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Antimalarial efficacy of dynamic compound of plumbagin chemical constituent from Plumbago zeylanica Linn (Plumbaginaceae) against the malarial vector Anopheles stephensi Liston (Diptera: Culicidae)

Parasitology Research volume 113, pages 3105–3109 (2014)Cite this article

Abstract

In the present investigation, the effective root compound of plumbagin of Plumbago zeylanica (Plumbaginaceae) was evaluated for chemical constituent and antimalarial effect against the fourth instar larvae of Anopheles stephensi Liston (Diptera). In the chromatographic analyses of root compound with Rf value of 0.788 and NMR analyses also revealed that the effective compound contain naphthoquinone plumbagin were identified as the major chemical constituent. Larval mortality was observed after 3 h of exposure period. The plumbagin compound showed remarkable larvicidal activity against A. stephensi (LC50 32.65 and LC9072.27 ppm). Histopathological effects of compound was observed in the treated larvae. Based on the results, the plumbagin compound of P. zeylanica can be considered as a new source of natural larvicide for the control of malarial vector.

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References

  • Amer A, Mehlhorn H (2006a) Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473–477

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006b) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006c) Repellency effect of forty-one essential oils against Aedes, Anopheles and Culex mosquitoes. Parasitol Res 99:478–490

    Article  PubMed  Google Scholar 

  • Arunachalam KD (2010) Anti-inflammatory and cytotoxic effects of extract from Plumbago zeylanica. Afr J Microbiol Res 4(12):1239–1245

    Google Scholar 

  • Dai Y, Hou L, Chan Y, Cheng L, But PP (2004) Inhibition of immediate allergic reactions by ethanol extract from Plumbago zeylanica stems. Biol Pharmacol Bull 27(3):429–432

    Article  CAS  Google Scholar 

  • Jiangsu (1979) New Medical College. Zhonyao Dictionary (Encyclopedia of Chinese Materia). Scientific and Technological Press, Shanghai, pp 711–712

    Google Scholar 

  • Jeyachandran R, Mahesh A, Cindrella L, Sudhakar S, Pazhanichamy K (2009) Antibacterial activity of Plumbagin and root extracts of Plumbago zeylanica. Acta Biol Cracoviensia Ser Botan 51(1):17–22

    Google Scholar 

  • Kalaivani K, Senthil-Nathan S, Murugesan AG (2012) Biological activity of selected Lamiaceae and Zingiberaceae plant essential oils against the dengue vector Aedes aegypti L. (Diptera: Culicidae). Parasitol Res 110:1261–1268

  • Kannathasan K, Senthilkumar A, Venkatesalu V (2011) Mosquito larvicidal activity of methyl-p-hydroxybenzoate isolated from the leaves of Vitex trifolia Linn. Acta Trop 120:115–118

    Article  CAS  PubMed  Google Scholar 

  • Lin LC, Yang LL, Chou CJ (2003) Cytotoxic naphthoquinones and plumbagic acid glucosides from Plumbago zeylanica. Phytochem 62:619–622

    Article  CAS  Google Scholar 

  • Maniafu BM, Wilber L, Ndiege IO, Wanjala CCT, Akenga TA (2009) Larvicidal activity of extracts from three Plumbago spp against Anopheles gambiae. Mem Inst Oswaldo Cruz 104:813–817

    Article  CAS  PubMed  Google Scholar 

  • Olagunju JA, Fagbohunka BS, Oyedapo OO, Abdul AIA (2006) Effects of an ethanolic root extract of Plumbago zeylanica Linn. on some serum parameters of the rats. RPMP-Drug Dev Mol 11:268–276

    Google Scholar 

  • Patil SV, Patil CD, Salunkhe RB, Salunke BK (2010) Larvicidal activities of six plants extracts against two mosquito species, Aedes aegypti and Anopheles stephensi. Trop Biomed 27(3):360–365

    CAS  PubMed  Google Scholar 

  • Senthil-Nathan S (2007) The use of Eucalyptus leaf extract as a natural larvicidal agent against malarial vector Anopheles stephensi Liston (Diptera: Culicidae). Bioresour Technol 98 (9):1856–1860

  • Senthil-Nathan S (2013) Physiological and biochemical effect of Neem and other Meliaceae plants secondary metabolites against Lepidopteran insects. Front Invert Physiol 4 (359):1–17

  • Senthil-Nathan S, Kalaivani K, Murugan K, Chung PG (2005) Effects of neem limonoids on malarial vector Anopheles stephensi Liston (Diptera: Culicidae). Acta Tropica 96(1):47–55

  • Senthil-Nathan S, Kalaivani K, Sehoon K (2006) Effects of Dysoxylum malabaricum Bedd. (Meliaceae) extract on the malarial vector Anopheles stephensi Liston (Diptera: Culicidaae). Bioresour Technol 97(16):2077–2083

    Article  PubMed  Google Scholar 

  • Senthilkumar N, Verma P, Gurusubamanian G (2009) Larvicidal and adulticidal activities of some medicinal plants against the malarial vector Anopheles stephensi (Liston). Parasitol Res 104:237–244

    Article  CAS  PubMed  Google Scholar 

  • Silverstein RM, Webster FX, Kiemle DJ (2005) Proton NMR spectrometry. Spectrometric identification of organic compounds. John Wiley and Sons, Inc, USA, pp 127–204

    Google Scholar 

  • Singh M, Nagori K, Iyer S, Khare G, Sharwan G, Tripathi DK (2011) Ethnomedicinal, traditional and pharmacological aspects of Plumbago zeylanica Linn. Pharmacol Online 3:684–700

    Google Scholar 

  • Thanigaivel A, Chandrasekaran R, Revathi K, Nisha S, Sathish-Narayanan S, Kirubakaran SA, Senthil-Nathan S (2012) Larvicidal efficacy of Adhatoda vasica (L.) Nees against the bancroftian filariasis vector Culex quinquefasciatus Say and dengue vector Aedes aegypti L. in vitro condition. Parasitol Res 110:1993–1999

  • Tilak JC, Adhikari S, Devasagayam TP (2004) Antioxidant properties of Plumbago zeylanica, an Indian medicinal plant and its active ingredient, plumbagin. Redox Rep 9:219–227

    Article  CAS  PubMed  Google Scholar 

  • Vogel A (1978) A text book of quantitative inorganic analysis including instrumental analysis. Longman, London and New York, pp 69–74

    Google Scholar 

  • World Health Organisation (1981) Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO-VBC 81.807, pp. 1–6

  • World Health Organization (1992) Vector resistance to pesticides. Fifteenth report of the WHO expert committee on vector biology and control. WHO technical report, Geneva

    Google Scholar 

  • World Health Organization (2013) World malaria report. WHO, National Press Club in Washington

  • Yang YC, Lee SG, Lee HK, Kim MK, Lee SH, Lee HS (2002) A piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae. J Agric Food Chem 50:3765–3767

    Article  CAS  PubMed  Google Scholar 

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Funding sources

Department of Biotechnology (DBT), Government of India (PR13126/GBD/27/194/2009).

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Authors and Affiliations

  1. Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi – 627 412, Tirunelveli, Tamil Nadu, India

    Venkatraman Pradeepa, Subbiah Sathish-Narayanan, Suyambulingam Arunachalam Kirubakaran & Sengottayan Senthil-Nathan

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  1. Venkatraman Pradeepa
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  2. Subbiah Sathish-Narayanan
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  3. Suyambulingam Arunachalam Kirubakaran
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  4. Sengottayan Senthil-Nathan
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Correspondence to Sengottayan Senthil-Nathan.

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Pradeepa, V., Sathish-Narayanan, S., Kirubakaran, S.A. et al. Antimalarial efficacy of dynamic compound of plumbagin chemical constituent from Plumbago zeylanica Linn (Plumbaginaceae) against the malarial vector Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 113, 3105–3109 (2014). https://doi.org/10.1007/s00436-014-4015-5

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  • DOI: https://doi.org/10.1007/s00436-014-4015-5

Keywords

  • Mosquito vector
  • Medicinal plant
  • NMR
  • Larvicide
  • Histopathology