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Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis

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Abstract

Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5 % and DEAE S2 90 %. The best specificity was observed for DEAE S2 (90.4 %). In IB, using DEAE S2 samples from NC patients presented bands of 20–25, 43–45, 55–50, 60–66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.

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References

  • Assunção FG, Gleyce AM, Heliana BO, Maria TN, Pacheco R, José RM, Julia MC (2010) Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis. Rev Soc Bras Med Trop 43:254–259

    Article  Google Scholar 

  • Atluri SR, Singhi P, Khandelwal N, Malla N (2009) Neurocysticercosis immunodiagnosis using Taenia solium cysticerci crude soluble extract, excretory secretory and lower molecular mass antigens in serum and urine samples of Indian children. Acta Trop 110:22–27

    Article  PubMed  Google Scholar 

  • Baermann G (1917) Eine einfache Methode zur Auffinfung von Ankylostomun (Nematoden) Larven in Erdproben. Mededeel. mit H. Geneesk.Batavia, Lababoratories Weltevreden Feestbundel 41–47.

  • Barcelos IS, Mineo JR, Silva DAO, Ferreira MS, de Moura LP, Biondi GF, Costa-Cruz JM (2001) Detection of IgG in cerebrospinal fluid for diagnosis of neurocysticercosis: evaluation of saline and SDS extracts from Taenia solium and Taenia crassiceps metacestodes by ELISA and immunoblot assay. Trop Med Int Health 6:219–226

    Article  CAS  PubMed  Google Scholar 

  • Barcelos IS, Moura LP, Costa VP, Ferreira MS, Costa-Cruz JM (2007) Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis. Mem Inst Oswaldo Cruz 102:713–717

    Article  CAS  PubMed  Google Scholar 

  • Bueno EC, Vaz AJ, Machado LR, Livramento JA, Mielle SR (2000a) Specific Taenia crassiceps and Taenia solium antigenic peptides for neurocysticercosis immunodiagnosis using serum samples. J Clin Microbiol 38:146–151

    CAS  PubMed Central  PubMed  Google Scholar 

  • Bueno EC, Vaz AJ, Machado LR, Livramento JA (2000b) Detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens. Arq Neuropsiquiat 58:18–24

    Article  CAS  PubMed  Google Scholar 

  • Costa JM (1986) Immunoenzymatic test (ELISA) in the diagnosis of neurocysticercosis: study of various antigenic extracts in the detection of IgG antibodies in serum and cerebrospinal fluid samples. Arq Neuropsiquatr 44:15–31

    CAS  Google Scholar 

  • Costa JM, Ferreira AW, Makino MM, Camargo ME (1982) Spinal fluid immunoenzymatic assay (ELISA) for neurocysticercosis. Rev Inst Med Trop Sao Paulo 24:337–341

    CAS  PubMed  Google Scholar 

  • Cummins PM, Dowling O, O’Connor BF (2011) Ion-exchange chromatography: basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase. Chromat Meth Mol Biol 681:215–228

    Article  CAS  Google Scholar 

  • Del Brutto OH (2012) Diagnostic criteria for neurocysticercosis, revisited. Pathog Glob Health 106:299–304

    Article  PubMed  Google Scholar 

  • Escobar A, Aruffo C, Cruz-Sanchez F, Cervos-Navarro J (1985) Neuropathologic findings in neurocysticercosis. Arch Neurobiol 48:151–156

    CAS  Google Scholar 

  • Feliciano ND, Gonzaga HT, Gonçalves-Pires MRF, Gonçalves AL, Rodrigues RM, Ueta MT, Costa-Cruz JM (2010) Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis. Diagn Microbiol Infect Dis 67:153–161

    Article  PubMed  Google Scholar 

  • Friedman RD (1982) Comparison of four different silver-staining techniques for salivary protein detection in alkaline poly-acrilamyde gels. Anal Biochem 126:346–349

    Article  CAS  PubMed  Google Scholar 

  • Garcia HH, Rodriguez S, Gilman RH, Gonzalez AE, Tsang VC, Cysticercosis Working Group in Peru (2012) Neurocysticercosis: is serology useful in the absence of brain imaging? Trop Med Int Health 17:1014–1018

    Article  Google Scholar 

  • Gonzaga HT, Vila-Verde C, Nunes DS, Ribeiro VS, Cunha-Júnior JP, Costa-Cruz JM (2013) Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis. Parasitology 40:69–75

    Article  Google Scholar 

  • Gottstein B, Tsang VCW, Schantz PM (1986) Demonstration of species-specific and cross-reactive components of Taenia solium metacestode antigens. Am J Trop Med Hyg 35:308–313

    CAS  PubMed  Google Scholar 

  • Greiner M, Sohr D, Göbel P (1995) A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests. J Immunol Methods 185:123–132

    Article  CAS  PubMed  Google Scholar 

  • Hancock K, Pattabhi S, Whitfield FW (2006) Characterization and cloning of T24, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasit 147:109–117

    Article  CAS  Google Scholar 

  • Hanley JA, Macneil BJ (1982) The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology 143:29–36

    CAS  PubMed  Google Scholar 

  • Hanley JA, Mcneil BJ (1983) A method of comparing the áreas under receiver operating characteristic curves derived from the same cases. Radiology 148:839–843

    CAS  PubMed  Google Scholar 

  • Ishida MM, Rubinsky-Elefant G, Ferreira AW, Hoshino-Shimizu S, Vaz AJ (2003) Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Trop 89:73–84

    Article  CAS  PubMed  Google Scholar 

  • Ishida MM, Peralta RH, Livramento JA, Hoshino-Shimizu S, Peralta JM, Vaz AJ (2006) Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay. Rev Inst Med Trop Sao Paulo 48:343–346

    Article  PubMed  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 29:265–275

    Google Scholar 

  • Lutz A (1919) O Schistosomun mansoni e a schistosomatose segundo observações feitas no Brasil. Mem Inst Oswaldo Cruz 11:121–155

    Article  Google Scholar 

  • Machado GA, Santiago FM, Mineo JR, Costa-Cruz JM (2007) Assessment of antigenic fractions of varying hydrophobicity from Taenia solium metacestodes for the diagnosis of human neurocysticercosis. Trop Med Int Health 12:1369–1376

    Article  CAS  PubMed  Google Scholar 

  • Machado GA, Oliveira HB, Gennari-Cardoso ML, Mineo JR, Costa-Cruz JM (2013) Serodiagnosis of human neurocysticercosis using antigenic components of Taenia solium metacestodes derived from the unbound fraction from jacalin affinity chromatography. Mem Inst Oswaldo Cruz 108(3):368–375

    Article  CAS  PubMed Central  Google Scholar 

  • Martinez EZ, Louzada-Neto F, Pereira BB (2003) A curva ROC para testes diagnósticos. Cad Saúde Col 11:7–31

    Google Scholar 

  • Nunes DS, Gonzaga HT, Ribeiro VS, Cunha-Junior JP, Costa-Cruz JM (2013) Taenia saginata metacestode antigenic fractions obtained by ion-exchange chromatography: potential source of immunodominant markers applicable in the immunodiagnosis of human neurocysticercosis. Diagn Microbiol Infect Dis 76:36–41

    Article  Google Scholar 

  • Nunes DS, Ribeiro VS, Manhani MN, Costa-Cruz JM (2010) Jacalin-unbound fraction of Taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid. Diagn Microbiol Infect Dis 68:259–264

    Article  CAS  Google Scholar 

  • Oliveira HB, Machado GA, Mineo JR, Costa-Cruz JM (2010) Taenia saginata metacestode antigenic fractions without affinity to Concanavalin A are an important source of specific antigens for the diagnosis of human neurocysticercosis. Clin Vaccine Immunol 17:638–644

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Pardini AX, Peralta RH, Vaz AJ, Machado LR, Peralta JM (2002) Use of Taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (Taenia solium). Clin Diagn Lab Immunol 9:190–193

    CAS  PubMed Central  PubMed  Google Scholar 

  • Peralta RH, Vaz AJ, Pardini A, Macedo HW, Machado LR, De Simone SG, Peralta JM (2002) Evaluation of an antigen from Taenia crassiceps cysticercus for the serodiagnosis of neurocysticercosis. Acta Trop 83:159–168

    Article  CAS  PubMed  Google Scholar 

  • Rajkotia Y, Lescano AG, Gilman RH, Cornejo C, Garcia HH (2007) Economic burden of neurocysticercosis: results from Peru. Trans R Soc Trop Med Hyg 101:840–846

    Article  PubMed  Google Scholar 

  • Rajshekhar V, Oommen A (1997) Serological studies using ELISA and EITB in patients with solitary cysticercus granuloma and seizures. Neurol Infect Epidemiol 2:177–180

    Google Scholar 

  • Ribeiro VS, Manhani MN, Costa-Cruz JM (2010) IgA detection in human neurocysticercosis using different preparations of heterologous antigen. Parasitol Res 107:221–225

    Article  Google Scholar 

  • Sahu PS, Parija SC, Jayachandran S (2010) Antibody specific to 43 kDa excretory–secretory antigenic peptide of Taenia solium metacestode as a potential diagnostic marker in human neurocysticercosis. Acta Trop 115:257–261

    Article  CAS  PubMed  Google Scholar 

  • Sako Y, Itoh S, Okamoto M, Nakaya K, Ito A (2013) Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis. Parasitology 21:1–6

    Google Scholar 

  • Shiguekawa KY, Mineo JR, de Moura LP, Costa-Cruz JM (2000) ELISA and western blotting tests in the detection of IgG antibodies to Taenia solium metacestodes in serum samples in human neurocysticercosis. Trop Med Int Health 5:443–449

    Article  CAS  PubMed  Google Scholar 

  • Silva ADT, Quagliato EMAB, Rossi CL (2000) A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis using a purified fraction from Taenia solium cysticerci. Diagn Microbiol Infect Dis 37:87–92

    Article  PubMed  Google Scholar 

  • Suzuki LA, Rossi CL (2011) Evaluation of two Taenia solium cysticercal antigenic preparations (vesicular fluid and a glycoprotein fraction with affinity for lentil lectin) for the immunodiagnosis of neurocysticercosis by enzyme-linked immunosorbent assay (ELISA). Arq Neuropsiquiatr 69:470–474

    Article  PubMed  Google Scholar 

  • Tsang VC, Brand JA, Boyer AE (1989) An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159:50–59

    Article  CAS  PubMed  Google Scholar 

  • Youden WJ (1950) Index for rating diagnostic tests. Cancer 3:32–35

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

Financial support

This study was supported by the Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), and the Federal University of Uberlândia (UFU), Brazil.

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  1. Departamento de Imunologia, Microbiologia e Parasitologia Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil

    Vanessa da S. Ribeiro, Daniela da S. Nunes, Henrique T. Gonzaga, Jair P. da Cunha-Junior & Julia M. Costa-Cruz

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  1. Vanessa da S. Ribeiro
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  2. Daniela da S. Nunes
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  3. Henrique T. Gonzaga
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  4. Jair P. da Cunha-Junior
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  5. Julia M. Costa-Cruz
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Correspondence to Julia M. Costa-Cruz.

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da S. Ribeiro, V., da S. Nunes, D., Gonzaga, H.T. et al. Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis. Parasitol Res 113, 2569–2575 (2014). https://doi.org/10.1007/s00436-014-3908-7

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  • DOI: https://doi.org/10.1007/s00436-014-3908-7

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