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An immunoblot for detection of Taenia saginata cysticercosis

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Abstract

Control measures to prevent human infections with the food-borne zoonotic helminth Taenia saginata are currently based on meat inspection, which shows rather low diagnostic sensitivity. To develop an immunoblot for detection of T. saginata-infected cattle, crude proteins of T. saginata cysts were extracted and separated with SDS-PAGE. The cyst antigens showed ten protein bands ranging from 260 to 14 kDa. T. saginata cyst proteins 260, 150, 130, 67, 60, 55, 50, and 23 kDa were immunoreactive with known positive sera of T. saginata-infected cattle but cross-reacted with sera from Echinocccus granulosus-infected ruminants. By contrast, 14- and 18-kDa cyst proteins reacted specifically with T. saginata-positive sera and thus might be potential candidates for the development of a T. saginata-specific immunoassay. Proteins of E. granulosus cysts and Taenia hydatigena cysts were also extracted and separated with SDS-PAGE. E. granulosus cysts revealed 11 protein bands ranging from 260 to 23 kDa. E. granulosus protein 60 kDa was immunoreactive with E. granulosus-positive sera only. The cyst of T. hydatigena showed 11 protein bands ranging from 290 to 14 kDa. The protein band 35 kDa showed cross-reaction with positive sera from both T. saginata- and E. granulosus-infected animals. A protein of 67 kDa was present in all three tested cestode species and was the major antigenic protein detected by sera of T. saginata- and E. granulosus-infected animals. Therefore, this protein represents a potential vaccine candidate against both cysticercosis and cystic echinococcosis in cattle.

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Acknowledgments

The authors gratefully acknowledge the financial support of the German Academic Exchange Service (DAAD) under grant A/12/08212.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, An-Najah National University, P.O. Box 7, Nablus, West Bank, Palestine

    Sameh Abuseir

  2. Lower Saxony State Office for Consumer Protection and Food Safety, Food and Veterinary Institute, Hannover, Germany

    Uschi Nagel-Kohl

  3. Institute for Parasitology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany

    Sonja Wolken & Christina Strube

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  1. Sameh Abuseir
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  2. Uschi Nagel-Kohl
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  3. Sonja Wolken
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  4. Christina Strube
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Correspondence to Sameh Abuseir.

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Abuseir, S., Nagel-Kohl, U., Wolken, S. et al. An immunoblot for detection of Taenia saginata cysticercosis. Parasitol Res 112, 2069–2073 (2013). https://doi.org/10.1007/s00436-013-3368-5

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  • DOI: https://doi.org/10.1007/s00436-013-3368-5

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