:326–330CrossRefPubMedGoogle Scholar
  23. Silberman JD, Sogin ML, Leipe DD, Clark CG (1996) Human parasite finds taxonomic home. Nature 380:398CrossRefPubMedGoogle Scholar
  24. Stensvold CR, Clark CG (2016) Current status of Blastocystis: a personal view. Parasitol Int 65:763–771CrossRefPubMedGoogle Scholar
  25. Stensvold CR, Alfellani MA, Nørskov-Lauritsen S, Prip K, Victory EL, Maddox C, Nielsen HV, Clark CG (2009a) Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. Int J Parasitol 39:473–479CrossRefPubMedGoogle Scholar
  26. Stensvold CR, Nielsen HV, Molbak K, Smith HV (2009b) Pursuing the clinical significance of Blastocystis- diagnostic limitations. Trends Parasitol 25:23–29CrossRefPubMedGoogle Scholar
  27. Stensvold CR, Smith HV, Nagel R, Olsen KE, Traub RJ (2010) Eradication of Blastocystis carriage with antimicrobials: reality or delusion? J Clin Gastroenterol 44:85–90CrossRefPubMedGoogle Scholar
  28. Stensvold CR, Alfellani MA, Clark CG (2012) Levels of genetic diversity vary dramatically between Blastocystis subtypes. Infect Genet Evol 12:263–273CrossRefPubMedGoogle Scholar
  29. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  30. Tan KS (2004) Blastocystis in humans and animals: new insights using modern methodologies. Vet Parasitol 126:121–144CrossRefPubMedGoogle Scholar
  31. Tan KS (2008) New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 21:639–665CrossRefPubMedPubMedCentralGoogle Scholar
  32. Tsaousis AD, Ollagnier de Choudens S, Gentekaki E, Long S, Gaston D, Stechmann A, Vinella D, Py B, Fontecave M, Barras F, Lukeš J, Roger AJ (2012) Evolution of Fe/S cluster biogenesis in the anaerobic parasite Blastocystis. Proc Natl Acad Sci U S A 109:10426–10431CrossRefPubMedPubMedCentralGoogle Scholar
  33. Walderich B, Bernauer S, Renner M, Knobloch J, Burchard GD (1998) Cytopathic effects of Blastocystis hominis on Chinese hamster ovary (CHO) and adeno carcinoma HT29 cell cultures. Tropical Med Int Health 3:385–390CrossRefGoogle Scholar
  34. Wang W, Owen H, Traub RJ, Cuttell L, Inpankaew T, Bielefeldt-Ohmann H (2014) Molecular epidemiology of Blastocystis in pigs and their in-contact humans in Southeast Queensland, Australia, and Cambodia. Vet Parasitol 203:264–269CrossRefPubMedGoogle Scholar
  35. Yan Y, Su S, Ye J, Lai X, Lai R, Liao H, Chen G, Zhang R, Hou Z, Luo X (2007) Blastocystis sp. subtype 5: a possibly zoonotic genotype. Parasitol Res 101:1527–1532CrossRefPubMedGoogle Scholar
  36. Yoshikawa H, Abe N, Wu Z (2004) PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds. Microbiology 150:1147–1151CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • G. H. Zhao
    • 1
  • X. F. Hu
    • 1
  • T. L. Liu
    • 1
  • R. S. Hu
    • 1
  • Z. Q. Yu
    • 1
  • W. B. Yang
    • 1
  • Y. L. Wu
    • 2
  • S. K. Yu
    • 1
  • J. K. Song
    • 1
  1. 1.College of Veterinary MedicineNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Xian Qinling Wildlife ParkXianPeople’s Republic of China

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