Abstract
The structure modulation of follicular cells and the ovarian changes during fourth larval instar and pupal stage of the malaria vector mosquitoes Anopheles pharoensis Theobald were investigated using the light and electron microscopy. The generative organs consist of a pair of polytrophic ovaries (OV), which are oblong, spindle-shaped bodies, lying dorsolaterally and occupying the region from the mid-fifth to the mid-sixth abdominal segment in the fourth larval instar, while in the pupal stage, each ovary (OV) is situated in the haemocoel of the sixth abdominal segment. It is an oblong body slightly larger in diameter; the lumen of the calyx becomes wider and central, and the pedicel (P) consists of one row of compact discoidal cells; meanwhile, in the fourth larval instar, the pedicel is without a lumen and consists of two rows of discoidal cells which are arranged as a short column between the follicle and calyx. The mean volume of the follicle in the fourth larval instar is 9.078 ± 3.0178 μm3, meanwhile in the pupal stage being 12.051 ± 2.427 μm3. The germarium (G) decreases in size in the pupal stage and contains a group of cells from which the oogonia differentiate, follicular cells which are similar to trophocytes, undifferentiated into one oocyte (O), which will develop into an egg and it is statistically the smallest one measured (0.058 ± 0.0041 μm3, 0.303 ± 0.0086 μm3) in fourth larval instar and pupal stage, respectively as compared to the others within the follicle which will be accompanied as nurse cells (NC). The follicle is enclosed by a mononuclear flattened cells (follicular membrane), which have distinct boundaries. The vitellarium is differentiated into primary (F1) and secondary follicles (F2) in the pupal stage. The Golgi apparatus (GA) appears as discrete bits which are restricted to the perinuclear zone. The mitochondria (M) in the fourth larval instar are in the form of granules and short rods. They are perinuclearly distributed, forming a ring that surrounds the comparatively large nucleus. In the pupal stage, a similar condition to that described for the larva is observed, but with an increase in size and numbers, due to breaking up of rods into granules.
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Adham FK, Mehlhorn H, El-Basheir ZM, Yamany AS (2009) Light and electron microscopic studies on the development of the ovaries of Culex pipiens quinquefasciatus (Say) (Diptera: Culicidae). Parasitol Res 105(4):939–948
Ahmed SA (1983) Cytological and cytochemical studies in Culex pipiens molestus (Forskal). Oocyte differentiation and vitellogenesis. M Sc Thesis Fac Science Alexandria University
Beklemishev WN (1944) Ecology of the malaria. Medgiz, Moscow
Bellamy RE, Bracken GK (1971) Quantitative aspects of ovarian development in mosquitoes. Can Entomol 103:763–773
Briegel H (1990a) Fecundity, metabolism, and body size in Anopheles (Diptera: Culicidae), vectors of malaria. J Med Entomol 27:839–850
Briegel H (1990b) Metabolic relationship between female body size, reserves, and fecundity in Ae. aegypti. J Insect Physiol 36:165–172
Carnevale P, Molinier M, Bosseno MF and Mouchet J (1978) Relations mathématiques dans la maturation des follicles ovariens des females pares d’ An. gambiae (Diptera: Culicidae). Cahier O. R. S. T. O. M. série Entomologie Médicale et Parasitologie 16: 121–127
Christophers’ SR (1911) The development of the egg follicle in anophelines. Paludism 2:73–88
Christophers’ SR (1923) The structure and development of the female genital organs and hypopygium of the mosquito. Indian J Med Res X:698–720
Christophers’ SR (1960) Ae. aegypti (L.), the yellow fever mosquito; its life history bionomics, and structure. Cambridge University Press 738pp
Clements AN (1963) The physiology of mosquitoes. The Macmillan Co., New York, 393p
Clements AN, Boocock MR (1984) Ovarian development in mosquitoes: stages of growth and arrest, and follicular resorption. Shell Research Limited, Sittingbourne Research Centre, Sittingbourne, pp 1–8
Detinova TS (1949) Physiological changes of ovaries in females of An. maculipennis. Med Parazitol (Mosq) 18:410
Detinova TS (1962) Age-grouping methods in Diptera of medical importance. WHO Geneva, Switzerland. 47: 13
Kawai S (1969) Studies on the follicular development and feeding activity of the females of Culex tritaeniorhynchus with special reference to those in autumn. Trop Med (Nagasaki) 11:145–169
King RC, Rubinson AC, Smith RF (1956) Oogenesis in adult Drosophila melanogaster. Growth 20:121–157
Lea AO, Briegel H, Lea HM (1978) Arrest, resorption, or maturation of oocytes in Aedes aegypti (Lin.). A dependence on the quantity of blood and the interval between blood meals. Physiol Entomol 3:309–316
Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–410
Macan TT (1950) The anopheline mosquitoes of Iraq and North Persia. Mem Lond School Hyg Trop Med 7:109–122
Nath VM (1924) Egg-follicle of Culex. Q J Microsc Sci 65:152–175
Nicholson AJ (1921) The development of the ovary and ovarian egg of a mosquito, Anopheles maculipennis. Q J Microsc Sci 65:395–448
Polovodova VP (1949) Age changes in the female reproductive system of Anopheles and age composition of mosquito populations, Rostov-on-Don (Thesis)
Reyes F, Villanueva (2004) Egg development may require multiple bloodmeals among small Aedes aegypti (Diptera: Culicidae) field collected in northeastern Mexico. 87(4): 630–632
Ronquillo MC, Horsfall WR (1969) Genesis of the reproductive system of mosquitoes. I. Female of Aedes stimulans (Walker). J Morphol 129(3):249–270
Roth TF, Porter KR (1964) Yolk protein uptake in the oocyte of the mosquito Aedes aegypti (L.). J Cell Biol 20:313–332
Telang A, Wells MA (2004) The effect of larval and adult nutrition on successful autogenous egg production by a mosquito. J Insect Physiol 50:677–685
Volozina NV (1967) The effect of the amount of blood engorged and of supplementary carbohydrate feeding on the process of oogenesis in the females of blood sucking mosquitoes in the genus Aedes (Diptera: Culicidae) of different weights and ages. Entomol Oboze 46:49–59
Watts RB, Smith SM (1978) Oogenesis in Toxorhynchites rutilus (Diptera: Culicidae). Can J Zool 56:136–139
Woke PA (1937) Comparative effects of the blood of different species of vertebrates on egg-production of Aedes aegypti (Linn.). Am J Trop Med 17:729–745
Yaguzhinskaya LV (1945) The morphology of the blood clot and the chemical changes in the blood pigment at different stages of digestion in the female of Anopheles maculipennis. Med Parazitol (Mosk) 14:38–45
Yamany AS (2012) Studies on the development of the ovaries of the malaria mosquitoes (Anopheles pharoensis). J Vaccines Vaccin 3(2):1–6
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Yamany, A.S., Adham, F.K. & Mehlhorn, H. Structural changes of the follicular cells during developmental stages of the malaria vector mosquitoes Anopheles pharoensis (Diptera: Culicidae) in Egypt. Parasitol Res 113, 4233–4241 (2014). https://doi.org/10.1007/s00436-014-4099-y
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DOI: https://doi.org/10.1007/s00436-014-4099-y