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Isoforms of Hsp70-binding human LDL in adult Schistosoma mansoni worms

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Abstract

Schistosoma mansoni is one of the most common parasites infecting humans. They are well adapted to the host, and this parasite’s longevity is a consequence of effective escape from the host immune system. In the blood circulation, lipoproteins not only help to conceal the worm from attack by host antibodies but also act as a source of lipids for S. mansoni. Previous SEM studies showed that the low-density lipoprotein (LDL) particles present on the surface of adult S. mansoni worms decreased in size when the incubation time increased. In this study, immunocytochemical and proteomic analyses were used to locate and identify S. mansoni binding proteins to human plasma LDL. Ultrathin sections of adult worms were cut transversely from the anterior, medial and posterior regions of the parasite. Immunocytochemical experiments revealed particles of gold in the tegument, muscle region and spine in male worms and around vitelline cells in females. Immunoblotting and 2D-electrophoresis using incubations with human serum, anti-LDL antibodies and anti-chicken IgG peroxidase conjugate were performed to identify LDL-binding proteins in S. mansoni. Analysis of the binding proteins using LC-MS identified two isoforms of the Hsp70 chaperone in S. mansoni. Hsp70 is involved in the interaction with apoB in the cytoplasm and its transport to the endoplasmic reticulum. However, further studies are needed to clarify the functional role of Hsp70 in S. mansoni, mainly related to the interaction with human LDL.

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Acknowledgments

This study received support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The authors would like to thank Ana Lúcia Oliveira Carvalho from the Mass Spectrometry and Proteomics Unit (UEMP), Federal University of Rio de Janeiro, Brazil, for the technical assistance.

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Laboratório de Expressão Gênica em Eucariotos, Instituto de Química - Departamento de Bioquímica, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, sala 1200, São Paulo, SP, 05508-000, Brazil

    Adriana S. A. Pereira

  2. Departamento de Fisiologia e Patologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba, Cidade Universitária, João Pessoa, PB, 58051-900, Brazil

    Marília G. S. Cavalcanti

  3. Unidade de Espectrometria de Massas e Proteômica (UEMP), Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, 21914-902, Brazil

    Russolina B. Zingali

  4. Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Professor Moraes Rego, s/n, Recife, PE, 50670-420, Brazil

    José L. Lima-Filho & Maria E. C. Chaves

  5. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Professor Moraes Rego, s/n, Recife, PE, 50670-901, Brazil

    José L. Lima-Filho & Maria E. C. Chaves

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  1. Adriana S. A. Pereira
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  2. Marília G. S. Cavalcanti
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  3. Russolina B. Zingali
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  4. José L. Lima-Filho
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Correspondence to Adriana S. A. Pereira.

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Pereira, A.S.A., Cavalcanti, M.G.S., Zingali, R.B. et al. Isoforms of Hsp70-binding human LDL in adult Schistosoma mansoni worms. Parasitol Res 114, 1145–1152 (2015). https://doi.org/10.1007/s00436-014-4292-z

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