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Morphological and phylogenetic analysis of Nosema sp. HR (Microsporidia, Nosematidae): a new microsporidian pathogen of Histia rhodope Cramer (Lepidoptera, Zygaenidae)

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Abstract

A new microsporidium was isolated from Histia rhodope Cramer (Lepidoptera, Zygaenidae), a pest of Bischofia javanica BL. in China. The morphology and molecular systematic of this novel microsporidian isolate had been described in this study. The spores were long oval and measured 3.1 × 1.9 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: 14–15 polar filament coils, posterior vacuole, and a diplokaryon. The sequenced rRNA gene of this isolate is 4309 bp long. The organization of the rRNA gene is 5′-LSU rRNA-ITS-SSU rRNA-IGS-5S-3′, which is similar to that of other Nosema species (such as Nosema bombycis). Phylogenetic analysis based on LSU rRNA gene and SSU rRNA gene both revealed that this novel micorsporidian which isolated from H. rhodope had close relationship to the genus Nosema. Additionally, this isolate can also cause systemic infection of Bombyx mori. So, we should pay attention not only to N. bombycis, but also to other microsporidian (such as Nosema sp. HR) in sericulture in the future.

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Acknowledgments

We thank all the authors for their free-charged software cited and used in this article. This work is supported by the Pre-research project of the National Natural Science Foundation of CQMU under Grant No. NSFYY201318 and National Natural Science Foundation of China under Grant No. 81270912.

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Authors and Affiliations

  1. Experimental Teaching Center, Chongqing Medical University, Yuzhong, Chongqing, 400016, People’s Republic of China

    Handeng Liu, Songtao Ding, Qizhong Qin, Jun Tang, Li Liu & Huimin Peng

  2. Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Handeng Liu, Songtao Ding & Huimin Peng

Authors
  1. Handeng Liu
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  2. Songtao Ding
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  3. Qizhong Qin
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  4. Jun Tang
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  5. Li Liu
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  6. Huimin Peng
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Corresponding author

Correspondence to Handeng Liu.

Additional information

Nucleotide sequence reported in this study has been submitted to the GenBank™, EMBL, and DDBJ databases under the accession number KP100640.

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Liu, H., Ding, S., Qin, Q. et al. Morphological and phylogenetic analysis of Nosema sp. HR (Microsporidia, Nosematidae): a new microsporidian pathogen of Histia rhodope Cramer (Lepidoptera, Zygaenidae). Parasitol Res 114, 983–988 (2015). https://doi.org/10.1007/s00436-014-4264-3

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  • DOI: https://doi.org/10.1007/s00436-014-4264-3

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