Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014–2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 °C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Anonymous (2014) Boletín integrado de vigilancia. Ministerio de Salud de la República Argentina. Presidencia de la Nación. http://www.msal.gov.ar/images/stories/boletines/Boletin%20Integrado%20De%20Vigilancia%20N200-SE52.pdf. Accesed 11 June 2016
Anonymous (2015a) Servicio Meteorológico Nacional. http://www.smn.gov.ar/. Accesed 11 June 2016
Anonymous (2015b) Servicios Climáticos. Servicio Meteorológico Nacional. http://www.smn.gov.ar/serviciosclimaticos/clima/archivo/verano.pdf. Accesed 10 June 2016
Dzik JM, Gołos B, Jagielska E, Kapała A, Wałajtys-Rode E (2002) Early response of guinea-pig lungs to Trichinella spiralis infection. Parasite Immunol 24(7):369–379
Fariña F, Scialfa E, Bolpe J, Pasqualetti M, Rosa A, Ribicich M (2012) Study of Trichinella spp. in rodents that live near pig farms in an endemic region of the province of Buenos Aires, Argentina. J Bacteriol Parasitol 3:140
Fariña FA, Pasqualetti MI, Cardillo N, Aronowicz T, Ercole M, Krivokapich SJ, Ribicich M (2016) Evaluación de la transmisión galactógena de Trichinella patagoniensis en ratones BALB/c. Rev Argent Microbiol 48(2):101–104
Gamble HR, Bessonov AS, Cuperlovic K, Gajadhar AA, Van Knapen F, Noeckler K, Schenone H, Zhu X (2000) International commission on trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild animals intended for human consumption. Vet Parasitol 93:393–408
Gottstein B, Pozio E, Nockler K (2009) Epidemiology, diagnosis, treatment, and control of trichinellosis. Clin Microbiol Rev 22:127–145
Kapel CMO, Gamble HR (2000) Infectivity, persistence, and antibody response to domestic and sylvatic Trichinella spp. in experimentally infected pigs. Int J Parasitol 30:215–221
Kapel CMO, Webster P, Lind P, Pozio E, Henriksen SA, Murrell KD, Nansen P (1998) Trichinella spiralis, Trichinella britovi, and Trichinella nativa: infectivity, larval distribution in muscle, and antibody response after experimental infection of pigs. Parasitol Res 84:264–271
Krivokapich SJ, Gonzalez Prous CL, Gatti GM, Confalonieri V, Molina V, Matarasso H, Guarnera E (2008) Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina. Vet Parasitol 156:234–240
Krivokapich SJ, Pozio E, Gatti GM, Gonzalez Prous CL, Ribicich M, Marucci G, La Rosa G, Confalonieri V (2012) Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America. Int J Parasitol 42:903–910
Leclair D, Forbes LB, Suppa S, Proulx JF, Gajadhar AA (2004) A preliminary investigation on the infectivity of Trichinella larvae in traditional preparations of walrus meat. Parasitol Res 93:507–509
Malakauskas A, Kapel CMO (2003) Tolerance to low temperatures of domestic and sylvatic Trichinella spp. in rat muscle tissue. J Parasitol 89:744–748
McFadden SL, Woo JM, Michalak N, Dv D (2005) Dietary vitamin C supplementation reduces noise-induced hearing loss in guinea pigs. Hear Res 202(1):200–208
Nasinyama GW, Gordon JC, Bech-Nielsen S, Barriga OO (1991) IgG response in guinea pigs to Trichinella spiralis infection. Vet Parasitol 39(3–4):301–311
Oivanen L, Mikkonen T, Haltia L, Karhula H, Saloniemi H, Sukura A (2002) Persistence of Trichinella spiralis in rat carcasses experimentally mixed in different feed. Acta Vet Scand 43:203–210
Owen IL, Reid SA (2007) Survival of Trichinella papuae muscle larvae in a pig carcass maintained under simulated natural conditions in Papua New Guinea. J Helminthol 81:429–432
Pasqualetti M, Fariña F, Falzoni E, Cardillo N, Aronowicz T, Krivokapich S, Rosa A, Ribicich M (2014) Susceptibility of chickens (Gallus gallus domesticus) to Trichinella patagoniensis. Vet Parasitol 205(1–2):397–400
Pozio E (2002) Factors affecting the flow among domestic, synanthropic and sylvatic cycles of Trichinella. Vet Parasitol 93:241–262
Pozio E, La Rosa G, Rossi P, Murrell KD (1992) Biological characterization of Trichinella isolates from various host species and geographical regions. J Parasitol 78:647–653
Ribicich M, Gamble HR, Rosa A, Bolpe J, Franco A (2005) Trichinellosis in Argentina: an historical review. Vet Parasitol 132:137–142
Ribicich M, Krivokapich S, Pasqualetti M, Gonzalez Prous CL, Gatti GM, Falzoni E, Aronowicz T, Arbusti P, Fariña F, Rosa A (2013) Experimental infection with Trichinella T12 in domestic cats. Vet Parasitol 194:168–170
Riva E, Steffan P, Guzmán M, Fiel C (2012) Persistence of Trichinella spiralis muscle larvae in natural decaying mice. Parasitol Res 111:249–255
Segliņa Z, Bakasejevs E, Deksne G, Spuņģis V, Kurjušina M (2015) New finding of Trichinella britovi in a European beaver (Castor fiber) in Latvia. Parasitol Res 114:3171–3173
von Köller J, Kapel CMO, Enemark HL, Hindsbo O (2001) Infectivity of Trichinella spp. recovered from decaying mouse and fox muscle tissue. Parasite 8:209–212
Webster P, Kapel CMO (2005) Studies on vertical transmission of Trichinella spp. in experimentally infected ferrets (Mustela putorius furo), foxes (Vulpes vulpes), pigs, guinea pigs and mice. Vet Parasitol 130:255–262
This study was supported by grants from MINCyT PICT 2013 – 0965 and UBACyT 20020130100336BA. The research of M.K. and J.I. was financially supported by the The Ministry of Education, Youth and Sports of the Czech Republic (7AMB15AR020 and LH12096), Masaryk University, Brno (MUNI/A/1325/2015) and the Charles University in Prague (PRVOUK P41, UNCE 204017 and SVV 260202/2015).
Compliance with ethical standards
The present research was performed in compliance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and was approved by the Committee for the Use and Care of Laboratory Animals (CICUAL) of the Facultad de Ciencias Veterinarias, University of Buenos Aires, under permit number 2014/01.
About this article
Cite this article
Fariña, F., Pasqualetti, M., Ilgová, J. et al. Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus). Parasitol Res 116, 371–375 (2017). https://doi.org/10.1007/s00436-016-5299-4