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Zanthoxylum caribaeum (Rutaceae) essential oil: chemical investigation and biological effects on Rhodnius prolixus nymph

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Abstract

A chemical investigation and bioassays against fifth-instar nymphae of the hematophagous insect Rhodnius prolixus, vector of Chagas disease, were conducted with the essential oil from Zanthoxylum caribaeum. The main results may be summarized as follows: (i) 54 components were identified, corresponding to 90.4 % of the relative composition; sesquiterpenes (47.3 %) and monoterpenes (41.2 %) are the major constituents; (ii) muurola-4,5-trans-diene and isodaucene are described for the first time as chemical constituents of the essential oil from leaves of this species; (iii) topical treatment with the crude essential oil induced high levels of paralysis (from 18.88 to 33.33 %) and mortality (from 80 to 98.9 %) depending on the dose applied (0.5 to 5.0 μl per insect); (iv) feeding treatment with the crude essential oil also induced high levels of mortality (from 48.8 to 100 %) but low levels of paralysis (from 2.22 to 7.77 %) depending on the dose applied (0.5 to 5.0 μl/ml of blood); (v) in the continuous treatment, only the dose of 5.0 μl/cm2 was able to promote statistical significant levels of mortality (63.3 %) but no paralysis were detected. However in this group, occasionally, only few insects displayed malformations of legs and wings after treatment; and (vi) any treatment was able to disrupt the metamorphosis process since the low adult stage emergence observed to all groups was due the high insect mortality. These observations suggest the interference of Z. caribaeum compounds on the triatomine neuroendocrine system. The significance of these results in relation to the relevant biological events in R. prolixus as well as the possible use of insect growth regulators present in Z. caribaeum oil in integrated vector control programs against hematophagous triatomine species is herein discussed.

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Acknowledgments

We thank Universidade Federal Fluminense for the grants to the scientific initiation students as well as Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Apoio à Pesquisa do Estado do Rio de Janeiro (FAPERJ), and PROCIENCIA-Universidade do Estado do Rio de Janeiro for financial support. We also thank José Rubens Pirani (Universidade de São Paulo) for the confirmation of species identification and Genilton José Vieira and Heloisa Maria Nogueira Diniz (Laboratório de Produção e Tratamento de Imagens, Instituto Oswaldo Cruz/FIOCRUZ) for helping with the illustrations.

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Authors and Affiliations

  1. Laboratório de Tecnologia de Produtos Naturais, Universidade Federal Fluminense, Rua Doutor Mário Viana 523, Santa Rosa, 24241-000, Niterói, RJ, Brazil

    J. Nogueira & L. Rocha

  2. Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Doutor Mário Viana 523, Santa Rosa, 24241-000, Niterói, RJ, Brazil

    S. C. Mourão

  3. Laboratório de Farmacotécnica, Colegiado de Ciências Farmacêuticas, Universidade Federal do Amapá, Amapá, AP, Brazil

    C. P. Fernandes

  4. Laboratório de Produtos Naturais do Mar e de Química Bio-Orgânica, Departamento de Biologia Geral, Universidade Federal Fluminense, Campus Valonguinho, Centro, 24210-130, Niterói, RJ, Brazil

    A. Kelecom

  5. Laboratório de Biologia de Insetos, Departamento de Biologia Geral, Universidade Federal Fluminense, Campus Valonguinho, Centro, 24210-130, Niterói, RJ, Brazil

    I. B. Dolabela, C. B. Mello & D. Feder

  6. Departamento de Ciências, Faculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro, Rua Dr. Francisco Portela, 1.470, Patronato, 24435-005, São Gonçalo, RJ, Brazil

    M. G. Santos

  7. Laboratório de Produção e Tratamento de Imagens, Fiocruz, Instituto Oswaldo Cruz /IOC /FIOCRUZ, Av. Brasil, 4365, Manguinhos, 21040-360, Rio de Janeiro, RJ, Brazil

    R. Mexas

  8. Laboratório de Biologia de Insetos (GBG-UFF), Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq), Outeiro São João Batista s/número-Centro- Niterói- Campus do Valonguinho (UFF), 24020-141, Niterói, RJ, Brazil

    M. S. Gonzalez

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  1. J. Nogueira
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Nogueira, J., Mourão, S.C., Dolabela, I.B. et al. Zanthoxylum caribaeum (Rutaceae) essential oil: chemical investigation and biological effects on Rhodnius prolixus nymph. Parasitol Res 113, 4271–4279 (2014). https://doi.org/10.1007/s00436-014-4105-4

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