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Mitochondrial pathways are involved in Eimeria tenella-induced apoptosis of chick embryo cecal epithelial cells

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An Erratum to this article was published on 27 January 2017

Abstract

Accumulating evidence suggests that Eimeria tenella severely damages the intestinal mucosa in infected poultry, resulting in deadly haemorrhagic typhlocolitis and major economic losses. Damage to host tissue is believed to arise mainly from apoptosis, which is, in general, intimately related to mitochondrial function. However, it is unclear whether mitochondria-dependent apoptotic pathways are specifically involved in parasite-induced apoptosis of chick embryo cecal epithelial cells. Because the mitochondrial permeability transition pore (MPTP) and caspase-9 are important elements in these pathways, we studied the effects of their respective inhibitors (i.e., cyclosporine A [CsA] and Z-LEHD-FMK, respectively) in primary cultures of chicken embryonic cecum epithelial cells using histopathological techniques, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assays, flow cytometry (FCM) and ELISA. Results indicated that the inhibitors significantly decreased (p < 0.01) DNA injury, apoptosis and caspase-9 and caspase-3 activity of chick embryo cecal epithelial cells at 24, 48, 72, 96 and 120 h after E. tenella infection. Thus, our data supported that mitochondria-dependent apoptotic pathways were involved in apoptosis of parasitised chick embryo cecal epithelial cells.

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Acknowledgments

This study was funded by a grant from the National Natural Science Foundation of China (Grant No. 31272536).

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Authors and Affiliations

  1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People’s Republic of China

    Shan Li, Ming-xue Zheng, Huan-cheng Xu, Xiao-zhen Cui, Yan Zhang, Li Zhang, Sha-sha Yang, Zhi-yong Xu, Rui Bai & Xu-guang Sun

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  1. Shan Li
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  2. Ming-xue Zheng
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  3. Huan-cheng Xu
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  4. Xiao-zhen Cui
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  5. Yan Zhang
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  6. Li Zhang
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Correspondence to Ming-xue Zheng.

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All work involving animals was conducted in accordance with the national legislations concerning the protection of animal welfare and following the strict guidelines and approval of the Institution Animal Care and Use Committee of Shanxi Agricultural University, China.

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An erratum to this article is available at http://dx.doi.org/10.1007/s00436-017-5390-5.

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Li, S., Zheng, Mx., Xu, Hc. et al. Mitochondrial pathways are involved in Eimeria tenella-induced apoptosis of chick embryo cecal epithelial cells. Parasitol Res 116, 225–235 (2017). https://doi.org/10.1007/s00436-016-5283-z

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  • DOI: https://doi.org/10.1007/s00436-016-5283-z

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