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Essential oil composition and larvicidal activity of Toddalia asiatica roots against the mosquito Aedes albopictus (Diptera: Culicidae)

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Abstract

The aim of this research was to determine larvicidal activity of the essential oil derived from roots of Toddalia asiatica (L.) Lam. and the isolated constituents against the larvae of the Culicidae mosquito Aedes albopictus. Essential oil of T. asiatica roots was obtained by hydrodistillation and analyzed by GC and GC-MS. A total of 58 components of the essential oil of T. asiatica roots were identified. The essential oil has almost same content of (35.41 %) of sesquiterpenoids and monoterpenoids (31.87 %). The principal compounds in T. asiatica essential oil were geraniol (9.84 %), d-limonene (7.52 %), isopimpinellin (6.62 %), α-gurjunene (6.25 %), and 4-vinylguaiacol (5.94 %). Based on bioactivity-directed fractionation, geraniol, d-limonene, and isopimpinellin were isolated from T. asiatica root essential oil. Geraniol, d-limonene, and isopimpinellin exhibited strong larvicidal activity against A. albopictus with LC50 values of 30.13, 19.84, and 32.05 μg/ml, respectively, while the essential oil of T. asiatica had an LC50 value of 69.09 μg/ml. The result indicated that the essential oil of T. asiatica roots and the three isolated constituents have potential for use in control of A. albopictus larvae and could be useful in search of newer, safer, and more effective natural compounds as larvicides.

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Acknowledgments

This work was funded by the Hi-Tech Research and Development of China 2011AA10A202 and 2006AA10A209. We thank Dr. QR Liu from the College of Life Sciences, Beijing Normal University, Beijing 100875, for the identification of the investigated medicinal herb.

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Authors and Affiliations

  1. Department of Entomology, China Agricultural University, Haidian District, Beijing, 100193, China

    Xin Chao Liu & Zhi Long Liu

  2. Department of Biology, Taiyuan Normal University, Taiyuan, Shanxi, 030031, China

    Hui Wen Dong

  3. Department of Plant Pathology, China Agricultural University, Haidian District, Beijing, 100193, China

    Ligang Zhou

  4. State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Haidian District, Beijing, 100875, China

    Shu Shan Du

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  1. Xin Chao Liu
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Correspondence to Zhi Long Liu.

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Liu, X.C., Dong, H.W., Zhou, L. et al. Essential oil composition and larvicidal activity of Toddalia asiatica roots against the mosquito Aedes albopictus (Diptera: Culicidae). Parasitol Res 112, 1197–1203 (2013). https://doi.org/10.1007/s00436-012-3251-9

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