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Biochemical and functional characterization of the glutathione S-transferase from Trichinella spiralis

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Abstract

Glutathione-S-transferase (GST) is a family of multifunctional enzymes catalyzing detoxification reactions. Our previous study showed that Trichinella spiralis GST (TsGST) gene is an up-regulated gene in intestinal infective larvae (IIL) compared to muscle larvae (ML) and vaccination of mice with rTsGST displayed a partial protection against challenge infection. The purified rTsGST showed the maximum enzymatic activity at pH 6.5 and 40 °C. The enzymatic K m values for GSH and CDNB were 457 and 123 μM, respectively. An in vitro invasion assay showed that when anti-rTsGST serum of mice infected with T. spiralis and normal mouse serum were added to the medium, and the invasion rate of the infective larvae in an intestinal epithelial cell (IEC) monolayer was 31.0, 11.36, and 78.96 %, respectively (P < 0.05), which indicates that anti-rTsGST antibodies partially inhibited the larval invasion of IEC. ADCC assay showed that anti-rTsGST serum induced significant death of larvae (70 % cytotoxicity) compared to the larvae incubated with pre-immune serum (12 % cytotoxicity, P < 0.001) and was dose dependent.

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References

  • Armstrong RN (1997) Structure, catalytic mechanism, and evolution of the glutathione transferases. Chem Res Toxicol 10(1):2–18

    Article  CAS  PubMed  Google Scholar 

  • Boulanger D et al (1994) Vaccination of goats against the trematode Schistosoma bovis with a recombinant homologous schistosome-derived glutathione S-transferase. Parasite Immunol 16(8):399–406

    Article  CAS  PubMed  Google Scholar 

  • Bruce RG (1970) Structure of the esophagus of the infective juvenile and adult Trichinella spiralis. J Parasitol 56(3):540–549

    Article  CAS  PubMed  Google Scholar 

  • Capron A, Capron M, Dombrowicz D, Riveau G (2001) Vaccine strategies against schistosomiasis: from concepts to clinical trials. Int Arch Allergy Immunol 124(1–3):9–15

    Article  CAS  PubMed  Google Scholar 

  • Corsini E, Liesivuori J, Vergieva T, Van Loveren H, Colosio C (2008) Effects of pesticide exposure on the human immune system. Hum Exp Toxicol 27(9):671–680

    Article  CAS  PubMed  Google Scholar 

  • Cui J et al (2013) Proteomic analysis of surface proteins of Trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry. Parasit Vectors 6:355

    Article  PubMed Central  PubMed  Google Scholar 

  • Despommier DD (1993) Trichinella spiralis and the concept of niche. J Parasitol 79(4):472–482

    Article  CAS  PubMed  Google Scholar 

  • Forsberg L, de Faire U, Morgenstern R (2001) Oxidative stress, human genetic variation, and disease. Arch Biochem Biophys 389(1):84–93

    Article  CAS  PubMed  Google Scholar 

  • Gagliardo LF, McVay CS, Appleton JA (2002) Molting, ecdysis, and reproduction of Trichinella spiralis are supported in vitro by intestinal epithelial cells. Infect Immun 70(4):1853–1859

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Grezel D, Capron M, Grzych JM, Fontaine J, Lecocq JP, Capron A (1993) Protective immunity induced in rat schistosomiasis by a single dose of the Sm28GST recombinant antigen: effector mechanisms involving IgE and IgA antibodies. Eur J Immunol 23(2):454–560

    Article  CAS  PubMed  Google Scholar 

  • Gupta S, Bhandari YP, Reddy MV, Harinath BC, Rathaur S (2005) Setaria cervi: immunoprophylactic potential of glutathione-S-transferase against filarial parasite Brugia malayi. Exp Parasitol 109(4):252–255

    Article  CAS  PubMed  Google Scholar 

  • Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249(22):7130–7139

    CAS  PubMed  Google Scholar 

  • Jiang P, Wang ZQ, Cui J, Zhang X (2012) Comparison of artificial digestion and Baermann’s methods for detection of Trichinella spiralis pre-encapsulated larvae in muscles with low-level infections. Foodborne Pathog Dis 9(1):27–31

    Article  CAS  PubMed  Google Scholar 

  • Laborde E (2010) Glutathione transferases as mediators of signaling pathways involved in cell proliferation and cell death. Cell Death Differ 17(9):1373–1380

    Article  CAS  PubMed  Google Scholar 

  • Li F, Cui J, Wang ZQ, Jiang P (2010) Sensitivity and optimization of artificial digestion in the inspection of meat for Trichinella spiralis. Foodborne Pathog Dis 7(8):879–885

    Article  PubMed  Google Scholar 

  • Liu RD, Wang ZQ, Wang L, Long SR, Ren HJ, Cui J (2013) Analysis of differentially expressed genes of Trichinella spiralis larvae activated by bile and cultured with intestinal epithelial cells using real-time PCR. Parasitol Res 112(12):4113–4120

    Article  PubMed  Google Scholar 

  • ManWarren T, Gagliardo L, Geyer J, McVay C, Pearce-Kelling S, Appleton J (1997) Invasion of intestinal epithelia in vitro by the parasitic nematode Trichinella spiralis. Infect Immun 65(11):4806–4812

    PubMed Central  CAS  PubMed  Google Scholar 

  • McVay CS, Tsung A, Appleton J (1998) Participation of parasite surface glycoproteins in antibody-mediated protection of epithelial cells against Trichinella spiralis. Infect Immun 66(5):1941–1945

    PubMed Central  CAS  PubMed  Google Scholar 

  • Morrison CA et al (1996) Protection of cattle against Fasciola hepatica infection by vaccination with glutathione S-transferase. Vaccine 14(17–18):1603–1612

    Article  CAS  PubMed  Google Scholar 

  • Moskwa B (1999) Trichinella spiralis: in vitro cytotoxicity of peritoneal cells against synchronous newborn larvae of different age. Parasitol Res 85(1):59–63

    Article  CAS  PubMed  Google Scholar 

  • Preyavichyapugdee N, Sahaphong S, Riengrojpitak S, Grams R, Viyanant V, Sobhon P (2008) Fasciola gigantica and Schistosoma mansoni: vaccine potential of recombinant glutathione S-transferase (rFgGST26) against infections in mice. Exp Parasitol 119(2):229–237

    Article  CAS  PubMed  Google Scholar 

  • Ren HJ, Cui J, Wang ZQ, Liu RD (2011) Normal Mouse Intestinal Epithelial Cells as a Model for the in vitro Invasion of Trichinella spiralis Infective Larvae. PLoS One 6(10):e27010

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ren HJ, Cui J, Yang W, Liu RD, Wang ZQ (2013) Identification of differentially expressed genes of Trichinella spiralis larvae after exposure to host intestine milieu. PLoS One 8(6):e67570

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Sheehan D, Meade G, Foley VM, Dowd CA (2001) Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily. Biochem J 360(1):1–16

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Tu CP, Akgul B (2005) Drosophila glutathione S-transferases. Methods Enzymol 401:204–226

    Article  CAS  PubMed  Google Scholar 

  • Veerapathran A, Dakshinamoorthy G, Gnanasekar M, Reddy MVR, Kalyanasundaram R (2009) Evaluation of Wuchereria bancrofti GST as a Vaccine Candidate for Lymphatic Filariasis. Plos Negl Trop Dis 3(6):e457

    Article  PubMed Central  PubMed  Google Scholar 

  • Venturiello SM, Malmassari SL, Costantino SN, Nunez GG (2000) Cytotoxicity-blocking antibodies in human chronic trichinellosis. Parasitol Res 86(9):762–767

    Article  CAS  PubMed  Google Scholar 

  • Wang ZQ, Wang L, Cui J (2012) Proteomic analysis of Trichinella spiralis proteins in intestinal epithelial cells after culture with their larvae by shotgun LC-MS/MS approach. J Proteomics 75(8):2375–2383

    Article  CAS  PubMed  Google Scholar 

  • Wang B, Wang ZQ, Jin J, Ren HJ, Liu LN, Cui J (2013) Cloning, expression and characterization of a Trichinella spiralis serine protease gene encoding a 35.5 kDa protein. Exp Parasitol 134(2):148–154

    Article  CAS  PubMed  Google Scholar 

  • Yang Y et al (2001) Role of glutathione S-transferases in protection against lipid peroxidation. Overexpression of hGSTA2-2 in K562 cells protects against hydrogen peroxide-induced apoptosis and inhibits JNK and caspase 3 activation. J Biol Chem 276(22):19220–19230

    Article  CAS  PubMed  Google Scholar 

  • Yang W et al (2015) Molecular identification and characterization of Trichinella spiralis proteasome subunit beta type-7. Parasit Vectors 8(1):18

    Article  PubMed Central  PubMed  Google Scholar 

  • Zhan B et al (2005) Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum. Infect Immun 73(10):6903–6911

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhan B et al (2010) Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanus. Infect Immun 78(4):1552–1563

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81271860 and 81471981).

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Authors and Affiliations

  1. Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People’s Republic of China

    Jing Cui, Ling Ge Li, Peng Jiang, Ruo Dan Liu, Xuan Yang, Li Na Liu, Pei Liu, Shuai Bing Zhang & Zhong Quan Wang

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  1. Jing Cui
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  2. Ling Ge Li
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  3. Peng Jiang
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  4. Ruo Dan Liu
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  5. Xuan Yang
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  6. Li Na Liu
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  7. Pei Liu
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  8. Shuai Bing Zhang
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  9. Zhong Quan Wang
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Correspondence to Jing Cui or Zhong Quan Wang.

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Cui, J., Li, L.G., Jiang, P. et al. Biochemical and functional characterization of the glutathione S-transferase from Trichinella spiralis . Parasitol Res 114, 2007–2013 (2015). https://doi.org/10.1007/s00436-015-4410-6

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  • DOI: https://doi.org/10.1007/s00436-015-4410-6

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