Parasitology Research

, Volume 116, Issue 2, pp 773–780 | Cite as

Description and phylogeny of a new microsporidium from the elm leaf beetle, Xanthogaleruca luteola Muller, 1766 (Coleoptera: Chrysomelidae)

  • Çağrı BekircanEmail author
  • Ufuk Bülbül
  • Halil İ. Güler
  • James J. Becnel
Original Paper


This study describes a new genus and species of microsporidia which is a pathogen of the elm leaf beetle, Xanthogaleruca luteola Muller, 1776 (Coleoptera: Chrysomelidae). The beetles were collected from Istanbul in Turkey. All developmental stages are uninucleate and in direct contact with the host cell cytoplasm. Giemsa-stained mature spores are oval in shape and measured 3.40 ± 0.37 μm in length and 1.63 ± 0.20 μm in width. These uninucleate spores have an isofilar polar filament with 11 turns. The spore wall was trilaminar (75 to 115 nm) with a rugose, electron-dense exospore (34 to 45 nm) and a thickened, electron-lucent endospore (65 to 80 nm) overlaying the plasmalemma. Morphological, ultrastructural, and molecular features indicate that the described microsporidium is dissimilar to all known microsporidian taxa and confirm that it has different taxonomic characters than other microsporidia infecting X. luteola and is named here as Rugispora istanbulensis n. gen., n. sp.


Coleoptera Elm Microsporidia Pathogen Ultrastructure 



The TEM analysis of this study was carried out in Eskişehir Osmangazi University (ESOGU) Central Research Laboratory, Research and Application Centre (ARUM). We are thankful to Assoc. Prof. İlknur Dağ and her team for making this study possible and her valuable comments.


  1. Andreadis TG, Hanula JL (1987) Ultrastructural study and description of Ovavesicula popilliae N. G., N. Sp. (Microsporidia: Pleistophoridae) from the Japanese beetle, Popillia japonica (Coleoptera: Scarabaeidae). J Protozool 34:15–21. doi: 10.1111/j.1550-7408.1987.tb03123.x CrossRefGoogle Scholar
  2. Balbiani EG (1882) Sur les microsporidies ou psorospermies des Articulés. C R Acad Sci Paris Ser D 95:1168–1171Google Scholar
  3. Becnel JJ, Andreadis TG (2014) Microsporidia in Insects. In: Microsporidia: pathogens of opportunity. 1st ed. John Wiley & Sons, Inc., pp 521–570.Google Scholar
  4. Becnel JJ, Jeyaprakash A, Hoy MA, Shapiro A (2002) Morphological and molecular characterization of a new microsporidian species from the predatory mite Metaseiulus occidentalis (Nesbitt) (Acari, Phytoseiidae). J Invertebr Pathol 79:163–172. doi: 10.1016/S0022-2011(02)00032-0 CrossRefPubMedGoogle Scholar
  5. Becnel JJ, Takvorian PM, Cali A (2014) Checklist of available generic names for microsporidia with type species and type hosts. In: Weiss LM, Becnel JJ (eds) Microsporidia: pathogens of opportunity. Wiley-Blackwell, USA, pp 671–687Google Scholar
  6. Boucias DG, Pendland JC (1998) Phylum Microsporidia. In: Principles of insect pathology. Springer Science + Business Media, Llc, New York, pp 399–414CrossRefGoogle Scholar
  7. Brooks WM, Becnel JJ, Kennedy GG (1988) Establishment of Endoreticulatus n. g. for Pleistophora fidelis (Hostounsky and Weiser, 1975) (Microsporidia: Pleistophoridae) based on the ultrastructure of a microsporidium in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). J Protozool 35:481–488CrossRefGoogle Scholar
  8. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  9. Hajek AE, Delalibera JI (2010) Fungal pathogens as classical biological control agents against arthropods. BioControl 55:147–158. doi: 10.1007/s10526-009-9253-6 CrossRefGoogle Scholar
  10. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  11. Hylis M, Weiser J, Oborník M, Vávra J (2005) DNA isolation from museum and type collection slides of microsporidia. J Invertebr Pathol 88:257–260. doi: 10.1016/j.jip.2005.02.004 CrossRefPubMedGoogle Scholar
  12. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120CrossRefPubMedGoogle Scholar
  13. Larsson JIR (2014) The primitive microsporidia. In: Microsporidia: pathogens of opportunity. First ed. (Eds: Weiss L.M. and Becnel J.J.). John Wiley & Sons, Inc., pp. 605–635Google Scholar
  14. O’Mahony EM, Tay WT, Paxton RJ (2007) Multiple rRNA variants in a single spore of the microsporidian Nosema bombi. J Eukaryot Microbiol 54:103–109. doi: 10.1111/j.1550-7408.2006.00232.x CrossRefPubMedGoogle Scholar
  15. Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212. doi: 10.1083/jcb.17.1.208 CrossRefPubMedPubMedCentralGoogle Scholar
  16. Solter LF, Becnel JJ, David HO (2012) Microsporidian Entomopathogens. In: Vega, F.E., Kaya, H.K. (Eds.), Insect Pathology. Elsevier Inc., pp. 221–263Google Scholar
  17. Sprague V, Becnel JJ, Hazard EI (1992) Taxonomy of phylum Microspora. Crit Rev Microbiol 18:285–395CrossRefPubMedGoogle Scholar
  18. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. Clin Microbiol Res 3:197–218Google Scholar
  19. Toguebaye BS, Bouix G (1989) Nosema galerucellae n. sp. Microsporidian (Protozoa, Microspora), parasite of Galerucella luteola Müller (Chrysomelidae, Coleoptera): development cycle and ultrastructure. Eur J Protistol 24:346–353. doi: 10.1016/S0932-4739(89)80005-7 CrossRefPubMedGoogle Scholar
  20. Undeen AH, Vávra J (1997) Research methods for entomopathogenic protozoa. In: Lacey L (ed) Manual of techniques in insect pathology. Biological techniques series. Academic Press, London, pp 117–151CrossRefGoogle Scholar
  21. Vossbrinck CR, Debrunner-Vossbrinck BA (2005) Molecular phylogeny of the microsporidia: ecological, ultrastructural and taxonomic considerations. Folia Parasitol 52:131–142. doi: 10.14411/fp.2005.017 CrossRefPubMedGoogle Scholar
  22. Vossbrinck CR, Maddox JV, Frideman S, Debrunner-Vossbrinck BA, Woese CR (1987) Ribosomal RNA sequence suggests microsporidia are extremely ancient eukaryotes. Nature 326:411–414. doi: 10.1038/326411a0 CrossRefPubMedGoogle Scholar
  23. Vossbrinck CR, Baker MD, Didier ES, Debrunner-Vossbrinck BA, Shadduck JA (1993) Ribosomal DNA sequences of Encephalitozoon hellem and Encephalitozoon cuniculi: species identification and phylogenetic construction. J Eukaryot Micorbiol 40:354–362. doi: 10.1111/j.1550-7408.1993.tb04928.x CrossRefGoogle Scholar
  24. Wang Y, Liu W, Jiang Y, Huang L, Irfan M, Shi S, Yang R, Qin L (2015) Morphological and molecular characterization of Nosema pernyi a microsporidian parasite in Antheraea pernyi. Parasitol Res 114:3327–3336. doi: 10.1007/s00436-015-4558-0 CrossRefPubMedGoogle Scholar
  25. Weiser J, Wegensteiner R, Zizka Z (1995) Canningia spinidentis gen. et sp. n. (Protista: Microspora), a new pathogen of the fir bark beetle Pityokteines spinidens. Folia Parasitol 42:1–10Google Scholar
  26. Wu Z, Jamieson S, Kielbaso J (1991) Urban forest pest management. J Arbor 17:150–158Google Scholar
  27. Yaman M, Bekircan Ç, Radek R, Linde A (2014) Nosema pieriae sp. n. (Microsporida, Nosematidae): a new microsporidian pathogen of the Cabbage Butterfly Pieris brassicae L. (Lepidoptera: Pieridae). Acta Protozool 53:223–232. doi: 10.4467/16890027AP.14.019.1600 Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Çağrı Bekircan
    • 1
    Email author
  • Ufuk Bülbül
    • 1
  • Halil İ. Güler
    • 2
  • James J. Becnel
    • 3
  1. 1.Department of Biology, Faculty of SciencesKaradeniz Technical UniversityTrabzonTurkey
  2. 2.Department of Molecular Biology and Genetic, Faculty of SciencesKaradeniz Technical UniversityTrabzonTurkey
  3. 3.United States Department of Agriculture, Agriculture Research ServiceCenter for Medical, Agricultural and Veterinary Entomology (CMAVE)GainesvilleUSA

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