Parasitology Research

, Volume 113, Issue 3, pp 1127–1134 | Cite as

Evaluation of the repellent effects of Nepeta parnassica extract, essential oil, and its major nepetalactone metabolite against mosquitoes

  • G. Gkinis
  • A. Michaelakis
  • G. Koliopoulos
  • E. Ioannou
  • O. TzakouEmail author
  • V. Roussis
Original Paper


The dichloromethane-methanol extract, the essential oil obtained by hydrodistillation from Nepeta parnassica, and the isolated 4aα,7α,7aβ-nepetalactone were evaluated for their repellent effect against the mosquitoes Aedes (Stegomyia) cretinus Edwards and Culex pipiens pipiens biotype molestus Forskål. The chemical analysis of N. parnassica essential oil, dominated by oxygenated monoterpenes (87 %), revealed 4aα,7α,7aβ-nepetalactone (36.8 %), 1,8-cineole (25.5 %), and 4aα,7β,7aβ-nepetalactone (11.1 %) as the major constituents. The results of the insect bioassays showed that the essential oil and the dichloromethane-methanol extract of N. parnassica were very active against Aedes cretinus for up to 3 h and against Culex pipiens for up to 2 h post application. The isolated 4aα,7α,7aβ-nepetalactone showed very high mosquito repellency for periods of at least 2 h against both species.


Mosquito Species Deet Eucalyptol Culex Pipiens Mosquito Repellent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to thank Dr. Theophanis Constandinidis (Department of Ecology and Systematics, Faculty of Biology, National and Kapodistrian University of Athens) for the plant identification.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • G. Gkinis
    • 1
  • A. Michaelakis
    • 2
  • G. Koliopoulos
    • 3
  • E. Ioannou
    • 1
  • O. Tzakou
    • 1
    Email author
  • V. Roussis
    • 1
  1. 1.Department of Pharmacognosy and Chemistry of Natural Products, School of PharmacyUniversity of AthensAthensGreece
  2. 2.Laboratory of Agricultural Entomology, Department of Entomology and Agricultural ZoologyBenaki Phytopathological InstituteAthensGreece
  3. 3.Laboratory of Biological Control of PesticidesBenaki Phytopathological InstituteAthensGreece

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