Skip to main content
SpringerLink
Log in

The mRNA level of the galectin-10 of Angiostrongylus cantonensis induced by reactive oxygen stress

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Galectin plays an important role in host–parasite interactions. In this study, we identified a novel gene encoding galectin-10 (AcGal-10) from the cDNA library of Angiostrongylus cantonensis and characterized its biological role in the parasite. Sequence and phylogeny analysis showed that AcGal-10 is related to other galectin family members with the conserved loci (H84-D86-R88-V96-N98-W105-E108-R110). The mRNA level of AcGal-10 was expressed in reactive oxygen stress radicals. We have identified two proteins of A. cantonensis galectin-10 gene, one of which was reported (AcGAL10-W) and the others is AcGAL-10-M. In addition, recombinant AcGal-10 (rAcGal-10) was constructed into the pGEX-4T-1 plasmid, purified, and finally confirmed by SDS-PAGE and LC-MS. Hemagglutination assay showed that the minimum concentration of rAcGAL10-W and rAcGAL10-M required for the hemagglutination of BALB/c mice erythrocyte was 25 μg/mL, and the carbohydrate-binding ability showed no difference between rAcGAL10-W and rAcGAL10-M. The mRNA levels of AcGal-10 were indeed expressed higher after stimulation with H2O2 and recombinant A. cantonensis galectin-10. A mutation of AcGal-10 was also found, but there was no significant difference compared with the wild type. Furthermore, we also confirmed that recombinant AcGal-10 plays a role in the activation of the microglia. In conclusion, the report here showed that AcGal-10 may be an important molecule related to infection of A. cantonensis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  • Alto W (2001) Human infections with Angiostrongylus cantonensis. Pac Health Dialog 8:176–182

    PubMed  CAS  Google Scholar 

  • Alves CM, Silva D, Azzolini AE et al (2009) Galectin-3 plays a modulatory role in the life span and activation of murine neutrophils during early Toxoplasma gondii infection. Immunobiology 215:475–485

    Article  PubMed  Google Scholar 

  • Beaver PC, Rosen L (1964) Memorandum on the first report of Angiostrongylus in man, by Nomura and Lin. Am J Trop Med Hyg 13:589–590

    PubMed  CAS  Google Scholar 

  • Chen D, Zhang Y, Shen H, Wei Y, Huang D, Tan Q, Lan X, Li Q, Chen Z, Li Z, Ou L, Suen H, Ding X, Luo X, Li X, Zhan X (2011) Epidemiological survey of Angiostrongylus cantonensis in the west-central region of Guangdong Province, China. Parasitol Res 109:305–314

    Article  PubMed  Google Scholar 

  • Cheng M, Yang X et al (2012) Cloning and characterization of a novel cathepsin B-like cysteine proteinase from Angiostrongylus cantonensis. Parasitol Res 110:2413–2422

    Article  PubMed  Google Scholar 

  • Chung LY, Chen C, Wang LC et al (2010a) Oxidative stress in mice infected with Angiostrongylus cantonensis coincides with enhanced glutathione-dependent enzymes activity. Exp Parasitol 126:178–183

    Article  PubMed  CAS  Google Scholar 

  • Chung LY, Wang LC et al (2010b) Kinetic change of oxidative stress in cerebrospinal fluid of mice infected with Angiostrongylus cantonensis. Redox Rep 15:43–48

    Article  PubMed  CAS  Google Scholar 

  • Fang W, Wang Y et al (2010) ES proteins analysis of Angiostrongylus cantonensis: products of the potential parasitism genes? Parasitol Res 106:1027–1032

    Article  PubMed  Google Scholar 

  • Harrop SA, Prociv P, Brindley PJ (1995) Amplification and characterization of cysteine proteinase genes from nematodes. Trop Med Parasitol 46:119–122

    PubMed  CAS  Google Scholar 

  • He JZ, Zhu S, Yang SQ et al (1984) First discovery and evidence of Angiostrongylus cantonensis in the cerebrospinal fluid from a case of the population of Mainland China. J Guangzhou Med Coll 12:1–4

    CAS  Google Scholar 

  • Hwang KP, Chen ER (1991) Clinical studies on angiostrongyliasis cantonensis among children in Taiwan. Southeast Asian J Trop Med Public Health 22(Suppl):194–199

    PubMed  Google Scholar 

  • Kiffin R, Christian C, Knecht E, Cuervo AM (2004) Activation of chaperone-mediated autophagy during oxidative stress. Mol Biol Cell 15:4829–4840

    Article  PubMed  CAS  Google Scholar 

  • Kurz TTA, Gustafsson B, Brunk UT (2008) Lysosomes and oxidative stress in aging and apoptosis. Biochim Biophys Acta 1780:1291–1303

    Article  PubMed  CAS  Google Scholar 

  • Lai K et al (2009) Differences of proteolytic enzymes and pathological changes in permissive and nonpermissive animal hosts for Angiostrongylus cantonensis infection. Vet Parasitol 165:265–272

    Article  PubMed  Google Scholar 

  • Lee JD, Chang JH et al (1996) Role of T cell subpopulations in mice infected with Angiostrongylus cantonensis. J Helminthol 70:211–214

    Article  PubMed  CAS  Google Scholar 

  • Li ZY, Lv ZY, Wei J, Liao Q, Zheng HQ, Wu ZD (2012) Cloning and characterization of a novel gene encoding 16 kDa protein (Ac16) from Angiostrongylus cantonensis. Parasitol Res 110:2145–2153

    Article  PubMed  Google Scholar 

  • Liang HK, Shen H, Li L (2005) Study on pathogenicity of Angiostrongylus cantonensis in laboratory animals. Acad J Guangzhou Med Coll 12:38–48

    Google Scholar 

  • Morassutti AL, Levert K, Pinto PM, da Silva AJ, Wilkins P, Graeff-Teixeira C (2012) Characterization of Angiostrongylus cantonensis excretory–secretory proteins as potential diagnostic targets. Exp Parasitol 130:26–31

    Article  PubMed  CAS  Google Scholar 

  • Ouyang L, Wei J, Wu Z, Zeng X, Li Y, Jia Y, Ma Y, Zhan M, Lei W (2012) Differences of larval development and pathological changes in permissive and nonpermissive rodent hosts for Angiostrongylus cantonensis infection. Parasitol Res 111:1547–1557

    Article  PubMed  Google Scholar 

  • Sanchez-Cuellar S, de la Fuente H, Cruz-Adalia A, Lamana A, Cibrian D, Giron RM, Vara A, Sanchez-Madrid F, Ancochea J (2012) Reduced expression of galectin-1 and galectin-9 by leucocytes in asthma patients. Clin Exp Immunol 170:365–374

    Article  PubMed  CAS  Google Scholar 

  • Sato S, St-Pierre C, Bhaumik P, Nieminen J (2009) Galectins in innate immunity: dual functions of host soluble beta-galactoside-binding lectins as damage-associated molecular patterns (PAMPs) and as receptors for pathogen-associated molecular patterns (PAMPs). Immunol Rev 230:172–187

    Article  PubMed  CAS  Google Scholar 

  • Schmittgen TD, Livak KJ (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 25:402–408

    Article  PubMed  Google Scholar 

  • Sun X, Liu Y, Lv ZY, Yang LL, Hu SM, Zheng HQ, Hu W, Cao JP, Fung MQ, Wu ZD (2010) rSj16, a recombinant protein of Schistosoma japonicum-derived molecule, reduces severity of the complete Freund’s adjuvant-induced adjuvant arthritis in rats’ model. Parasite Immunol 32:739–748

    Article  PubMed  CAS  Google Scholar 

  • Wei J, Wu F, Sun X, Zeng X, Liang JY, Zheng HQ, Yu XB, Zhang KX, Wu ZD (2012) Differences in microglia activation between rats-derived cell and mice-derived cell after stimulating by soluble antigen of IV larva from Angiostrongylus cantonensis in vitro. Parasitol Res. Accessed 17 Oct 2012

  • Weller PF, Liu L (1993) Eosinophilic meningitis. Semin Neurol 13:161–168

    Article  PubMed  CAS  Google Scholar 

  • Wilson LR, Good RT, Panaccio M, Wijffels GL, Sandeman RM, Spithill TW (1998) Fasciola hepatica: characterization and cloning of the major cathepsin B protease secreted by newly excysted juvenile liver fluke. Exp Parasitol 88:85–94

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Basic Research Program of China (2010CB530004) and National Nature Science Foundation of China (81271855, 81261160324).

Author information

Authors and Affiliations

  1. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China

    Ling-hui Liu, Zhi-yue Lv, Jie Wei, Xin Zeng, Jin-yi Liang, Huan-qin Zheng, Xin-bing Yu, Xi Sun & Zhong-dao Wu

  2. Key Laboratory of Tropical Disease Control, Ministry of Education, Guangzhou, 510080, Guangdong, China

    Ling-hui Liu, Zhi-yue Lv, Jie Wei, Xin Zeng, Jin-yi Liang, Huan-qin Zheng, Xin-bing Yu, Xi Sun & Zhong-dao Wu

  3. Department of Pathogenic Biology, Xiangnan University, Chenzhou, 423000, China

    Han-Jiang He

Authors
  1. Ling-hui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Han-Jiang He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhi-yue Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jie Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xin Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jin-yi Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Huan-qin Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xin-bing Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Xi Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Zhong-dao Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xi Sun or Zhong-dao Wu.

Additional information

Ling-Hui Liu and Han-Jiang He contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, Lh., He, HJ., Lv, Zy. et al. The mRNA level of the galectin-10 of Angiostrongylus cantonensis induced by reactive oxygen stress. Parasitol Res 112, 933–943 (2013). https://doi.org/10.1007/s00436-012-3210-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-012-3210-5

Keywords

Search

Navigation