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DEET (N,N-diethyl-meta-toluamide)/PMD (para-menthane-3,8-diol) repellent-treated mesh increases Culicoides catches in light traps

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Abstract

Biting midges (Culicoides spp.) are vectors of bluetongue and Schmallenberg viruses. Treatment of mesh barriers is a common method for preventing insect-vectored diseases and has been proposed as a means of limiting Culicoides ingression into buildings or livestock transporters. Assessments using animals are costly, logistically difficult and subject to ethical approval. Therefore, initial screening of test repellents/insecticides was made by applying treatments to mesh (2 mm) cages surrounding Onderstepoort light traps. Five commercial treatments were applied to cages as per manufacturers’ application rates: control (water), bendiocarb, DEET/p-menthane-3,8-diol (PMD) repellent, Flygo (a terpenoid based repellent) and lambda-cyhalothrin. The experimental design was a 5 × 5 Latin square, replicated in time and repeated twice. Incongruously, the traps surrounded by DEET/PMD repellent-treated mesh caught three to four times more Obsoletus group Culicoides (the commonest midge group) than the other treatments. A proposed hypothesis is that Obsoletus group Culicoides are showing a dose response to DEET/PMD, being attracted at low concentrations and repelled at higher concentrations but that the strong light attraction from the Onderstepoort trap was sufficient to overcome close-range repellence. This study does not imply that DEET/PMD is an ineffective repellent for Culicoides midges in the presence of an animal but rather that caution should be applied to the interpretation of light trap bioassays.

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Acknowledgments

We would like to extend our appreciation to Mr Ian Dodds and the staff of AFBI Loughgall for facilitating this experiment. This work was funded by the Department of Agriculture and Rural Development, Northern Ireland, under the Evidence and Innovation programme.

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  1. Agri-Food and Biosciences Institute, Newforge Lane, Belfast, BT9 5PX, Northern Ireland

    A. K. Murchie, S. Clawson, I. Rea, I. W. N. Forsythe, A. W. Gordon & S. Jess

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  1. A. K. Murchie
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  2. S. Clawson
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  3. I. Rea
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  4. I. W. N. Forsythe
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  5. A. W. Gordon
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Correspondence to A. K. Murchie.

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All experiments were conducted on a research farm by staff trained in pesticide handling and application.

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Murchie, A.K., Clawson, S., Rea, I. et al. DEET (N,N-diethyl-meta-toluamide)/PMD (para-menthane-3,8-diol) repellent-treated mesh increases Culicoides catches in light traps. Parasitol Res 115, 3543–3549 (2016). https://doi.org/10.1007/s00436-016-5119-x

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