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Genetic and morphological characterization of Trichuris myocastoris found in Myocastor coypus in the Czech Republic

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Abstract

Trichuris sp. individuals were collected from Myocastor coypus from fancy breeder farms in the Czech Republic. Using morphological and biometrical methods, 30 female and 30 male nematodes were identified as Trichuris myocastoris. This paper presents the first molecular description of this species. The ribosomal DNA (rDNA) region, consisting of internal transcribed spacer (ITS)-1, 5.8 gene and ITS-2, was sequenced. Based on an analysis of 651 bp, T. myocastoris was found to be different from any other Trichuris species for which published sequencing of the ITS region is available. The phylogenetic relationships were estimated using the maximum parsimony methods and Bayesian analyses. T. myocastoris was found to be significantly closely related to Trichuris of rodents than those of ruminants.

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Acknowledgments

This study has been funded by the Ministry of Agriculture of the Czech Republic (Nutria Genetic Resources Programme) and the Grant of National Agency for Agricultural Research of the Czech Republic, v.v.i. CIGA No. 20152021. We would also like to thank Mr. Brian Kavalir for his proofreading services.

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Authors and Affiliations

  1. Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic

    K. Rylková, A. Brožová, I. Jankovská, J. Vadlejch, Z. Čadková, J. Frýdlová, P. Peřinková, I. Langrová, S. Nechybová & Š. Scháňková

  2. Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic

    E. Tůmová & D. Chodová

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  1. K. Rylková
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  2. E. Tůmová
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  3. A. Brožová
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  4. I. Jankovská
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  5. J. Vadlejch
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  6. Z. Čadková
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  7. J. Frýdlová
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  8. P. Peřinková
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  9. I. Langrová
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  10. D. Chodová
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  11. S. Nechybová
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  12. Š. Scháňková
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Correspondence to I. Langrová.

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Rylková, K., Tůmová, E., Brožová, A. et al. Genetic and morphological characterization of Trichuris myocastoris found in Myocastor coypus in the Czech Republic. Parasitol Res 114, 3969–3975 (2015). https://doi.org/10.1007/s00436-015-4623-8

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  • DOI: https://doi.org/10.1007/s00436-015-4623-8

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