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The life cycle of Ortholinea auratae (Myxozoa: Ortholineidae) involves an actinospore of the triactinomyxon morphotype infecting a marine oligochaete

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Abstract

Actinospores released from the marine oligochaete Limnodriloides agnes inhabiting a Southern Portuguese fish farm are molecularly recognized as developmental stages of the life cycle of Ortholinea auratae, a myxosporean parasite that infects the urinary bladder of Sparus aurata. The molecular analysis of the 18S rRNA gene reveals a similarity of 99.9 to 100 % of the actinospores analyzed to the myxospores of O. auratae. The actinospores belong to the triactinomyxon morphotype and occur in groups of eight within pansporocysts that develop in the intestinal epithelium of the oligochaete host. This is the first record of a myxosporean using an oligochaete as its invertebrate host in the marine environment.

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Acknowledgments

This research was partially supported by the European Regional Development Fund (ERDF) through the COMPETE - Operational Competitiveness Programme and national funds through FCT—Foundation for Science and Technology, under the project “PEst-C/MAR/LA0015/2013” the project DIRDAMyx, reference FCOMP-01-0124-FEDER-020726/FCT-PTDC/MAR/116838/2010, and the Ph.D. fellowship grant SFRH/BD/82237/2011 attributed to L. Rangel and the Ph.D. fellowship grant SFRH/BD/92661/2013 attributed to S. Rocha through the programme POPH/FSE QREN; and the project EUCVOA (NORTE-07-0162-FEDER-000116) (Portugal). This work complies with the current laws of the country where it was performed.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s/n, Edifício FC4, 4169-007, Porto, Portugal

    Luis F. Rangel, Ricardo Castro & Maria J. Santos

  2. Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal

    Luis F. Rangel, Sónia Rocha, Ricardo Castro, Ricardo Severino, Graça Casal, Carlos Azevedo, Francisca Cavaleiro & Maria J. Santos

  3. Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua Jorge Viterbo Ferreira n 228, 4050-313, Porto, Portugal

    Sónia Rocha & Carlos Azevedo

  4. Department of Sciences, High Institute of Health Sciences-North, CESPU, Rua Central da Gandra, 1317, 4585-116, Gandra, Portugal

    Graça Casal

  5. Zoology Department, College of Sciences, King Saud University, 11451, Riyadh, Saudi Arabia

    Carlos Azevedo

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  1. Luis F. Rangel
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  2. Sónia Rocha
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  4. Ricardo Severino
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  7. Francisca Cavaleiro
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  8. Maria J. Santos
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Correspondence to Luis F. Rangel.

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Rangel, L.F., Rocha, S., Castro, R. et al. The life cycle of Ortholinea auratae (Myxozoa: Ortholineidae) involves an actinospore of the triactinomyxon morphotype infecting a marine oligochaete. Parasitol Res 114, 2671–2678 (2015). https://doi.org/10.1007/s00436-015-4472-5

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