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Parasitology Research

, Volume 113, Issue 9, pp 3195–3199 | Cite as

Aedes japonicus japonicus (Diptera: Culicidae) from Germany have vector competence for Japan encephalitis virus but are refractory to infection with West Nile virus

  • Katrin Huber
  • Stephanie Jansen
  • Mayke Leggewie
  • Marlis Badusche
  • Jonas Schmidt-Chanasit
  • Norbert Becker
  • Egbert Tannich
  • Stefanie C. Becker
Original Paper

Abstract

The interplay between arthropod-borne (arbo) viruses and their vectors is usually complex and often exert unique relationships. Aedes japonicus japonicus (Hulecoeteomyia japonica or Ochlerotatus japonicus japonicus), an invasive mosquito species with laboratory proven vector competence for a number of emerging viruses has been newly introduced to Germany and is currently expanding its range throughout the country. On the other hand, West Nile virus (WNV), an emerging arbovirus originating from Africa, is already circulating in several European countries and might soon be introduced to Germany. Because newly introduced and rapidly expanding vector species pose a potential risk for public health in Germany, we assessed the vectorial capacity of German Ae. j. japonicus populations for WNV and Japanese encephalitis virus (JEV). The results indicate that German Ae. j. japonicus are susceptible for JEV but are refractory to infection with WNV. Of 67 Ae. j. japonicus females challenged by feeding of WNV-containing blood, none had measurable amounts of WNV-RNA (0 % infection rate) on day 14 post-infection. In contrast, all females challenged with JEV were positive for JEV-RNA (100 % infection rate) on day 14 post-infection. The reason for WNV resistance remains to be determined but is independent from co-infection with other flaviviruses or the presence of endosymbiotic Wolbachia, since we found no evidence for other flavivirus infections within 1,033 tested A. j. japonicus females from the sampling region, nor detectable Wolbachia infection within 30 randomly selected individuals.

Keywords

Aedes japonicus japonicus Vector competence West Nile virus 

Notes

Acknowledgments

This work was financially supported by the Leibniz Association, grant number SAW-2011-BNI-3 and the German Federal Ministry for Environment, Nature Conservation, Building and Nuclear Safety (BMUB) through the Federal Environment Agency (UBA), grant number FKZ371148404. We thank Andreas Krüger for the critical reading of the manuscript.

Supplementary material

436_2014_3983_Fig1_ESM.gif (110 kb)
Fig. S1

Quality control of RNAs used for WNV detection by 28S RT-PCT. RNA isolates from single WNV infection experiments (4 mosquitoes/experiment) were subjected to a 28S RNA RT-PCR. PCR products are separated by agarose gel. The gel shows the PCR products for the single specimens in lanes 1–29 and a 1 kb ladder in lane L. All RNAs tested yielded the expected 250 bp fragment

436_2014_3983_MOESM1_ESM.tiff (2.1 mb)
High resolution image

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Katrin Huber
    • 1
    • 2
    • 3
  • Stephanie Jansen
    • 1
  • Mayke Leggewie
    • 1
  • Marlis Badusche
    • 1
  • Jonas Schmidt-Chanasit
    • 1
  • Norbert Becker
    • 2
  • Egbert Tannich
    • 1
    • 4
  • Stefanie C. Becker
    • 5
  1. 1.Bernhard Nocht Institute for Tropical MedicineHamburgGermany
  2. 2.German Mosquito Control Association (KABS/GFS)WaldseeGermany
  3. 3.University of HeidelbergHeidelbergGermany
  4. 4.German Centre for Infection Resarch, partner site Hamburg-Luebeck-BorstelHamburgGermany
  5. 5.Research Group EntomologyBernhard Nocht Institute for Tropical Medicine HamburgHamburgGermany

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