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Redescription of Chloromyxum ellipticum Li & Nie, 1973 (Myxosporea: Chloromyxidae) infecting the gall bladder of grass carp Ctenopharyngodon idellus Valenciennes, 1844, supplemented by morphological and molecular characteristics

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Abstract

The traditional taxonomy of the genus Chloromyxum Mingazzini, 1890 has been intensively challenged to be paraphyletic by recent ribosomal DNA (rDNA)-based phylogenetic analysis. Undersampling to get rich sequence data to infer more scientific phylogenetic relationships makes scientists conservatively assign all non-marine elasmobranch-infecting species as Chloromyxum sensu lato. Although complex ridge pattern on the spore surface observed by scanning electron microscopy was thought to be critical for the identification of Chloromyxum species, insufficient data also prevent this ultrastructural data to be a valid taxonomic feature for this genus. It is especial for Chloromyxum species to be reported in China. Molecular and ultrastructural characteristics are yet available for all 22 Chloromyxum species recorded in China. During the investigation of the diversity of coelozoic fish myxosporeans, Chloromyxum ellipticum Li & Nie, 1973 was found to highly infect the gall bladder of Ctenopharyngodon idellus Valenciennes, 1844 in Poyang Lake watershed of Jiangxi province, Eastern China. Here, we redescribed it by the currently recommended holistic approach of combining morphological, ultrastructural, and molecular characteristics. Mature spores were found floating free in the gall bladder, but no plasmodium observed. Spores are typical freshwater teleost-infecting Chloromyxum species, spherical or subspherical in lateral view, measuring 7.7 ± 0.08 μm (6.9–9.1) in length, 6.3 ± 0.09 μm (5.6–7.6) in width, and 5.8 ± 0.20 μm (5.2–6.3) in thickness. Four pyriform polar capsules, located at the anterior end of the spores, were equal in size, 3.3 ± 0.06 μm (2.2–4.1) long and 2.1 ± 0.03 μm (1.7–2.5) wide. Polar filaments coiled with four to five turns. Two equal spore valves are symmetrical, with 10–16 surface extrasutural ridges per valve, aligned along the longitudinal axis. The obtained partial 18S rDNA of C. ellipticum did not match any sequences available in GenBank. Phylogenetic analysis showed that C. ellipticum clustered firstly with Chloromyxum legeri with robust nodal support and grouped then with urinary system of freshwater teleost-infecting Chloromyxum clade, rather than other gall bladder of freshwater teleost-infecting clade.

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Acknowledgements

We appreciated much the help of Mr. DW Zhang of Administration Bureau of Liaofang Reservoir, Jiangxi Province, for collecting the fish specimens. The present work was financially supported by the Natural Sciences Foundation of China (31472296), Jiangsu Fishery Project (D2015-11), Basic project SB Russian Academy of Science (VI.51.1.3), and Russian Fund of Basic Research (14-04-91176 GFEN_a).

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Authors and Affiliations

  1. Fish Diseases Laboratory, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, People’s Republic of China

    J. Y. Zhang, Y. L. Zhao, D. Luo, Z. F. Xing, Q. Q. Zhang & X. H. Liu

  2. Institute of General and Experimental Biology of Siberian Branch RAS, Ulan-Ude, Russia

    M.D. Batueva

  3. University of Chinese Academy of Science, Beijing, 10049, China

    J. Y. Zhang, Y. L. Zhao, D. Luo & X. H. Liu

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  1. J. Y. Zhang
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  2. Y. L. Zhao
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  3. M.D. Batueva
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  4. D. Luo
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  5. Z. F. Xing
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  6. Q. Q. Zhang
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Correspondence to J. Y. Zhang or X. H. Liu.

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Zhang, J.Y., Zhao, Y.L., Batueva, M. et al. Redescription of Chloromyxum ellipticum Li & Nie, 1973 (Myxosporea: Chloromyxidae) infecting the gall bladder of grass carp Ctenopharyngodon idellus Valenciennes, 1844, supplemented by morphological and molecular characteristics. Parasitol Res 116, 1479–1486 (2017). https://doi.org/10.1007/s00436-017-5424-z

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  • DOI: https://doi.org/10.1007/s00436-017-5424-z

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