Skip to main content
SpringerLink
Log in

Sequencing the complete mitochondrial genome of Eimeria mitis strain USDA 50 (Apicomplexa: Eimeriidae) suggests conserved start positions for mtCOI- and mtCOIII-coding regions

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Four complete mitochondrial (mt) sequences from a single-oocyst-derived line of Eimeria mitis USDA 50 were obtained (three from cloned whole-genome PCR products, one from directly sequenced whole-genome PCR product). The mt genome is 6,408 bp long with three genes (CytB, cytochrome c oxidase subunit I (COI) and cytochrome c oxidase subunit III (COIII)) and many rDNA fragments (large subunit rDNA 13, small subunit rDNA 10); organisation was identical to other Eimeria sp. mt genomes. Conserved start codon positions for both COI and COIII are suggested for all Eimeria mt genomes; these start codon positions exist and may also be conserved, in related apicomplexan parasites. Within the three separate cloned PCR products of near-complete mt genomes, there were 26 nucleotide differences (collectively) compared to the directly sequenced mt genome. These changes appear to be base misincorporations during PCR. Direct sequencing of long PCR amplification products may be more likely to generate accurate mt genomic sequences than cloning and subsequent sequencing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H (1997) Phylogenetic relationship among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 83:262–271

    Article  PubMed  CAS  Google Scholar 

  • Bera AK, Bhattacharya D, Pan D, Dhara A, Kumar S, Das SK (2010) Evaluation of economic losses due to coccidiosis in poultry industry in India. Agric Econ Res Rev 23:91–96

    Google Scholar 

  • Campbell TN, Choy FYM (2001) Large-scale colony screening and insert orientation determination using PCR. BioTech 30:32–34

    CAS  Google Scholar 

  • Costa CA, Gomes RF, Melo MN, Ribeiro MF (2001) Eimeria parasites of domestic fowl: genetic relationships of different isolates estimated from random amplified polymorphic DNA. Parasitol Res 87:459–466

    Article  PubMed  CAS  Google Scholar 

  • Dalloul RA, Lillehoj HS (2006) Poultry coccidiosis: recent advancements in control measures and vaccine development. Expert Rev Vaccines 5:143–163

    Article  PubMed  CAS  Google Scholar 

  • Feagin JE (1994) The extrachromosomal DNAs of apicomplexan parasites. Annu Rev Microbiol 48:81–104

    Article  PubMed  CAS  Google Scholar 

  • Feagin JE (2000) Mitochondrial genome diversity in parasites. Int J Parasitol 30:371–390

    Article  PubMed  CAS  Google Scholar 

  • Feagin JE, Werner E, Gardner MJ, Williamson DH, Wilson RJM (1992) Homologies between the contiguous and fragmented rRNAs of the two Plasmodium falciparum extrachromosomal DNAs are limited to core sequences. Nucleic Acids Res 20:879–887

    Article  PubMed  CAS  Google Scholar 

  • Feagin JE, Harrell MI, Lee JC, Coe KJ, Sands BH, Cannone JJ, Tami G, Schnare MN, Gutell RR (2012) The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum. PLoS ONE 7(6):e38320. doi:10.1371/journalpone0038320

    Article  PubMed  Google Scholar 

  • Fernandez S, Pagotto AH, Furtado MM, Katsuyama AM, Madeira AMBN, Gruber AA (2003) Multiplex PCR assay for the simultaneous detection and discrimination of the seven Eimeria species that infect domestic fowl. Parasitology 127:317–325

    Article  PubMed  CAS  Google Scholar 

  • Gray MW, Burger G, Lang BF (1999) Mitochondrial evolution. Science 283:1476–1481

    Article  PubMed  CAS  Google Scholar 

  • Gray MW, Burger G, Lang BF (2001) The origin and early evolution of mitochondria. Genome Biol 2:1018.1–1018.5

    Article  Google Scholar 

  • Gray MW, Lang BF, Burger G (2004) Mitochondria of protists. Annu Rev Genet 38:477–524

    Article  PubMed  CAS  Google Scholar 

  • Hikosaka K, WatanabeY TN, Kita K, Kishine H, Arisue N, Nirianne MQ, Palacpac SK, Sawai H, Horii T, Igarashi I, Tanabe K (2010) Divergence of the mitochondrial genome structure in the apicomplexan parasites, Babesia and Theileria. Mol Biol Evol 27:1107–1116

    Article  PubMed  CAS  Google Scholar 

  • Hikosaka K, Nakai Y, Watanabe Y, Tachibana S, Arisue N, Palacpac NM, Toyama T, Honma H, Horii T, Kita K, Tanabe K (2011) Concatenated mitochondrial DNA of the coccidian parasite Eimeria tenella. Mitochondrion 11:273–278

    Article  PubMed  CAS  Google Scholar 

  • Kairo A, Fairlamb AH, Gobright E, Nene V (1994) A 7.1 kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins. EMBO (Eur Mol Biol Organ) J 13:898–905

    CAS  Google Scholar 

  • Lin RQ, Qiu LL, Liu GH, Wu XY, Weng YB, Xie WQ, Hou J, Pan H, Yuan ZG, Zou FC, Hu M, Zhu XQ (2011) Characterization of the complete mitochondrial genomes of five Eimeria species from domestic chickens. Gene 408:23–33

    Google Scholar 

  • Liu GH, Hou J, Weng YB, Song HQ, Li S, Yuan ZG, Lin RQ, Zhu X (2012) The complete mitochondrial genome sequence of Eimeria mitis (Apicomplexa: Coccidia). Mitochondrial DNA 23:341–343

    Article  PubMed  CAS  Google Scholar 

  • McDougald LR, Reid WM (1997) Protozoa: Coccidiosis. In: Calnek BW, Barnes HJ, Beard CW, McDougald LR, Saif YM (eds) Diseases of poultry. Iowa State University Press, Ames, pp 865–883

    Google Scholar 

  • Ogedengbe JD, Hanner RH, Barta JR (2011) DNA barcoding identifies Eimeria species and contributes to the phylogenetics of coccidian parasites (Eimeriorina, Apicomplexa, Alveolata). Int J Parasitol 41:843–850

    Article  PubMed  CAS  Google Scholar 

  • Olivieri C, Ermini L, Rizzi E, Corti G, Bonnai R, Luciani S, Marota I, De Bellis G, Rollo F (2010) Characterization of nucleotide misincorporation patterns in the Iceman's mitochondrial DNA. PLoS ONE 5:e8629. doi:10.1371/journal.pone.0008629.pg1-9

    Article  PubMed  Google Scholar 

  • Preiser PR, Wilson RJ, Moore PW, McCready S, Hajibagheri MA, Blight KJ, Strath M, Wiliamson DH (1996) Recombination associated with replication of malarial mitochondrial DNA. EMBO (Eur Mol Biol Organ) J 15:684–686

    CAS  Google Scholar 

  • Reid WM, Long PL (1979) A diagnostic chart for nine species of fowl coccidia. Univ GA Coll Agric Res Rep 335:1–24

    Google Scholar 

  • Remmler O, McGregor JK (1964) A method to facilitate isolation of single coccidial oocysts. J Parasitol 50:294

    Article  PubMed  CAS  Google Scholar 

  • Ryley JF, Meade R, Hazelhurst J, Robinson TE (1996) Methods in coccidiosis research: separation of oocysts from faeces. Parasitology 73:311–326

    Article  Google Scholar 

  • Shirley MW, Jeffers TK, Long PL (1983) Studies to determine the taxonomic status of Eimeria mitis, Tyzzer 1929 and E. mivati, Edgar and Seibold 1964. Parasitology 87:185–198

    Article  PubMed  Google Scholar 

  • Suplick K, Morrisey J, Vaidya AB (1990) Complex transcription from the extrachromosomal DNA encoding mitochondrial functions of Plasmodium yoelii. Mol Cell Biol 10:6381–6388

    PubMed  CAS  Google Scholar 

  • Turgut-Balik D, Celik VC, Moreton K, Brady RL (2005) Overcoming cloning problems by staining agarose gels with crystal violet instead of ethidium bromide in lactate dehydrogenase gene from Plasmodium vivax and Plasmodium falciparum. Acta Biol Hung 56:389–397

    Article  PubMed  CAS  Google Scholar 

  • Wilson RJ, Williamson DH (1997) Extrachromosomal DNA in the Apicomplexa. Microbiol Mol Biol Rev 61:1–16

    PubMed  CAS  Google Scholar 

  • Yang Y, Jung DW, Bai DG, Yoo GS, Choi JK (2001) Counterion-dye staining method for DNA in agarose gels using crystal violet and methyl orange. Electrophoresis 22:855–859

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Ontario Ministry of Agriculture and Food (OMAF) to JRB and scholarship support to MEO from the Ontario Veterinary College. Julia Whale and Julie Cobean are thanked for their technical assistance.

Author information

Authors and Affiliations

  1. Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada

    M. E. Ogedengbe, M. A. Hafeez & J. R. Barta

Authors
  1. M. E. Ogedengbe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Hafeez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. R. Barta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. R. Barta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ogedengbe, M.E., Hafeez, M.A. & Barta, J.R. Sequencing the complete mitochondrial genome of Eimeria mitis strain USDA 50 (Apicomplexa: Eimeriidae) suggests conserved start positions for mtCOI- and mtCOIII-coding regions. Parasitol Res 112, 4129–4136 (2013). https://doi.org/10.1007/s00436-013-3604-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-013-3604-z

Keywords

Search

Navigation