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Proteomic analysis of Fasciola hepatica excretory and secretory products (FhESPs) involved in interacting with host PBMCs and cytokines by shotgun LC-MS/MS

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Abstract

Fasciola hepatica is a helminth parasite with a worldwide distribution, which can cause chronic liver disease, fasciolosis, leading to economic losses in the livestock and public health in many countries. Control is mostly reliant on the use of drugs, and as a result, drug resistance has now emerged. The identification of F. hepatica genes involved in interaction between the parasite and host immune system is utmost important to elucidate the evasion mechanisms of the parasite and develop more effective strategies against fasciolosis. In this study, we aimed to identify molecules in F. hepatica excretory and secretory products (FhESPs) interacting with the host peripheral blood mononuclear cells (PBMCs), Th1-like cytokines (IL2 and IFN-γ), and Th17-like cytokines (IL17) by Co-IP combined with tandem mass spectrometry. The results showed that 14, 16, and 9 proteins in FhESPs could bind with IL2, IL17, and IFN-γ, respectively, which indicated that adult F. hepatica may evade the host immune responses through directly interplaying with cytokines. In addition, nine proteins in FhESPs could adhere to PBMCs. Our findings provided potential targets as immuno-regulators, and will be helpful to elucidate the molecular basis of host–parasite interactions and search for new potential proteins as vaccine and drug target candidates.

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Acknowledgments

Project support was provided by the “National Key Basic Research Program (973 Program) of China” (Grant no. 2015CB150300). The authors thank Shanghai Applied Protein Technology Co. Ltd., for technical assistance in LC-MS/MS analysis.

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Authors and Affiliations

  1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, People’s Republic of China

    Qing Liu, Si-Yang Huang, Dong-Mei Yue, Jin-Lei Wang & Xing-Quan Zhu

  2. College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, 410128, People’s Republic of China

    Qing Liu

  3. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Yujian Wang & Xiangrui Li

  4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, 225009, People’s Republic of China

    Si-Yang Huang & Xing-Quan Zhu

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  1. Qing Liu
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  2. Si-Yang Huang
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  3. Dong-Mei Yue
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  4. Jin-Lei Wang
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  5. Yujian Wang
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Correspondence to Si-Yang Huang.

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Liu, Q., Huang, SY., Yue, DM. et al. Proteomic analysis of Fasciola hepatica excretory and secretory products (FhESPs) involved in interacting with host PBMCs and cytokines by shotgun LC-MS/MS. Parasitol Res 116, 627–635 (2017). https://doi.org/10.1007/s00436-016-5327-4

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  • DOI: https://doi.org/10.1007/s00436-016-5327-4

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