Molecular studies with Aedes (Stegomyia) aegypti (Linnaeus, 1762), mosquito transmitting the dengue virus

Abstract

Dengue is an infectious viral disease, which can present a wide clinical picture, ranging from oligo or asymptomatic forms, to bleeding and shock, and can progress to death. The disease problem has increased in recent years, especially in urban and suburban areas of tropical and subtropical regions. There are five dengue viruses, called serotypes (DEN-1, DEN-2, DEN-3, DEN-4, and DEN-5), which belong to the Flaviviridae family and are transmitted to humans through infected mosquito bites, with the main vector the Aedes aegypti mosquito (Linnaeus, 1762). Studies performed with Ae. aegypti, aimed at their identification and analysis of their population structure, are fundamental to improve understanding of the epidemiology of dengue, as well for the definition of strategic actions that reduce the transmission of this disease. Therefore, considering the importance of such research to the development of programs to combat dengue, the present review considers the techniques used for the molecular identification, and evaluation of the genetic variability of Ae. aegypti.

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References

  1. Apostol BL, Black WC IV, Reiter P, Miller BR (1996) Population genetics with RAPD—PCR markers: the breeding structure of Aedes aegypti in Puerto Rico. Heredity 76:325–334

    CAS  Article  PubMed  Google Scholar 

  2. Ayres CFJ, Melo-Santos MAV, Solé-Cava AM, Furtado AF (2003) Genetic differentiation of Aedes aegypti (Diptera: Culicidae), the major dengue vector in Brazil. J Med Entomol 40:430–435. doi:10.1603/0022-2585-40.4.430

    CAS  Article  PubMed  Google Scholar 

  3. Barata EMF, Costa AIP, Neto FC, Glasser CM, Barata JM, Natal D (2001) População de Aedes aegypti (l.) em área endêmica de dengue, Sudeste do Brasil. Rev Saúde Pública 35(3):237–242

    CAS  Article  PubMed  Google Scholar 

  4. Barreto ML, Teixeira MG (2008) Dengue no Brasil: situação epidemiológica e contribuições para uma agenda de pesquisa. Estudos Avançados 64:22

    Google Scholar 

  5. Beebe NW, Whelan PI, Hurk AVD, Ritchie SA, Cooper RD (2005) Genetic diversity of the dengue vector Aedes aegypti in Australia and implications for future surveillance and mainland incursion monitoring. CDI 29:299–304

    PubMed  Google Scholar 

  6. Bosio CF, Harrington LC, Jones JW, Sithiprasasna R, Norris DE, Scott TW (2005) Genetic structure of Aedes aegypti populations in Thailand using mitochondrial DNA. AmJTrop Med Hyg 72:434–442

    CAS  Google Scholar 

  7. Bracco JE, Capurro ML, Lourenço-de-Oliveira R, Sallum MAM (2007) Genetic variability of Aedes aegypti in the Americas using a mitochondrial gene: evidence of multiple introductions. Mem Inst Oswaldo Cruz 102:573–580

    CAS  Article  PubMed  Google Scholar 

  8. Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde (2005) Guia de vigilância epidemiológica/Ministério da Saúde, Secretaria de Vigilância em Saúde. – 6. ed. – Brasília: Ministério da Saúde.

  9. Brasil. Ministério da Saúde – Descrição da doença. http://portalsaude.saude.gov.br/index.php/descricao-da-doenca-dengue. Accessed 03 november 2016.

  10. Bronzato AR (2015) Análise da estrutura populacional de Aedes aegytpi (Linnaeus, 1762) em algumas regiões do Brasil. Dissertação, Universidade Estadual Paulista.

  11. Chan A, Chiang LP, Hapuarachchi HC, Tan CH, Pang SC, Lee R, Lee KS, Ching L, Lam-Phua SG (2014) DNA barcoding: complementing morphological identification of mosquito species in Singapore. Parasit & Vectors 7:1–12

    Article  Google Scholar 

  12. Das B, Swain S, Patra A, Das M, Tripathy HK, Mohapatra N, Kar SK, Hazra RK (2012) Development and evaluation of a single-step multiplex PCR to differentiate the aquatic stages of morphologically similar Aedes (subgenus: Stegomyia) species. Trop Med and Int Health 17(2):235–243. doi:10.1111/j.1365-3156.2011.02899

    CAS  Article  Google Scholar 

  13. Dias CN, Alves LPL, Rodrigues KAF, Brito MCA, Rosa CS, Amaral FMM, Monteiro OS, Andrade EHA, Maia JGS, Moraes DFC (2015) Chemical composition and larvicidal activity of essential oils extracted from Brazilian Legal Amazon plants against Aedes aegypti L. (Diptera: Culicidae). Evidance-Based Complement Alternative Medicine:1–8 dx.doi.org/10.1155/2015/490765

  14. Ebi KL, Nealon J (2016) Dengue in a changing climate. Environ Res 151:115–123. doi:10.1016/j.envres.2016.07.026

  15. Forattini OP. 2002 Culicidologia Médica: Identificação, Biologia, Epidemiologia. São Paulo: Edusp; . v.2.

  16. Fraga EC, Santos JMM, Maia JF (2003) Enzymatic variability in Aedes aegypti (Diptera: Culicidae) populations from Manaus-AM, Brazil. Genet Mol Biol 26:181–187

    CAS  Article  Google Scholar 

  17. Freitas MTS, Gomes-Júnior PP, Batista MVA, Leal-Balbino TC, Araujo AL, Balbino VQ (2014) Novel DNA extraction assay for molecular identification of Aedes spp eggs. Genet Mol Res 13:8776–8782

    CAS  Article  PubMed  Google Scholar 

  18. Gadelha DP, Toda AT (1985) Biologia e comportamento do Aedes aegypti. Rev Brasl Malariol D Trop 37:29–36

    CAS  Google Scholar 

  19. García-Franco F, Munõz ML, Lozano-Fuentes S, Fernandez-Salas I, Garcia-Rejon J, Beaty BJ, Black WC IV (2002) Large genetic distances among Aedes aegypti populations along the south pacific coast of Mexico. AmJTrop Med Hyg 6:594–598

    Article  Google Scholar 

  20. Guissoni ACP, Silva IG, Geris R, Cunha LC, Silva HHG (2013) Atividade larvicida de Anacardium occidentale como alternativa ao controle de Aedes aegypti e sua toxicidade em Rattus norvegicus. Rev Bras Plantas Med 15:3

    Article  Google Scholar 

  21. Gupta K, Dhawan R, Kajla M, Kumar S, Jnanasiddhy B, Singh NK, Dixit R, Bihani A, Gupta L (2010) Molecular identification of Aedes aegypti mosquitoes from Pilani region of Rajasthan, India. Jpn J Infect Dis 63:312–316

    Google Scholar 

  22. Guzman MG, Harris E (2014) Dengue. Lancet 385:453–465

  23. Higa Y, Toma T, Tsuda Y, Miyagi IA (2010) Multiplex PCR-based molecular identification of five morphologically related, medically important subgenus Stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu Archipelago, Japan. Jpn J Infect Dis 63:312–316

    CAS  PubMed  Google Scholar 

  24. Hiragi C, Simões K, Martins E, Queiroz P, Lima L, Monnerat R (2009) Variabilidade genética em populações de Aedes aegypti (L.) (Diptera: Culicidae) utilizando marcadores de RAPD. Neotrop Entomol 38:542–547

    CAS  Article  PubMed  Google Scholar 

  25. Hoffmann LV, Barroso PAV (2006) Marcadores moleculares como ferramentas para estudos de genética de plantas. Embrapa, Campina Grande.

  26. Julio NB, Chiappero MB, Rossi HJ, Dueñas JCR, Gardenal CN (2009) Genetic structure of Aedes aegypti in the city of Córdoba (Argentina), a recently reinfested area. Mem Inst Oswaldo Cruz 104:626–631

    CAS  Article  PubMed  Google Scholar 

  27. Júnior FPC (2010) Ciclos de vidas comparados e variabilidade genética de Aedes aegypti (Diptera: Culicidae) do semi-árido paraibano. Dissertação, Universidade Estadual da Paraíba.

  28. Lacerda DR, Acedo MDP, Lemos Filho JP, Lovato MB (2002) A técnica de RAPD: uma ferramenta molecular em estudos de conservação de plantas. Lundiana 3:87–92

    Google Scholar 

  29. Lourenço-de-Oliveira R, Vazeille M, Filippis AMB, Failloux AB (2004) Aedes aegypti in Brazil: genetically differentiated populations with high susceptibility to dengue and yellow fever viroses. T Roy Soc Trop Med H 98:43–54

    Article  Google Scholar 

  30. Matsui QYP (2013) Sequenciamento parcial do gene codificador da cromo-helicase ligadora de DNA (CHD-1) de Mimus saturninus (Lichtenstein, 1823) e análise in sílico do gene da subunidade 1 da citocromo oxidase (COI) em Mimidae (Bonaparte, 1853). Dissertação, Universidade do Vale do Paraíba.

  31. Mendonça BAA, Sousa ACB, Souza AP, Scarpassa VM (2014) Temporal genetic structure of major dengue vector Aedes aegypti from Manaus, Amazonas, Brazil. Acta Trop 134:80–88

    Article  PubMed  Google Scholar 

  32. Muller GA, Marcondes CB, Navarro-Silva MA (2010) Aplicação de marcadores microssatélites para o estudo de Culicidae (Diptera): revisão com especial referência a Haemagogus. Bol Mal Salud Amb L:175-186.

  33. Murray NEA, Quam MB, Wilder-Smith A (2013) Epidemiology of dengue: past, present and future prospects. Clin Epidemiol 5:299–309. doi:10.2147/CLEP.S34440

  34. Mustafa LtCol MS, Rasotgi Col V, Jain Col S, LtCol G (2015) Discovery of fifth serotype of dengue virus (DENV-5): a new public health dilemma in dengue control. Medical journal armed forces índia voloume 71:67–70

    Article  Google Scholar 

  35. Oliveira JA, Crispim BA, Martins NM, Silva AO, Dourado PLR, Rocha MP, Grisolia AB (2013a) Sequências de gene mitocondrial para identificação de espécies animais. Rev Colombiana Cienc Anim 5:396–407

    Google Scholar 

  36. Oliveira VS, Pimenteira C, Silva-Alves DCB, Leal LLL, RAW N-F, DMAF N, Geanne KN, Santos KA, JVA D, Thereza AS (2013b) The enzyme 3-hydroxykynurenine transaminase as potential target for 1,2,4-oxadiazoles with larvicide activity against the dengue vector Aedes aegypti. Bioorganic & Medicinal Chem 21(22):6996–7003

    CAS  Article  Google Scholar 

  37. Olson KE, Allen-Miura T, Rayms-Keller A, Carlson JO, Coates C, Jasinskiene N, James A, Beaty B, Higgs S, Olson KE (1999) Inhibition of luciferase expression in transgenic Aedes aegypti mosquitoes by Sindbis virus expression of antisense luciferase RNA. Proc of the Nat Acad of Scien 96:13399–13403

    Article  Google Scholar 

  38. Paduan KS, Ribolla PEM (2008) Mitochondrial DNA polymorphism and heteroplasmy in populations of Aedes aegypti in Brazil. J Med Entomol 45:59–67. doi:10.1603/00222585%282008%2945%5B59%3AMDPAHI%5D2.0.CO%3B2

    Article  Google Scholar 

  39. Paduan KS, Araújo-Júnior JP, Ribolla PEM (2006) Genetic variability in geographical populations of Aedes aegypti (Diptera, Culicidae) in Brazil elucidated by molecular markers. Genet Mol Biol 29:391–395

    CAS  Article  Google Scholar 

  40. Paneto GG (2006) Utilização do DNA mitocondrial no contexto forense brasileiro. Dissertação, Universidade Estadual Paulista.

  41. Patarro TF (2010) Estudo da variabilidade genética de populações de Aedes aegypti (Diptera, Culicidae), resistentes e suscetíveis a inseticidas. Dissertação, Universidade Estadual Paulista.

  42. Saengwiman S, Aroonkesorn A, Dedvisitsakul P, Sakdee S, Leetachewa S, Angsuthanasombat C, Pootanak K (2011) In vivo identification of Bacillus thuringiensis Cry4Ba toxin receptors by RNA interference knockdown of glycosylphosphatidylinositol-linked aminopeptidase N transcripts in Aedes aegypti larvae. Bioch Biophys Res Commun 407:4:708–713

    Article  Google Scholar 

  43. Santos VM, MLG M, MTM A, Avila PE, Kirchgatter K (2003) Analysis of genetic relatedness between populations of Aedes aegypti from different geographic regions of São Paulo state, Brazil. Rev Inst Med trop S Paulo 45:99–101

    Article  PubMed  Google Scholar 

  44. Sayson SL, Gloria-Soria A, Powell JR, Edillo FE (2015) Seasonal genetic changes of Aedes aegypti (Diptera: Culicidae) populations in selected sites of Cebu City, Philippines. J Med Entomol 52:638–646. doi:10.1093/jme/tjv056

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  45. Silva RG, Pinhati FR, Silva JT (2015) Análise da variabilidade genética por RAPD de linhagens isoladas de solo e lodo impactados com efluente industrial. Revista da Biologia 14:1–5. doi:10.7594/revbio.14.01.01

    CAS  Article  Google Scholar 

  46. Soares TS, Watanabe RMO, Lemos FJA, Tanaka AS (2011) Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegyptilarvae and identification of digestive enzymes. Gene 489(2):70-75

  47. Sousa GB, Blanco A, Gardenal CM (2001) Genetic relationships among Aedes aegypti (Diptera: Culicidae) populations from Argentina using random amplified polymorphic DNA polymerase chain reaction markers. J Med Entomol 38:371–375. doi:10.1603/0022-2585-38.3.371

    Article  PubMed  Google Scholar 

  48. Souza KR (2011) Avaliação de parâmetros moleculares para vigilância entomológica do Aedes (Stegomyia) aegypti (Linnaeus, 1762). Dissertação, Fundação Oswaldo Cruz.

  49. Spenassatto C (2011) Desenvolvimento de metodologia high-throughput para estudo populacional do mosquito Aedes aegypti e comparação de dados de genes nucleares com dados de genes mitocondriais. Dissertação, Universidade Estadual Paulista Júlio de Mesquita Filho.

  50. Sukonthabhirom S, Saengtharatip S, Jirakanchanakit N, Rongnoparut P, Yoksan S, Daorai A, Chareonviriyaphap T (2009) Genetic structure among Thai populations of Aedes aegypti mosquitoes. J Vector Ecol 34:43–49. doi:10.3376/038.034.0106

    Article  PubMed  Google Scholar 

  51. Suresh U, Murugan K, Benelli G, Marcello N, Barnard DR, Chellasamy PP, Kumar M, Subramaniam J, Devakumar D, Chandramohan B (2015) Tackling the growing threat of dengue: Phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector Aedes aegypti (Diptera: Culicidae). Parasitol Res 114:1551–1562

    Article  PubMed  Google Scholar 

  52. Tien TK, Vazeille-Falcoz M, Mousson L, Huong TH, Rodhain F, Huong NT, Failloux A (1999) Aedes aegypti in Ho Chi Minh City (Viet Nam): susceptibility to dengue 2 virus and genetic differentiation. T Roy Soc Trop Med H 93:581–586

    Article  Google Scholar 

  53. Travanty EA, Adelman ZN, Franz AWE, Keene KM, Beaty BJ, Blair CD, James AA, Olson KE (2004) Using RNA interference to develop dengue virus resistance in genetically modified Aedes aegypti. Insect Biochem Mol Bio Oxford 34:607–613

    CAS  Article  Google Scholar 

  54. World Health Organization and World Meteorological Organization (2012) Atlas of health and climate. Geneva, Switzerland:World Health Organization and World Meteorological Organization. p. 21.

  55. Zara ALS, Santos ASM, Fernandes-Oliveira ES, Carvalho RG, Coelho GE (2016) Estratégias de controle do Aedes aegypti: uma revisão. Epidemiol Serv Saude 25:391–404

    PubMed  Google Scholar 

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Correspondence to Luciana Patrícia Lima Alves Pereira.

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Pereira, L.P.L.A., Brito, M.C.A., Araruna, F.B. et al. Molecular studies with Aedes (Stegomyia) aegypti (Linnaeus, 1762), mosquito transmitting the dengue virus. Parasitol Res 116, 2057–2063 (2017). https://doi.org/10.1007/s00436-017-5484-0

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Keywords

  • Aedes aegypti
  • Dengue
  • Molecular biology
  • Molecular identification
  • Genetic variability