Abstract
To enhance the anti-coccidial effect of dinitolmide and reduce its residual, the dinitolmide/MMT compounds were synthesized by the method of solution intercalation via dinitolmide intercalated into Na + -montmorillonite (Na + -MMT). The structure of compounds was characterized by X-ray diffraction and Fourier transformed infrared. Its anti-coccidial effect was examined by Eimeria tenella infection experiment. One hundred fifty AA broiler chickens were divided into five groups. Chickens were orally inoculated with 5 × 104 E. tenella oocysts after dinitolmide was given. Their curative effects were observed. The results showed that intercalated dinitolmide expanded the basal spacing (d 001) of MMT from 12.6 to 15.2 Å. The IR bands of amide group in dinitolmide/MMT were detected at 1,533 cm−1 which showed that dinitolmide was successfully intercalated into the interlayer spaces of MMT. The dinitolmide/MMT showed higher anti-coccidian oocyst activity compared with dinitolmide (p < 0.05). The dinitolmide/MMT compound can significantly increase body weight gains and reduce bloody diarrhea, lesion score, and oocyst excretion. The anti-coccidia index of dinitolmide/MMT group (165.21) is much higher than dinitolmide group (88.84). The dinitolmide/MMT hybrid systems can be more effective in control of coccidiosis in comparison to dinitolmide.
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References
Amarasinghe PM, Katti KS, Katti DR (2009) Nature of organic fluid-montmorillonite interactions: an FTIR spectroscopic study. J Colloid Interface Sci 337(1):97–105. doi:10.1016/j.jcis.2009.05.011
Awad AM, El-Nahas AF, Abu-Akkada SS (2013) Evaluation of the protective efficacy of the anticoccidial vaccine Coccivac-B in broilers, when challenged with Egyptian field isolates of E. tenella. Parasitol Res 112(1):113–121. doi:10.1007/s00436-012-3112-6
Baek M, Choy J-H, Choi S-J (2012) Montmorillonite intercalated with glutathione for antioxidant delivery: synthesis, characterization, and bioavailability evaluation. Int J Pharm 425(1–2):29–34. doi:10.1016/j.ijpharm.2012.01.015
Johnson J, Reid WM (1970) Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol 28(1):30–36. doi:10.1016/0014-4894(70)90063-9
Joshi GV, Kevadiya BD, Patel HA, Bajaj HC, Jasra RV (2009a) Montmorillonite as a drug delivery system: intercalation and in vitro release of timolol maleate. Int J Pharm 374(1–2):53–57. doi:10.1016/j.ijpharm.2009.03.004
Joshi GV, Patel HA, Kevadiya BD, Bajaj HC (2009b) Montmorillonite intercalated with vitamin B-1 as drug carrier. Appl Clay Sci 45(4):248–253. doi:10.1016/j.clay.2009.06.001
Joshi GV, Kevadiya BD, Bajaj HC (2010) Controlled release formulation of ranitidine-containing montmorillonite and Eudragit E-100. Drug Dev Ind Pharm 36(9):1046–1053. doi:10.3109/03639041003642073
Lee H-A, Hong S, Chung Y-H, Song K-D, Kim O (2012) Anticoccidial effects of Galla rhois extract on Eimeria tenella-infected chicken. Lab Anim Res 28(3):193–197. doi:10.5625/lar.2012.28.3.193
Lin FH, Lee YH, Jian CH, Wong JM, Shieh MJ, Wang CY (2002) A study of purified montmorillonite intercalated with 5-fluorouracil as drug carrier. Biomaterials 23(9):1981–1987
Liu Y, Zheng J, Li J, Gong P, Zhang X (2013) Protective immunity induced by a DNA vaccine encoding Eimeria tenella rhomboid against homologous challenge. Parasitol Res 112(1):251–257. doi:10.1007/s00436-012-3132-2
Ma DX et al (2011) Vaccination of chickens with DNA vaccine encoding Eimeria acervulina 3-1E and chicken IL-15 offers protection against homologous challenge. Exp Parasitol 127(1):208–214. doi:10.1016/j.exppara.2010.07.015
Meng N, Zhou NL, Zhang SQ, Shen J (2009) Controlled release and antibacterial activity chlorhexidine acetate (CA) intercalated in montmorillonite. Int J Pharm 382(1–2):45–49. doi:10.1016/j.ijpharm.2009.08.004
Nanda R, Sasmal A, Nayak PL (2011) Preparation and characterization of chitosan-polylactide composites blended with Cloisite 30B for control release of the anticancer drug paclitaxel. Carbohydr Polym 83(2):988–994. doi:10.1016/j.carbpol.2010.09.009
Park JK, Choy YB, Oh JM, Kim JY, Hwang SJ, Choy JH (2008) Controlled release of donepezil intercalated in smectite clays. Int J Pharm 359(1–2):198–204. doi:10.1016/j.ijpharm.2008.04.012
Pogonka T, Klotz C, Kovacs F, Lucius R (2003) A single dose of recombinant Salmonella typhimurium induces specific humoral immune responses against heterologous Eimeria tenella antigens in chicken. Int J Parasitol 33(1):81–88. doi:10.1016/s0020-7519(02)00251-5
Qi NS et al (2013) Partial protective of chickens against Eimeria tenella challenge with recombinant EtMIC-1 antigen. Parasitol Res 112(6):2281–2287. doi:10.1007/s00436-013-3389-0
Rambozzi L, Renna M, Cornale P, Perona G, Malfatto V, Mimosi A (2012) Effect of the granulometric characteristics of monensin sodium on controlling experimental coccidiosis in broiler chickens. Rev Bras Parasitol Vet 21(1):60–64
Sun B, Ranganathan B, Feng SS (2008) Multifunctional poly(D, L-lactide-co-glycolide)/montmorillonite (PLGA/MMT) nanoparticles decorated by Trastuzumab for targeted chemotherapy of breast cancer. Biomaterials 29(4):475–486. doi:10.1016/j.biomaterials.2007.09.038
Turhan Y, Alp ZG, Alkan M, Dogan M (2013) Preparation and characterization of poly(vinylalcohol)/modified bentonite nanocomposites. Microporous Mesoporous Mater 174:144–153. doi:10.1016/j.micromeso.2013.03.002
Wang CJ, Li ZH, Jiang WT, Jean JS, Liu CC (2010) Cation exchange interaction between antibiotic ciprofloxacin and montmorillonite. J Hazard Mater 183(1–3):309–314. doi:10.1016/j.jhazmat.2010.07.025
Wang JP, Chi F, Kim IH (2012) Effects of montmorillonite clay on growth performance, nutrient digestibility, vulva size, faecal microflora, and oxidative stress in weaning gilts challenged with zearalenone. Anim Feed Sci Technol 178(3–4):158–166. doi:10.1016/j.anifeedsci.2012.09.004
Webster GK, Pastore RJ, Hawkins KA, Hirsch AL (1998) Chemical reduction of zoalene to ANOT for use in zoalene residue studies. J Agric Food Chem 46(9):3623–3629. doi:10.1021/jf9802068
Wu YL, Xu Y, Yang T, Huang-Fu WG (2011) Simultaneous determination of zoalene and its metabolite in chicken muscle and liver by SPE and UPLC-MS-MS. Chromatographia 74(11–12):833–838. doi:10.1007/s10337-011-2142-z
Zhang DF et al (2012a) Anticoccidial effect of halofuginone hydrobromide against Eimeria tenella with associated histology. Parasitol Res 111(2):695–701. doi:10.1007/s00436-012-2889-7
Zhang DF et al (2012b) Adjuvant effect of ginsenoside-based nanoparticles (ginsomes) on the recombinant vaccine against Eimeria tenella in chickens. Parasitol Res 110(6):2445–2453. doi:10.1007/s00436-011-2784-7
Acknowledgements
This work was supported by grants from Major Science and Technology Projects of Zhejiang Province (grant no. 2008C12078), Zhejiang Gongshang University (grant no. 3080XJ2513204), National Innovation Training project (grant no. 3080JQ4313012), Foundation of Zhejiang Gongshang University (grant no. 3080XJ2513184).
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Qu, D., Ma, W., Ye, Y. et al. Effect of dinitolmide intercalated into Montmorillonite on E. tenella infection in chickens. Parasitol Res 113, 1233–1238 (2014). https://doi.org/10.1007/s00436-014-3762-7
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DOI: https://doi.org/10.1007/s00436-014-3762-7