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Morphology and small subunit rDNA-based phylogeny of Ceratomyxa amazonensis n. sp. parasite of Symphysodon discus, an ornamental freshwater fish from Amazon

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Abstract

The specious genus Ceratomyxa Thélodan, 1892, infect mainly gallbladder of marine fishes, with only five species reported infecting species from freshwater environment. This study performed morphological and phylogenetic analyses involving a new Ceratomyxa species (Ceratomyxa amazonensis n. sp.) found in gallbladder of Symphysodon discus Heckel, 1840 (Perciformes: Cichlidae), an important ornamental fish endemic to Amazon basin. Mature spores were strongly arcuate shaped and measured 7.0 ± 0.3 (6.2–7.6) μm in length, 15.8 ± 0.4 (15.0–16.7) μm in thickness, and polar capsules 3.22 ± 0.34 (2.4–3.6) μm in length and 2.63 ± 0.17 (2.4–2.9) μm in width. This was the first small subunit ribosomal DNA (SS rDNA) sequencing performed to Ceratomyxa species parasite of freshwater fish, and the phylogenetic analysis showed C. amazonensis n. sp. clustering in the early diverging subclade of the ceratomyxids, together with species of parasites of amphidromous/estuaries fishes, suggesting some role of the transition of the fishes between marine/freshwater environments in the evolutionary history of these parasites.

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Acknowledgments

The study was supported by São Paulo Research Foundation-FAPESP (Proc. No. 2013/21374-6-Adriano E. A.). P.D. Mathews was supported by a doctoral scholarship from FAPESP (Proc. No. 2013/14656-5). J. Naldoni was supported by a Post Doctoral scholarship from FAPESP (Proc. No. 2014/22700-7). E.A. Adriano received a research productivity grant from the National Council of Scientific and Technological Development (CNPq) (Proc. No. 305630/2013-0).

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Authors and Affiliations

  1. Department of Animal Biology, Institute of Biology, Campinas State University, Caixa Postal 6109, CEP 13083-970, Campinas, SP, Brazil

    Patrick D. Mathews & Edson A. Adriano

  2. Department of Biological Sciences, Federal University of São Paulo, Rua Professor Arthur Riedel, 275, Jardim Eldorado, 09972-270, Diadema, SP, Brazil

    Juliana Naldoni & Edson A. Adriano

  3. Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, São Paulo University, Avenida Duque de Caxias Norte, 225, 13635-900, Pirassununga, SP, Brazil

    Antonio A. Maia

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  1. Patrick D. Mathews
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  2. Juliana Naldoni
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  4. Edson A. Adriano
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Correspondence to Edson A. Adriano.

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Mathews, P.D., Naldoni, J., Maia, A.A. et al. Morphology and small subunit rDNA-based phylogeny of Ceratomyxa amazonensis n. sp. parasite of Symphysodon discus, an ornamental freshwater fish from Amazon. Parasitol Res 115, 4021–4025 (2016). https://doi.org/10.1007/s00436-016-5173-4

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