Abstract
Opisthorchis viverrini is highly prevalent throughout Southeast Asia. Chronic infection of this parasite leads to cholangiocarcinoma (CCA), a fatal bile duct cancer. The early and accurate detection of this parasite is very important; therefore, new PCR methods targeting cytochrome c oxidase subunit one and NADH dehydrogenase subunit one gene to detect O. viverrini in fecal specimens have been developed. Ninety O. viverrini-positive human fecal samples were used in this study. The PCR sensitivity of both genes was compared with internal transcribe spacer 2 (ITS2)-PCR. The sensitivity of cox1-PCR and nad1-PCR was 66.7 and 50 %, respectively. The sensitivity of cox1-PCR and nad1-PCR achieved 89.1 and 71.7 % in specimens containing O. viverrini eggs of >100 eggs per gram (EPG). Additionally, these primers can be used to provide the information on genetic diversity from mitochondrial genes of O. viverrini.
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Acknowledgement
The authors gratefully acknowledge the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/1076/2552), Thailand Research Fund (TRF), Mahidol University, and the Phramongkutklao Research Fund for supporting this study.
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Buathong, S., Leelayoova, S., Mungthin, M. et al. Development and evaluation of PCR methods based on cytochrome c oxidase subunit one (cox1) and NADH dehydrogenase subunit one gene (nad1) to detect Opisthorchis viverrini in human fecal samples. Parasitol Res 114, 3547–3549 (2015). https://doi.org/10.1007/s00436-015-4640-7
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DOI: https://doi.org/10.1007/s00436-015-4640-7