Parasitology Research

, Volume 115, Issue 5, pp 1799–1805 | Cite as

Ultrastructure and phylogeny of Kudoa barracudai sp. nov. infecting Sphyraena putnamae in the Red Sea

  • Abdel-Azeem S. Abdel-Baki
  • Saleh Al-Quraishy
  • Suliman Y. Al Omar
  • Lamjed Mansour
Original Paper
First Online:
Received:
Accepted:

Abstract

Kudoa barracudai n. sp. has been found infecting the muscles of Sphyraena putnamae from Red Sea, Saudi Arabia. The prevalence of the infection was 10 % (5/50). Its ovoidal plasmodia encapsulated with fibrous connective tissue and measuring 700 × 510 μm. Local myolysis was observed around the plasmodia without inflammatory reaction. Mature spores were quadrate in the apical view with rounded edges and subspherical to ovoid in the side view and measured 5 μm (4.5–5.5) in width and 5.5 μm (5–6) in thickness. Polar capsules were pyriform, equal in size, and measured 2.5 μm (2–3) in length and 1.5 μm (1–2) in width with two filament coils. Ultrastructural analysis showed a demarcated border between the parasite cellular mass and host cellular components that represented the cyst wall, and cysts were filled with mostly mature spores. Phylogenetic analysis of the small subunit ribosomal RNA (SSU rRNA) using the maximum likelihood and Bayesian inference approaches showed the new species clustered in a robust clade with Kudoa amamiensis, Kudoa kenti, and Kudoa quraishii. The SSU rRNA gene of K. barracudai was most similar to K. amamiensis (98.7 %), followed by K. kenti (97.4 %) and K. quraishii (96.6 %). This combination of morphological data and molecular analysis served to identify this parasite as a new species of Kudoa, which we have named K. barracudai n. sp.

Keywords

Muscle Myxozoa New species Parasite SSU rRNA gene 

Notes

Acknowledgments

We extend our appreciation to the Dean of Scientific Research, King Saud University, for funding the work through the research group project number RG −004.

References

  1. Al Quraishy S, Koura E, Abdel-Baki AS, Bashtar AR, El Deed N, Al Rasheid K, Abdel Ghaffar F (2008) Light and electron microscopic studies on Kudoa pagrusi sp. n. (Myxosporea: Multivalvulida) infecting the heart of sea bream Pagrus pagrus (L.) from the Red Sea. Parasitol Res 102:205–209CrossRefPubMedGoogle Scholar
  2. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefPubMedPubMedCentralGoogle Scholar
  3. Burger MA, Adlard RD (2010) Four new species of Kudoa Meglitsch, 1947 (Myxosporea: Multivalvulida) from Australia with recommendations for species descriptions in the Kudoidae. Parasitology 137:793–814CrossRefPubMedGoogle Scholar
  4. Doiuchi R, Nakabo T (2005) The Sphyraena obtusata group (Perciformes: Sphyraenidae) with a description of a new species from southern Japan. Ichthyol Res 52:132–151CrossRefGoogle Scholar
  5. Egusa S, Nakajima K (1980) Kudoa amamiensis n. sp. (Myxosporea: Multivalvulida) found in cultured yellowtails and wild damselfishes from Amami-Ohshima and Okinawa, Japan. Bull Jpn Soc Sci Fish 46:1193–1198CrossRefGoogle Scholar
  6. Egusa S, Shiomitsu T (1983) Two new species of the genus Kudoa (Myxosporea: Multivalvulida) from marine cultured fishes in Japan. Fish Pathol 18:163–171CrossRefGoogle Scholar
  7. Eiras JC, Saraiva A, Cruz C (2014) Synopsis of the species of Kudoa Meglitsch, 1947 (Myxozoa: Myxosporea: Multivalvulida). Syst Parasitol 87:153–180CrossRefPubMedGoogle Scholar
  8. Hervio DML, Kent ML, Khattra J, Sakanari J, Yokoyama H, Devlin RH (1997) Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA. Can J Zool 75:2112–2119CrossRefGoogle Scholar
  9. Kristmundsson A, Freeman MA (2014) Negative effects of Kudoa islandica n. sp. (Myxosporea: Kudoidae) on aquaculture and wild fisheries in Iceland. Int J Parasitol Parasites Wildl 3:135–146CrossRefPubMedPubMedCentralGoogle Scholar
  10. Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156CrossRefGoogle Scholar
  11. Mansour L, Thabet A, Chourabi K, Harrath AH, Gtari M, Al Omar SY, Ben Hassine OK (2013) Kudoa azevedoi n. sp. (Myxozoa, Multivalvulida) from the oocytes of the Atlantic horse mackerel Trachurus trachurus (Perciformes, Carangidae) in Tunisian coasts. Parasitol Res 112:1737–1747CrossRefPubMedGoogle Scholar
  12. Mansour L, Harrath AH, Abd-Elkader OH, Alwasel S, Abdel-Baki AS, Al Omar SY (2014) Structural and molecular characterization of Kudoa quraishii n. sp. from the trunk muscle of the Indian mackerel Rastrelliger kanagurta (Perciforme, Scombridae) in Saudi Arabia coasts. Parasitol Res 113:1361–1370CrossRefPubMedGoogle Scholar
  13. Mansour L, Harrath A, Abdel-Baki AS, Alwasel S, Al-Quraishy S, Al Omar SY (2015) Kudoa saudiensis sp. n. (Myxosporea: Multivalvulida) infecting oocytes of the Indian mackerel Rastrelliger kanagurta (Perciformes: Scombridae). Folia Parasitol 62:1–10CrossRefGoogle Scholar
  14. Miller TL, Adlard RD (2012) Brain infecting kudoids of Australia’s coral reefs, including a description of Kudoa lemniscati n. sp. (Myxosporea: Kudoidae) from Lutjanus lemniscatus (Perciformes: Lutjanidae) off Ningaloo Reef, Western Australia. Parasitol Int 61:333–342CrossRefPubMedGoogle Scholar
  15. Narasimhamurti CC, Kalavati C (1979) Kudoa sphyraeni n.sp. (Myxosporidia: Protozoa) parasitic in the muscles of the gut of the marine fish, Sphyraena jello Cuv. Proc Indian Acad Sci 88:265–268CrossRefGoogle Scholar
  16. Pastore MA (2009) Sphyraena intermedia sp. nov. (Pisces: Sphyraenidae): a potential new species of barracuda identified from the central Mediterranean Sea. J Mar Biol Ass UK 89:1299–1303CrossRefGoogle Scholar
  17. Shirakashi S, Yamane K, Ishitani H, Yanagida T, Yokoyama H (2014) First report of Kudoa species in the somatic muscle of the Japanese parrotfish Calotomus japonicus (Scaridae) and a description of Kudoa igami, n. sp. (Myxozoa: Multivalvulida). Parasitol Res 113:2515–2524CrossRefPubMedGoogle Scholar
  18. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  19. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefPubMedPubMedCentralGoogle Scholar
  20. Whipps CM, Grossel G, Adlard RD, Yokoyama H, Bryant MS, Munday BL, Kent ML (2004) Phylogeny of the multivalvulidae (Myxozoa: Myxosporea) based on comparative ribosomal DNA sequence analysis. J Parasitol 90:618–622CrossRefPubMedGoogle Scholar
  21. Yokoyama H, Itoh N (2005) Two multivalvulid myxozoans causing postmortem myoliquefaction: Kudoa megacapsula n. sp. from red barracuda (Sphyraena pinguis) and Kudoa thyrsites from splendid alfonso (Beryx splendens). J Parasitol 91:1132–1137CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Abdel-Azeem S. Abdel-Baki
    • 1
    • 2
  • Saleh Al-Quraishy
    • 1
  • Suliman Y. Al Omar
    • 1
  • Lamjed Mansour
    • 1
    • 3
  1. 1.Zoology Department, College of ScienceKing Saud University, Saudi ArabiaRiyadhSaudi Arabia
  2. 2.Zoology Department, Faculty of ScienceBeni-Suef UniversityBeni-SuefEgypt
  3. 3.Unité de Recherche de Biologie intégrative et Ecologie évolutive et Fonctionnelle des Milieux Aquatiques, Département de Biologie, Faculté des Sciences de TunisUniversité De Tunis El ManarTunisTunisia

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