Parasitology Research

, Volume 116, Issue 1, pp 155–165

Coexistence of free-living amoebae and bacteria in selected South African hospital water distribution systems

  • P. Muchesa
  • M. Leifels
  • L. Jurzik
  • K. B. Hoorzook
  • T. G. Barnard
  • C. Bartie
Original Paper

DOI: 10.1007/s00436-016-5271-3

Cite this article as:
Muchesa, P., Leifels, M., Jurzik, L. et al. Parasitol Res (2017) 116: 155. doi:10.1007/s00436-016-5271-3
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Abstract

Pathogenic free-living amoebae (FLA), such as Naegleria fowleri, Balamuthia mandrillaris and Acanthamoeba species isolated from aquatic environments have been implicated in central nervous system, eye and skin human infections. They also allow the survival, growth and transmission of bacteria such as Legionella, Mycobacteria and Vibrio species in water systems. The purpose of this study was to investigate the co-occurrence of potentially pathogenic FLA and their associated bacteria in hospital water networks in Johannesburg, South Africa. A total of 178 water (n = 95) and swab (n = 83) samples were collected from two hospital water distribution systems. FLA were isolated using the amoebal enrichment technique and identified using PCR and 18S rDNA sequencing. Amoebae potentially containing intra-amoebal bacteria were lysed and cultured on blood agar plates. Bacterial isolates were characterized using the VITEK®2 compact System. Free-living amoebae were isolated from 77 (43.3 %) of the samples. Using microscopy, PCR and 18S rRNA sequencing, Acanthamoeba spp. (T3 and T20 genotypes), Vermamoeba vermiformis and Naegleria gruberi specie were identified. The Acanthamoeba T3 and T20 genotypes have been implicated in eye and central nervous system infections. The most commonly detected bacterial species were Serratia marcescens, Stenotrophomonas maltophilia, Delftia acidovorans, Sphingomonas paucimobilis and Comamonas testosteroni. These nosocomial pathogenic bacteria are associated with systematic blood, respiratory tract, the urinary tract, surgical wounds and soft tissues infections. The detection of FLA and their associated opportunistic bacteria in the hospital water systems point out to a potential health risk to immune-compromised individuals.

Keywords

Amoebal enrichment Acanthamoeba spp. Vermamoeba vermiformis Serretia marcescens 

Supplementary material

436_2016_5271_MOESM1_ESM.pdf (23 kb)
ESM 1(PDF 22 kb)

Funding information

Funder NameGrant NumberFunding Note
Water Research Commission
  • K5 /2138

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • P. Muchesa
    • 1
  • M. Leifels
    • 2
  • L. Jurzik
    • 2
  • K. B. Hoorzook
    • 1
  • T. G. Barnard
    • 1
  • C. Bartie
    • 1
  1. 1.Water and Health Research Centre, Faculty of Health SciencesUniversity of JohannesburgDoornfonteinSouth Africa
  2. 2.Department of Hygiene, Social and Environmental MedicineRuhr-University BochumBochumGermany

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