Parasitology Research

, Volume 115, Issue 8, pp 3203–3208 | Cite as

Multiple infection of amber Succinea putris snails with sporocysts of Leucochloridium spp. (Trematoda)

  • G. L. Ataev
  • A. A. Zhukova
  • А. S. Tokmakova
  • Е. E. Prokhorova
Original Paper


Amber Succinea putris snails were collected in the Leningrad Region (Russia). Some of them were infected with trematodes Leucochloridium paradoxum, Leucochloridium perturbatum and Leucochloridium vogtianum. One snail had triple infection with all these species. Genotyping of sporocysts by ITS1–5.8S–ITS2 nucleotide sequences of ribosomal DNA (rDNA) and phylogenetic analysis were performed. The results confirmed the species identification of sporocysts of Leucochloridium based on the shape and colour of mature broodsacs. Sporocyst broodsacs could leave the host snail on their own, remaining viable in the environment for up to an hour. This ability of sporocysts may prevent the excessive infection of the molluscan host.


Leucochloridium trematodes Broodsacs of sporocysts Multiple infection ITS1–5.8S–ITS2 rDNA genotyping 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • G. L. Ataev
    • 1
  • A. A. Zhukova
    • 1
  • А. S. Tokmakova
    • 1
  • Е. E. Prokhorova
    • 1
  1. 1.Laboratory of Experimental Zoology, Department of Zoology, Faculty of BiologyHerzen State Pedagogical University of RussiaSaint PetersburgRussian Federation

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