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Phylogenetic analysis of Demodex caprae based on mitochondrial 16S rDNA sequence

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Abstract

Demodex caprae infests the hair follicles and sebaceous glands of goats worldwide, which not only seriously impairs goat farming, but also causes a big economic loss. However, there are few reports on the DNA level of D. caprae. To reveal the taxonomic position of D. caprae within the genus Demodex, the present study conducted phylogenetic analysis of D. caprae based on mt16S rDNA sequence data. D. caprae adults and eggs were obtained from a skin nodule of the goat suffering demodicidosis. The mt16S rDNA sequences of individual mite were amplified using specific primers, and then cloned, sequenced, and aligned. The sequence divergence, genetic distance, and transition/transversion rate were computed, and the phylogenetic trees in Demodex were reconstructed. Results revealed the 339-bp partial sequences of six D. caprae isolates were obtained, and the sequence identity was 100 % among isolates. The pairwise divergences between D. caprae and Demodex canis or Demodex folliculorum or Demodex brevis were 22.2–24.0 %, 24.0–24.9 %, and 22.9–23.2 %, respectively. The corresponding average genetic distances were 2.840, 2.926, and 2.665, and the average transition/transversion rates were 0.70, 0.55, and 0.54, respectively. The divergences, genetic distances, and transition/transversion rates of D. caprae versus the other three species all reached interspecies level. The five phylogenetic trees all presented that D. caprae clustered with D. brevis first, and then with D. canis, D. folliculorum, and Demodex injai in sequence. In conclusion, D. caprae is an independent species, and it is closer to D. brevis than to D. canis, D. folliculorum, or D. injai.

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References

  • Brugger M, Braun U (2000) Demodicosis in a Toggenburger goat. Schweiz Arch Tierh 142:639–642

    PubMed  CAS  Google Scholar 

  • Bukva V (1990) Three species of the hair follicle mites (Acari: Demodicidea) parasitizing the sheep, ovis aries L. Folia Parasitol 37:81–91

    PubMed  CAS  Google Scholar 

  • Chanie M, Negash T, Sirak A (2010) Ectoparasites are the major causes of various types of skin lesions in small ruminants in Ethiopia. Trop Anim Health Prod 42:1103–1109

    Article  PubMed  Google Scholar 

  • Chen ZH, Liu XY, Wu XF, 79 (1958) Demodex caprae (hair follicle mite) disease found in slaughterhouse of Shanghai. Shanghai Anim Husb Vet Med 2:3–4

    Google Scholar 

  • Chen NQ (1999) An investigation of goat demodicidosis in north Guizhou. Chin J Vet Parasitol 7:37

    Google Scholar 

  • de Rojas M, Riazzo C, Callejón R, Guevara D, Cutillas C (2012a) Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans. Parasitol Res 110:227–233

    Article  PubMed  Google Scholar 

  • de Rojas M, Riazzo C, Callejón R, Guevara D, Cutillas C (2012b) Molecular study on three morphotypes of Demodex mites (Acarina: Demodicidae) from dogs. Parasitol Res 111:2165–2172

    Article  PubMed  Google Scholar 

  • Du LY, Gong CE, Luo YH, Shi JX, Xiang CB, Liu XJ, He MD (1999) Investigation on the infection of Demodex mites of goats in border region of Hunan and Guizhou. Chin J Vet Sci Technol 29:31

    Google Scholar 

  • Fleischer P, Lukesova D, Skrivanek M, Hofirek B, Stursa I (1996) First cases of caprine demodectic mange in the Czech Republic. Vet Med-Czech 41:289–293

    CAS  Google Scholar 

  • Gjerde B, Kalstad H (1999) Nodular demodicosis in Norwegian goats caused by Demodex caprae. Norsk Veterinaertidsskrift 111:549–552

    Google Scholar 

  • Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Nei M, Dusley J, Tamura K (2008) MEGA: a biologistcentric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306

    Article  PubMed  CAS  Google Scholar 

  • Liu YR, Liu FH, Xiao KY, Guo SL, Feng YX, Yuan FS (2012) Reaction system optimization and primer screening in ISSR-PCR. J Shandong Univ (Health Sci) 50:57–61

    CAS  Google Scholar 

  • Milosevic MA, Frank LA, Brahmbhatt RA, Kania SA (2013) PCR amplification and DNA sequencing of Demodex injai from otic secretions of a dog. Vet Dermatol 24:286–e66

    Article  PubMed  Google Scholar 

  • Mulugeta Y, Yacob HT, Ashenafi H (2010) Ectoparasites of small ruminants in three selected agro-ecological sites of Tigray Region, Ethiopia. Trop Anim Health Prod 42:1219–1224

    Article  PubMed  CAS  Google Scholar 

  • Sastre N, Ravera I, Villanueva S, Altet L, Bardagí M, Sánchez A, Francino O, Ferrer L (2012) Phylogenetic relationships in three species of canine Demodex mite based on partial sequences of mitochondrial 16S rDNA. Vet Dermatol 23:509–e101

    Article  PubMed  Google Scholar 

  • Silbermayr K, Joachim A, Litschauer B, Panakova L, Sastre N, Ferrer L, Horvath-Ungerboeck C (2013) The first case of Demodex gatoi in Austria, detected with fecal flotation. Parasitol Res 112:2805–2810

    Article  PubMed  Google Scholar 

  • Strabel D, Schweizer G, Gansohr B, Braun U (2003) Treatment with avermectins in two goats with demodicosis. Schweiz Arch Tierh 145:585–587

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Wei CF, Wu WH (1992) The endemic distribution and risk of infection of goat demodicidosis. Guizhou Anim Husb Vet Med 3:20–21

    Google Scholar 

  • Wu CM, Lai CL (1997) Scanning electron microscopic observation of Demodex caprae. J Parasitol Med Entomol 4:228–231

    Google Scholar 

  • Xiao KY, Guo SL, Liu YR, Su L, Feng YX, Yuan FS (2012) Construction and identification of cDNA Library of Demodex mites. J Shandong Univ (Health Sci) 50:15–19

    Google Scholar 

  • Xu HZ, Wei SF, Wu WH, Xiao FP (2000) Application of dot enzyme-linked immunosorbent assay for diagnosis of goat demodicidosis. Guizhou Anim Husb Vet Med 24:1–2

    Google Scholar 

  • Xu HZ, Xiao FP (2002) The early immunologic diagnosis test of goat demodicidosis. Chin J Vet Sci Technol 32:28–30

    Google Scholar 

  • Xu HZ, Xiao FP, Qin ZY, Ye XF, Ai YP (2009) Early diagnosis, prevention and cure of goat demodicidosis. Guizhou Agric Sci 37:125–126

    CAS  Google Scholar 

  • Yacob HT, Yalew TA, Dinka AA (2008a) Part I: Ectoparasite prevalences in sheep and in goats in and around Wolaita Soddo, Southern Ethiopia. Rev Med Vet-Toulouse 159:450–454

    Google Scholar 

  • Yacob HT, Nesanet B, Dinka A (2008b) Part II: Prevalences of major skin diseases in cattle, sheep and goats at Adama Veterinary Clinic, Oromia regional state, Ethiopia. Rev Med Vet-Toulouse 159:455–461

    Google Scholar 

  • Yang MF, Chen DR, Chen DS, Jiang XL, Zhang CG, Liu SM, Guan GJ, Dai ZJ, Zhang JM (1983) A preliminary survey of goat demodicidosis in Sichuan. J Vet Sci & Technol 6: 32-33+67

    Google Scholar 

  • Yang L, Zhang J, Wang KG, Cheng ZT (2004) Diagnosis and control of goat demodicosi. Guizhou Anim Husb Vet Med 28:23

    Google Scholar 

  • Ye MZ, Fan PF (1994) Study on histochemistry and pathogenicity of Demodex caprae. Chin J Vet Parasitol 2:16–21

    Google Scholar 

  • Ye MZ, Fan PF (1995) Observation of internal ultrastructure in female Demodex caprae. J Shanghai Agri Coll 13:20–25

    Google Scholar 

  • Zhao Y (2011) Initial study on the extraction method of genomic DNA of Demodex caprae. Heilongjiang Anim Sci Vet Med 2:109–110

    Google Scholar 

  • Zhao YE, Cheng H (2009) RAPD analysis and sequence alignment of genomic DNA of hair follicle mites Demodex folliculorum and D. brevis (Acari: Demodicidae). Acta Entomol Sin 52:1273–1279

    CAS  Google Scholar 

  • Zhao YE, Cheng H, Xun M, Wu LP (2009) Extraction and random primer PCR detection of genomic DNA of parasitic mites Demodex folliculorum and Demodex brevis (Acari: Demodicidae). Acta Entomol Sin 52:929–933

    CAS  Google Scholar 

  • Zhao YE, Wu LP (2012a) RAPD-SCAR marker and genetic relationship analysis of three Demodex species (Acari: Demodicidae). Parasitol Res 110:2395–2402

    Article  PubMed  Google Scholar 

  • Zhao YE, Wu LP (2012b) Phylogenetic relationships in Demodex mites (Acari: Demodicidae) based on mitochondrial 16S rDNA partial sequences. Parasitol Res 111:1113–1121

    Article  PubMed  Google Scholar 

  • Zhao YE, Wu LP, Hu L, Xu Y, Wang ZH, Liu WY (2012a) Sequencing for complete rDNA sequences (18S, ITS1, 5.8S, ITS2, and 28S rDNA) of Demodex and phylogenetic analysis of Acari based on 18S and 28S rDNA. Parasitol Res 111:2109–2114

    Article  PubMed  Google Scholar 

  • Zhao YE, Xu JR, Hu L, Wu LP, Wang ZH (2012b) Complete sequence analysis of 18S rDNA based on genomic DNA extraction from individual Demodex mites (Acari: Demodicidae). Exp Parasitol 131:45–51

    Article  PubMed  CAS  Google Scholar 

  • Zhao YE, Hu L, Ma JX (2013a) Molecular identification of four phenotypes of human Demodex mites (Acari: Demodicidae) based on mitochondrial 16S rDNA. Parasitol Res. doi: 10.1007/s00436-013-3558-1

  • Zhao YE, Ma JX, Hu L, Wu LP, de Rojas M (2013b) Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences. J Zhejiang Univ Sci B 14:829–836

    Google Scholar 

  • Zhou JY, Zhang Q, Tang YL, Yu FY, Zhao S (2010) On phylogenetic relationships of Teraponidae in coastal waters of China. Marine Fisheries 32:351–355

    Google Scholar 

  • Zhou QZ (2004) Diagnosis and treatment of goat demodicidosis outbroken in a sheep farm. Chin J Vet Parasitol 12:56–58

    Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (81271856).

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Authors and Affiliations

  1. Department of Immunology and Pathogen Biology, Xi’an Jiaotong University College of Medicine, No.76 Yanta West Road, Xi’an, China, 710061

    Ya-E Zhao, Li Hu & Jun-Xian Ma

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  1. Ya-E Zhao
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  2. Li Hu
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  3. Jun-Xian Ma
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Correspondence to Ya-E Zhao.

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Zhao, YE., Hu, L. & Ma, JX. Phylogenetic analysis of Demodex caprae based on mitochondrial 16S rDNA sequence. Parasitol Res 112, 3969–3977 (2013). https://doi.org/10.1007/s00436-013-3586-x

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  • DOI: https://doi.org/10.1007/s00436-013-3586-x

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