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Development and optimization of cocktail-ELISA for a unified surveillance of zoonotic schistosomiasis in multiple host species

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Abstract

The zoonotic characteristic of the human parasite Schistosoma japonicum infecting a significant number of wild and domestic animals highlights the need to develop a unified surveillance in multiple host species for a strengthened schistosomiasis control. It has been shown in several studies that water buffaloes and dogs are considered important reservoirs in the transmission of the schistosome parasite to humans. Recombinant antigens like thioredoxin peroxidase-1 (SjTPx-1) and tandem repeat proteins (Sj1TR, Sj7TR) have been shown to be good diagnostic antigens individually in humans, water buffaloes, and dogs in previous studies. Mixing these antigens together in a cocktail-ELISA might not only improve their diagnostic potentials but rather produce a multi-host species detection means for zoonotic schistosomiasis. In this study, we aimed to develop and optimize cocktail-ELISA by testing different combinations of these recombinant antigens in humans, water buffaloes, and dogs. As compared with the diagnostic potential calculated for each of the three recombinant antigens used, their combination has presented improved specificities, positive predictive values, and kappa values. Using samples collected from various endemic areas in the Philippines, results showed that the combination of SjTPx-1/Sj7TR/Sj1TR has the highest sensitivity in humans (84.1 %), water buffaloes, and dogs (80 %) and specificity (100 %) in all host species. This study therefore suggests the use of cocktail-ELISA in improving the zoonotic surveillance in schistosomiasis endemic areas.

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Acknowledgments

We would like to thank the staff of the Department of Parasitology, College of Public Health, University of the Philippines Manila and to Peebles Grayle Tamayo for helping in the experiments done in the Philippines. This study was supported in part by a Grant-in-Aid for Scientific Research (24405044 to SK and 23689024 to YG) from the Japan Society for the Promotion of Science; and A-STEP Grant (AS232Z00111F to YG) from the Japan Science and Technology Agency; and Japan Society for the Promotion of Science Foreign Postdoctoral Grant (P14088).

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Authors and Affiliations

  1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan

    Kharleezelle J. Moendeg, Jose Ma. M. Angeles, Noboru Inoue & Shin-ichiro Kawazu

  2. Laboratory of Molecular Immunology, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    Yasuyuki Goto

  3. Department of Parasitology, College of Public Health, University of the Philippines, Manila, Philippines

    Lydia R. Leonardo, Elena A. Villacorte & Pilarita T. Rivera

  4. Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan

    Masashi Kirinoki & Yuichi Chigusa

Authors
  1. Kharleezelle J. Moendeg
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  2. Jose Ma. M. Angeles
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  3. Yasuyuki Goto
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  4. Lydia R. Leonardo
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  5. Masashi Kirinoki
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  6. Elena A. Villacorte
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  7. Pilarita T. Rivera
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  8. Noboru Inoue
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  9. Yuichi Chigusa
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  10. Shin-ichiro Kawazu
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Corresponding author

Correspondence to Shin-ichiro Kawazu.

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Moendeg, K.J., Angeles, J.M.M., Goto, Y. et al. Development and optimization of cocktail-ELISA for a unified surveillance of zoonotic schistosomiasis in multiple host species. Parasitol Res 114, 1225–1228 (2015). https://doi.org/10.1007/s00436-015-4312-7

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  • DOI: https://doi.org/10.1007/s00436-015-4312-7

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