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Eimeria tenella: 14-3-3 protein interacts with telomerase

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Abstract

Telomerase, consisting of telomerase RNA and telomerase reverse transcriptase (TERT), is responsible for the maintenance of the end of linear chromosomes. TERT, as the catalytic subunit of telomerase, plays a critical role in telomerase activity. Researches indicate TERT-associated proteins participate in the regulation of telomerase assembly, posttranslational modification, localization, and enzymatic function. Here, the telomerase RNA-binding domain of Eimeria tenella TERT (EtTRBD) was cloned into pGBKT7 and performed as the bait. α-Galactosidase assay showed that the bait plasmid did not activate Gal4 reporter gene. Further, we isolated an EtTRBD-associated protein, 14-3-3, by yeast two-hybrid screening using the constructed bait plasmid. To confirm the interaction, EtTRBD and 14-3-3 were expressed by prokaryotic and eukaryotic expression systems. Pull-down assays by purified proteins demonstrated a direct bind between EtTRBD and 14-3-3. Co-immunoprecipitation techniques successfully validated that 14-3-3 interacted with EtTRBD in 293T cells. The protein-protein interaction provides a starting point for more in-depth studies on telomerase and telomere regulation in E. tenella.

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Acknowledgments

This work was supported by grants (nos. 31272550 and 30970322) from the National Natural Science Foundation of China (NSFC). The authors declare that the experiments comply with the current laws of China

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, China

    Na Zhao, Pengtao Gong, Jianhua Li, Zhengtao Yang, He Li, Ju Yang, Guocai Zhang & Xichen Zhang

  2. College of Life Science, Jilin University, 2699 Qianjin Street, Changchun, 130012, China

    Baiqi Cheng

Authors
  1. Na Zhao
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  2. Pengtao Gong
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  3. Baiqi Cheng
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  4. Jianhua Li
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  5. Zhengtao Yang
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  6. He Li
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  7. Ju Yang
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  8. Guocai Zhang
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  9. Xichen Zhang
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Corresponding authors

Correspondence to Jianhua Li or Xichen Zhang.

Additional information

Na Zhao and Pengtao Gong contributed equally to this study.

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Zhao, N., Gong, P., Cheng, B. et al. Eimeria tenella: 14-3-3 protein interacts with telomerase. Parasitol Res 113, 3885–3889 (2014). https://doi.org/10.1007/s00436-014-4108-1

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  • DOI: https://doi.org/10.1007/s00436-014-4108-1

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