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Screening of plant extracts for anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

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Abstract

With the aim of finding natural anthelmintic agents against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus), 26 plants were screened for antiparasitic properties using in vivo anthelmintic efficacy assay. The results showed that Caesalpinia sappan, Lysima chiachristinae, Cuscuta chinensis, Artemisia argyi, and Eupatorium fortunei were found to have 100 % anthelmintic efficacy at 125, 150, 225, 300, and 500 mg L−1 after 48 h of exposure. Crude extract of the five plants were further partitioned with petroleum ether, chloroform, ethyl acetate, methanol, and water to obtain anthelmintically active fractions with various polarity. Among these fractions tested, the ethyl acetate extract of L. chiachristinae was found to be the most effective with a 50 % effective concentration (EC50) value of 5.1 mg/L after 48 h of exposure. This was followed by ethyl acetate extract of C. chinensis (48 h-EC50 = 8.5 mg L−1), chloroform extracts of C. sappan (48 h-EC50 = 15.6 mg L−1), methanol extract of C. chinensis (48 h-EC50 = 15.9 mg L−1), and chloroform and petroleum ether extract of L. chiachristinae (EC50 values of 17.2 and 21.1 mg/L, respectively), suggesting that these plants, as well as the active fractions, provide potential sources of botanic drugs for the control of D. intermedius in aquaculture.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program) (no. 2011AA10A216).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China

    Ai-Guo Huang, Fei Ling, Lin Lu & Gao-Xue Wang

  2. College of Science, Northwest A&F University, Yangling, 712100, China

    Yang-Lei Yi

  3. Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Minister of Education, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China

    Qi-Zhong Zhang

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  1. Ai-Guo Huang
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  2. Yang-Lei Yi
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  3. Fei Ling
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  4. Lin Lu
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  5. Qi-Zhong Zhang
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  6. Gao-Xue Wang
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Correspondence to Qi-Zhong Zhang or Gao-Xue Wang.

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Ai-Guo Huang and Yang-Lei Yi contributed equally to this work.

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Huang, AG., Yi, YL., Ling, F. et al. Screening of plant extracts for anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitol Res 112, 4065–4072 (2013). https://doi.org/10.1007/s00436-013-3597-7

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  • DOI: https://doi.org/10.1007/s00436-013-3597-7

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