Abstract
Order Cyclophyllidea (of cestode platyhelminths) has a rich diversity of parasites and includes many families and species that are known to cause serious medical condition in humans and domestic and wild animals. Despite various attempts to resolve phylogenetic relationships at the inter-family level, uncertainty remains. In order to add resolution to the existing phylogeny of the order, we generated partial mtCO1 sequences for some commonly occurring cyclophyllidean cestodes and combined them with available sequences from GenBank. Phylogeny was inferred taking a total 83 representative species spanning 8 families using Bayesian analysis. The phylogenetic tree revealed Dilepididae as the most basal taxon and showed early divergence in the phylogenetic tree. Paruterinidae, Taeniidae and Anoplocephalidae showed non-monophyletic assemblage; our result suggests that the family Paruterinidae may represent a polyphyletic group. The diverse family Taeniidae appeared in two separate clades; while one of them included all the members of the genus Echinococcus and also Versteria, the representatives of the genera Taenia and Hydatigera clubbed in the other clade. A close affinity of Dipylidiidae with Taenia and Hydatigera was seen, whereas existence of a close relationship between Mesocestoididae and Echinococcus (of Taeniidae) is also demonstrated. The crown group comprised the families Anoplocephalidae, Davaineidae, Hymenolepididae and Mesocestoididae, and also all species of the genus Echinococcus and Versteria mustelae; monophyly of these families (excepting Anolplocephalidae) and the genus Echinococcus as well as its sister-taxon relation with V. mustelae is also confirmed. Furthermore, non-monophyly of Anoplocephalidae is suggested to be correlated with divergence in the host selection.
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Acknowledgments
The study was supported by The Indian Council of Medical Research (ICMR)-sponsored project “Molecular characterization of food-borne trematodes and cestodes prevailing in Northeast India,” sanctioned to V.T. and B.R. et al., Departmental Special Assistance (University Grants Commission (UGC)-Special Assistance Programme) in Zoology at North-Eastern Hill University (NEHU), Shillong. The use of facilities at the Bioinformatics Centre, NEHU is thankfully acknowledged.
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Sharma, S., Lyngdoh, D., Roy, B. et al. Molecular phylogeny of Cyclophyllidea (Cestoda: Eucestoda): an in-silico analysis based on mtCOI gene. Parasitol Res 115, 3329–3335 (2016). https://doi.org/10.1007/s00436-016-5092-4
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DOI: https://doi.org/10.1007/s00436-016-5092-4