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Molecular survey of Ehrlichia canis and Coxiella burnetii infections in wild mammals of southern Italy

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Abstract

Ehrlichiosis and Q fever caused by the intracellular bacteria Ehrlichia canis and Coxiella burnetii, respectively, are tick-borne diseases with zoonotic potential and widespread geographical distribution. This study investigated the prevalence of both infections in wild mammals in southern Italy. Tissue samples obtained from the red fox (Vulpes vulpes), European badger (Meles meles), gray wolf (Canis lupus), beech marten (Martes foina), and crested porcupine (Hystrix cristata) were processed for molecular detection of both pathogens. E. canis was detected in 55 out of 105 (52 %) red foxes and three out of six gray wolves. Four sequence types were identified, three of which were found in the spleen and liver samples of red foxes and wolves, and one in the kidney of a red fox. None of the examined mammals was positive to C. burnetii type. This represents the first report of E. canis in free-ranging wolves worldwide, as well as the first evidence of this pathogen in red foxes in the peninsular Italy. Our results suggest that E. canis infection is common in free-ranging canids in southern Italy and that a sylvatic life cycle of this pathogen may occur.

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References

  • Amyx HL, Huxsoll DL (1973) Red and gray foxes—potential reservoir hosts for Ehrlichia canis. J Wildl Dis 9:47–50. doi:10.7589/0090-3558-9.1.47

    Article  CAS  PubMed  Google Scholar 

  • Capuano F, Parisi A, Cafiero MA, Pitaro L, Fenizia D (2004) Coxiella burnetii: what is the reality? Parassitologia 46(1-2):131–4

    CAS  PubMed  Google Scholar 

  • Cardoso L, Gilad M, Cortes HCE, Nachum-Biala Y, Lopes AP, Vila-Viçosa MJ, Simões M, Rodrigues PA, Baneth G (2015) First report of Anaplasma platys infection in red foxes (Vulpes vulpes) and molecular detection of Ehrlichia canis and Leishmania infantum in foxes from Portugal. Parasit Vectors 7:113. doi:10.1186/s13071-015-0756-y

    Article  Google Scholar 

  • Curioni V, Cerquetella S, Scuppa P, Pasqualini L, Beninati T, Favia G (2004) Lyme disease and babesiosis: preliminary findings on the transmission risk in highly frequented areas of the Monti Sibillini National Park (Central Italy). Vector Borne Zoonotic Dis 4(3):214–20

    Article  CAS  PubMed  Google Scholar 

  • Dantas-Torres F, Otranto D (2013) Species diversity and abundance of ticks in three habitats in southern Italy. Ticks Tick Borne Dis 4(3):251–5. doi:10.1016/j.ttbdis.2012.11.004

    Article  PubMed  Google Scholar 

  • Ebani VV, Verin R, Fratini F, Poli A, Cerri D (2011) Molecular survey of Anaplasma phagocytophilum and Ehrlichia canis in red foxes (Vulpes vulpes) from central Italy. J Wildl Dis 47:699–703

    Article  PubMed  Google Scholar 

  • Ewing SA, Bucker RG, Springer BC (1964) The coyote, a potential host for Babesia canis and Ehrlichia sp. J Parasitol 50:704

  • Groves MG, Dennis GL, Amyx HL, Huxsoll DL (1975) Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus). Am J Vet Res 36(7):937–40

    CAS  PubMed  Google Scholar 

  • Harris S, Thompson GB (1978) Populations of the ticks Ixodes (Pholeoixodes) hexagonus and Ixodes (Pholeoixodes) canisuga infesting suburban foxes, Vulpes vulpes. J Zool 186:83–93

    Article  Google Scholar 

  • Harrus S, Waner T, Aizenberg I, Foley JE (1998) Amplification of Ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. J Clin Microbiol 36(1):73–6

    CAS  PubMed  PubMed Central  Google Scholar 

  • Harvey JW, Simpson CF, Gaskin JM, Sameck JH (1979) Ehrlichiosis in wolves, dogs, and wolf-dog crosses. J Am Vet Med Assoc 175:901–905

    CAS  PubMed  Google Scholar 

  • Hodžić A, Alić A, Fuehrer HP, Harl J, Wille-Piazzai W, Duscher GG (2015) A molecular survey of vector-borne pathogens in red foxes (Vulpes vulpes) from Bosnia and Herzegovina. Parasit Vectors 8:88. doi:10.1186/s13071-015-0692-x

    Article  PubMed  PubMed Central  Google Scholar 

  • Hornok S, de la Fuente J, Horváth G, de Mera IGF, Wijnveld M, Tánczos B, Farkas R, Jongejan F (2013) Molecular evidence of Ehrlichia canis and Rickettsia massiliae in ixodis ticks of carnivore from south Hungary. Acta Vet Hung 61(1):42–50. doi:10.1556/AVet.2012.050

  • Kazar J (2005) Coxiella burnetii infection. Ann N Y Acad Sci 1063:105–14

    Article  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Komiya T, Sadamasu K, Kang MI, Tsuboshima S, Fukushi H, Hirai K (2003) Seroprevalence of Coxiella burnetii infections among cats in different living environments. J Vet Med Sci 2003(9):1047–8

    Article  Google Scholar 

  • Landon DB, Waite CA, Peterson RO, Mech LD (1998) Evaluation of age determination techniques for gray wolves. J Wildl Manage 62(2):674–682

    Article  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  • Lorusso V, Lia RP, Dantas-Torres F, Mallia E, Ravagnan S, Capelli G, Otranto D (2011) Ixodid ticks of road-killed wildlife species in southern Italy: new tick-host associations and locality records. Exp Appl Acarol 55(3):293–300

    Article  PubMed  Google Scholar 

  • Meredith AL, Cleaveland SC, Denwood MJ, Brown JK, Shaw DJ (2015) Coxiella burnetii (Q-fever) seroprevalence in prey and predators in the United Kingdom: evaluation of infection in wild rodents, foxes and domestic cats using a modified ELISA. Transbound Emerg Dis 62(6):639–49. doi:10.1111/tbed.12211

    Article  CAS  PubMed  Google Scholar 

  • Millán J, Proboste T, Fernández de Mera IG, Chirife AD, de la Fuente J, Altet L (2016) Molecular detection of vector-borne pathogens in wild and domestic carnivores and their ticks at the human-wildlife interface. Ticks Tick Borne Dis 7(2):284–90. doi:10.1016/j.ttbdis.2015.11.003

    Article  PubMed  Google Scholar 

  • Otranto D, Cantacessi C, Pfeffer M, Dantas-Torres F, Brianti E, Deplazes P, Genchi C, Guberti V, Capelli G (2015) The role of wild canids and felids in spreading parasites to dogs and cats in Europe. Part I: Protozoa and tick-borne agents. Vet Parasitol 213(1-2):12–23. doi:10.1016/j.vetpar.2015.04.022

    Article  PubMed  Google Scholar 

  • Pascucci I, Di Domenico M, Dall’Acqua F, Sozio G, Cammà C (2015) Detection of Lyme disease and Q fever agents in wild rodents in central Italy. Vector Borne Zoonotic Dis 15(7):404–11. doi:10.1089/vbz.2015.1807

    Article  PubMed  PubMed Central  Google Scholar 

  • Perez M, Rikihisa Y, Wen B (1996) Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J Clin Microbiol 34:2133–2139

    CAS  PubMed  PubMed Central  Google Scholar 

  • Perez M, Bodor M, Zhang C, Xiong Q, Rikihisa Y (2006) Human infection with Ehrlichia canis accompanied by clinical signs in Venezuela. Ann N Y Acad Sci 1078:110–117

    Article  CAS  PubMed  Google Scholar 

  • Pusterla N, Deplazes P, Braun U, Lutz H (1999) Serological evidence of infection with Ehrlichia spp. in red foxes (Vulpes vulpes) in Switzerland. J Clin Microbiol 37:1168–1169

    CAS  PubMed  PubMed Central  Google Scholar 

  • René-Martellet M, Lebert I, Chêne J, Massot R, Leon M, Leal A, Badavelli S, Chalvet-Monfray K, Ducrot C, Abrial D, Chabanne L, Halos L (2015) Diagnosis and incidence risk of clinical canine monocytic ehrlichiosis under field conditions in Southern Europe. Parasit Vectors 8:3. doi:10.1186/s13071-014-0613-4

    Article  PubMed  PubMed Central  Google Scholar 

  • Roulichová J, Anděra M (2007) Simple method of age determination in red fox, Vulpes vulpes. Folia Zool 56(4):440–444

    Google Scholar 

  • Sainz Á, Roura X, Miró G, Estrada-Peña A, Kohn B, Harrus S, Solano-Gallego L (2015) Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Parasit Vectors 8:75. doi:10.1186/s13071-015-0649-0

    Article  PubMed  PubMed Central  Google Scholar 

  • Schneeberger PM, Hermans MH, van Hannen EJ, Schellekens JJ, Leenders AC, Wever PC (2010) Real-time PCR on serum samples is indispensable for early diagnosis of acute Q fever. Clin Vaccine Immunol 17:286–290

    Article  CAS  PubMed  Google Scholar 

  • Solano-Gallego L, Trotta M, Razia L, Furlanello T, Caldin M (2006) Molecular survey of Ehrlichia canis and Anaplasma phagocytophilum from blood of dogs in Italy. Ann N Y Acad Sci 1078:515–518

    Article  CAS  PubMed  Google Scholar 

  • Stich RW, Schaefer JJ, Bremer WG, Needham GR, Jittapalapong S (2008) Host surveys, ixodid tick biology and transmission scenarios as related to the tick-borne pathogen, Ehrlichia canis. Vet Parasitol 158:256–273

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Bio Evol 30:2725–2729. doi:10.1093/molbev/mst197

    Article  CAS  Google Scholar 

  • Torina A, Blanda V, Antoci F, Scimeca S, D’Agostino R, Scariano E, Piazza A, Galluzzo P, Giudice E, Caracappa S (2013) A molecular survey of Anaplasma spp., Rickettsia spp., Ehrlichia canis and Babesia microti in foxes and fleas from Sicily. Transbound Emerg Dis 60(2):125–30. doi:10.1111/tbed.12137

    Article  PubMed  Google Scholar 

  • Van Heerden J, Mills MG, Van Vuuren MJ, Kelly PJ, Dreyer MJ (1995) An investigation into the health status and diseases of wild dogs (Lycaon pictus) in the Kruger National Park. J S Afr Vet Assoc 66:18–27

    PubMed  Google Scholar 

  • Waner T, Nachum-Biala Y, Harrus S (2014) Evaluation of a commercial in-clinic point-of-care polymerase chain reaction test for Ehrlichia canis DNA in artificially infected dogs. Vet J 202(3):618–21. doi:10.1016/j.tvjl.2014.10.004

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055, Portici, Naples, Italy

    Mario Santoro, Nicola D’Alessio, Francesca Di Prisco, Maria Gabriella Lucibelli, Giorgia Borriello, Anna Cerrone & Giorgio Galiero

  2. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy

    Vincenzo Veneziano

  3. Department of Immunology, Aggeu Magalhães Research Center, 50740465, Recife, Pernambuco, Brazil

    Filipe Dantas-Torres

  4. Department of Veterinary Medicine, University of Bari, 70010, Valenzano, Bari, Italy

    Filipe Dantas-Torres, Maria Stefania Latrofa & Domenico Otranto

Authors
  1. Mario Santoro
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  2. Vincenzo Veneziano
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  3. Nicola D’Alessio
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  4. Francesca Di Prisco
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  5. Maria Gabriella Lucibelli
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  7. Anna Cerrone
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  8. Filipe Dantas-Torres
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  9. Maria Stefania Latrofa
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  10. Domenico Otranto
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  11. Giorgio Galiero
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Correspondence to Mario Santoro.

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Santoro, M., Veneziano, V., D’Alessio, N. et al. Molecular survey of Ehrlichia canis and Coxiella burnetii infections in wild mammals of southern Italy. Parasitol Res 115, 4427–4431 (2016). https://doi.org/10.1007/s00436-016-5213-0

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  • DOI: https://doi.org/10.1007/s00436-016-5213-0

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