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Temporal occurrence and community structure of helminth parasites in southern leopard frogs, Rana sphenocephala, from north central Oklahoma

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Abstract

Currently, little information is available about the temporal recruitment of helminth communities in amphibian hosts. We examined the helminth community structure and temporal recruitment of helminth parasites in southern leopard frogs, Rana sphenocephala. Specifically, we were interested in how host life history such as habitat, age and/or size, diet, sex, and temporal variation in abiotic factors (precipitation and temperature) were important in determining monthly infection patterns of helminth populations and communities in southern leopard frogs. From May to September 2011, 74 southern leopard frogs were collected from Teal Ridge in Stillwater Payne County, OK, USA. Sixty-nine (93 %) of 74 frogs were infected with 1 or more helminth species. During our collecting period, the average monthly temperature was lowest in May and highest in July, and monthly precipitation was highest in May and lowest during the first week of September. The component community consisted of 11 species of helminth, including 1 larval and 1 adult cestode, 2 larval and 3 adult trematodes, and 1 juvenile and 3 adult nematodes. Of the 1790 helminths recovered, 51 % (911) were nematodes, 47 % (842) were cestodes, and 2 % (37) were trematodes. There were significant differences in the total abundance and mean species richness of helminths acquired by skin contact or through frog diet in monthly component communities of southern leopard frogs. A positive correlation existed for percentage of all helminths acquired by skin contact and monthly precipitation (r = 0.94, P < 0.01). Conversely, a negative correlation existed for monthly precipitation and percentage of helminths acquired by diet (r = −0.94, P < 0.01). Our results indicate that abiotic conditions such as precipitation have a major influence on the avenues for and constraints on the transmission of helminths with life cycles associated with water/moisture or terrestrial intermediate/paratenic hosts and are important in structuring helminth communities of amphibian hosts.

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Acknowledgments

This research was conducted under the Oklahoma State University Institutional Animal Care and Use Committee protocol AS-10-2, and all animals were collected under the Oklahoma Department of Wildlife Conservation Special License numbers 5022 to MGB and 4752 to MSV. We thank two anonymous reviewers for improvements on this manuscript.

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  1. Department of Integrative Biology, Oklahoma State University, Stillwater, OK, 74078, USA

    M. Suhail Vhora & Matthew G. Bolek

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  1. M. Suhail Vhora
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  2. Matthew G. Bolek
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Correspondence to Matthew G. Bolek.

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Vhora, M.S., Bolek, M.G. Temporal occurrence and community structure of helminth parasites in southern leopard frogs, Rana sphenocephala, from north central Oklahoma. Parasitol Res 114, 1197–1206 (2015). https://doi.org/10.1007/s00436-014-4303-0

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