Abstract
Psoroptidae mites are a type of small mites with a wide range of hosts. The proliferation of Psoroptidae mites could cause symptoms such as severe itching, atopic dermatitis, and hair loss in infected animals. If severely infected, death can also occur. The morphological classification and identification of Psoroptidae mites is problematic due to the overlapping geographical distribution. In addition, there is no divergence threshold for molecular classification and identification. To solve this problem, gDNA was extracted from individual Psoroptes and Otodectes mites (China) for amplification of rDNA ITS2 and mtDNA COI. After that, the sequences obtained were aligned and analyzed with those retrieved from GenBank. Based on rDNA ITS2 sequences, Psoroptidae was divided into three genera, namely, Psoroptes, Chorioptes, and Otodectes, which was in accordance with morphological classification. The intraspecies, interspecies, and intergenera could be differentiated effectively, with thresholds ≤ 5.20, 6.18–14.86, and ≥15.72 %, respectively. However, based on mtDNA COI sequences, Psoroptidae was divided into four genera with Caparinia added, as Caparinia sp did not cluster with the other three genera. The intra- and interspecies could be differentiated effectively, but interspecies and intergenera could not. The intra- and interspecies identification thresholds were ≤ 2.12 and ≥10.93 %. Further analysis showed that host but not geographical isolation was found in Psoroptes and Chorioptes, whereas Otodectes mites parasitizing dogs and cats were the same species; neither host nor geographical isolation was observed. In conclusion, rDNA ITS2 is better than mtDNA COI for DNA barcoding in Psoroptidae.
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References
Alasaad S, Rossi L, Maione S, Sartore S, Soriguer RC, Pérez JM, Rasero R, Zhu XQ, Soglia D (2008) HotSHOT Plus ThermalSHOCK, a new and efficient technique for preparation of PCR-quality mite genomic DNA. Parasitol Res 103:1455–1457
Andre VB, Pavel BK, Gete H, Alexander PS (2014) Integrated Bayesian species delimitation and morphological diagnostics of chorioptic mange mites (Acariformes: Psoroptidae: Chorioptes). Parasitol Res 113:2603–2627
Blot C, Kodjo A, Reynaud MC, Bourdoiseau G (2003) Efficacy of selamectin administered topically in the treatment of feline otoacariosis. Vet Parasitol 112:241–247
Burgess S, Downing A, Watkins CA, Marr EJ, Nisbet AJ, Kenyon F, McNair C, Huntley JF (2012) Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis. Parasite Vector 5:30
Chitimia L, Lin RQ, Cosoroaba I, Braila P, Song HQ, Zhu XQ (2009) Molecular characterization of hard and soft ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA. Parasitol Res 105:907–911
Essig A, Rinder H, Gothe R, Zahler M (1999) Genetic differentiation of mites of the genus Chorioptes (Acari: Psoroptidae). Exp Appl Acarol 23:309–318
Gu XB, Liu GH, Song HQ, Liu TY, Yang GY, Zhu XQ (2014) The complete mitochondrial genome of the scab mite Psoroptes cuniculi (Arthropoda: Arachnida) provides insights into Acari phylogeny. Parasite Vector 7:340
Hestvik G, Zahler-Rinder M, Gavier-Widén D, Lindberg R, Mattsson R, Morrison D, Bornstein S (2007) A previously unidentified Chorioptes species infesting outer ear canals of moose (Alces alces): characterization of the mite and the pathology of infestation. Acta Vet Scand 49:21–30
Jia XY, Yang GY, Gu XB, Lai SJ (2008) Nucleotide variation in rDNA ITS-2 differentiates Psoroptes isolates from rabbits and buffalo. Acta Entomol Sin 51:545–550
Kato T, Ito Y, Nakao H, Kadosaka T (2011) Tinnitus caused by tiny mites—suspected cases of erratic migration of Otodectes cynotis and a case of Aleuroglyphus ovatus infestation. Med Entomol Zool 62:199–204
Khaing TM, Shim JK, Lee KY (2014) Molecular identification and phylogenetic analysis of economically important acaroid mites (Acari: Astigmata: Acaroidea) in Korea. Entomol Res 44:331–337
Knaus M, Rapti D, Shukullari E, Kusi I, Postoli R, Xhaxhiu D, Silaghi C, Hamel D, Visser M, Winter R, Rehbein S (2014) Characterisation of ecto- and endoparasites in domestic cats from Tirana, Albania. Parasitol Res 113:3361–3371
Lohse J, Rinder H, Gothe R, Zahler M (2002) Validity of species status of the parasitic mite Otodectes cynotis. Med Vet Entomol 16:133–138
Mendes-de-Almeida F, Crissiuma AL, Gershony LC, Willi LMV, Paiva JP, Guerrero J, Labarthe N (2011) Characterization of ectoparasites in an urban cat (Felis catus Linnaeus, 1758) population of Rio de Janeiro, Brazil. Parasitol Res 108:1431–1435
Noge K, Mori N, Tanaka C, Nishida R, Tsuda M, Kuwahara Y (2005) Identification of astigmatid mites using the second internal transcribed spacer (ITS2) region and its application for phylogenetic study. Exp Appl Acarol 35:29–46
Nong X, Li SH, Wang JH, Xie Y, Chen FZ, Liu TF, He R, Gu XB, Peng XR, Yang GY (2014) Acaricidal activity of petroleum ether extracts from Eupatorium adenophorum against the ectoparasitic cattle mite, Chorioptes texanus. Parasitol Res 113:1201–1207
Ochs H, Mathis A, Deplazes P (1999) Single nucleotide variation in rDNA ITS-2 differentiates Psoroptes isolates from sheep and rabbits from the same geographical area. Parasitology 119:419–424
Pegler K, Evans L, Stevens J, Wall R (2005) Morphological and molecular comparison of host-derived populations of parasitic Psoroptes mites. Med Vet Entomol 19:392–403
Sastre N, Ravera I, Ferreira D, Altet L, Sánchez A, Bardagí M, Francino O, Ferrer L (2013) Development of a PCR technique specific for Demodex injai in biological specimens. Parasitol Res 112:3369–3372
Shang XF, Miao XL, Lv HP, Wang DS, Zhang JQ, He H, Yang ZQ, Pan H (2014) Acaricidal activity of usnic acid and sodium usnic acid against Psoroptes cuniculi in vitro. Parasitol Res 113:2387–2390
Wang S, Gu X, Fu Y, Lai S, Wang S, Peng X, Yang G (2012) Molecular taxonomic relationships of Psoroptes and Chorioptes mites from China based on COI and 18S rDNA gene sequences. Vet Parasitol 184:392–397
Xu Y, Yang G, Lai S (2004) The research progress of psoroptic mange. Chin Anim Husb Vet Med 31:42–45
Yang B, Cai J, Cheng X (2011) Identification of astigmatid mites using ITS2 and COI regions. Parasitol Res 108:497–503
Zahler M, Essig A, Gothe R, Rinder H (1998) Genetic evidence suggests that Psoroptes isolates of different phenotypes, hosts and geographic origins are conspecific. Int J Parasitol 28:1713–1719
Zahler M, Hendrix WML, Essig A, Rinder H, Gothe R (2001) Taxonomic reconsideration of the genus Chorioptes Gervais and van Beneden, 1859 (Acari: Psoroptidae). Exp Appl Acarol 25:517–523
Zhao YE, Xu JR, Hu L, Wu LP, Wang ZH (2012) Complete sequence analysis of 18S rDNA based on genomic DNA extraction from individual Demodex mites (Acari: Demodicidae). Exp Parasitol 131:45–51
Zhao YE, Cheng J, Hu L, Ma JX (2014) Molecular identification and phylogenetic study of Demodex caprae. Parasitol Res 113:3601–3608
Zhao YE, Cao ZG, Cheng J, Hu L, Ma JX, Yang YJ, Wang XP, Zeng JH, Wang TP (2015) Population identification of Sarcoptes hominis and Sarcoptes canis in China using DNA sequences. Parasitol Res 114:1001–1010
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This work was supported by the National Natural Science Foundation of China (Nos. 81271856 and 81471972).
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Cheng Juan and Liu ChengCheng contributed equally to this work.
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Juan, C., ChengCheng, L., YaE, Z. et al. Population identification and divergence threshold in Psoroptidae based on ribosomal ITS2 and mitochondrial COI genes. Parasitol Res 114, 3497–3507 (2015). https://doi.org/10.1007/s00436-015-4578-9
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DOI: https://doi.org/10.1007/s00436-015-4578-9